Anti hoxa9

Manufactured by Merck Group

Anti-Hoxa9 is a laboratory product designed to detect the Hoxa9 protein. Hoxa9 is a transcription factor that plays a role in hematopoiesis and is associated with certain types of leukemia. Anti-Hoxa9 can be used in various research applications to study the expression and function of Hoxa9 in biological systems.

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Lab products found in correlation

Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg hrp, by Merck Group (1 mentions) Tris glycine gel, by Thermo Fisher Scientific (1 mentions) Anti myb, by Merck Group (1 mentions) Supersignal west chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Anti β actin, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions)

Spelling variants of this product

Rabbit anti-Hoxa9 (07-178, (2 citations)

2 protocols using anti hoxa9

Western Blot Analysis of Transcription Factors

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For Western blotting analysis, cells were washed twice with cold PBS and then whole cell lysates were prepared by direct lysis of cell pellets in heated 2X SDS sample buffer. Samples were resolved on 4–12% Tris-Glycine gels (Life Technologies, Carlsbad, CA) followed by transferring onto nitrocellulose membranes (Bio-Rad, Hercules, CA). Primary antibodies used include anti-Setbp1 (16841-1AP, Proteintech, Chicago, IL), anti-Myb (05–175, Millipore, Temecula, CA), anti-Hoxa9 (07–178, Millipore, Temecula, CA), and Anti-β-Actin (ab8224, Abcam, Cambridge, MA). Secondary antibodies used include goat anti-rabbit IgG-HRP (SC-2004, Santa Cruz Biotechnology, Dallas, TX) and anti-mouse IgG-HRP (A-9044, Sigma Aldrich). Protein bands were visualized by incubation with SuperSignal West chemiluminescent substrate (Pierce, Thermo Fisher Scientific, Rockford, IL) and quantified using Image Lab software (Bio-Rad, Hercules, CA).

Nguyen N., Vishwakarma B.A., Oakley K., Han Y., Przychodzen B., Maciejewski J.P, & Du Y. (2016). Myb expression is critical for myeloid leukemia development induced by Setbp1 activation. Oncotarget, 7(52), 86300-86312.

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Co-immunoprecipitation of Hox Complexes

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Co-IP was performed in IPH buffer using Agarose A beads (Santa Cruz Biotechnology, SC-2001). All equilibration and washes were performed with IPH buffer (50 mM Tris-HCl, 150 mM NaCl, 5mM EDTA, 0.5% NP-40) with freshly added protease inhibitor cocktail (Sigma Aldrich, St. Louis, #P8340).
Proteins were separated on 10% polyacrylamide gels and transferred to nitrocellulose membranes using Trans BlotTurbo transfer system (BioRad, Hercules, CA). Membranes were probed with anti-PREP1 (N15) (Santa Cruz Biotechnology, Santa Cruz, #SC-6245), anti-Pbx1/2/3 (C-20) (Santa Cruz Biotechnology, Santa Cruz# SC-888), anti-Meis1 (Milipore, Billerica, #05-779), anti-Hoxa9 (Millipore, Billerica, #07-178), or anti-beta-Actin (Sigma, St. Louis, #A5441). Secondary antibody probing was performed with TrueBlot anti-rabbit (eBioscience, San Diego, #18-8816-31), anti-mouse-HRP (GE Life sciences, Piscataway, #NA931VS), or anti-Rabbit-HRP (GE Life sciences, Piscataway, #NA934VS).

Mian Y.A, & Zeleznik-Le N.J. (2016). The miR-17~92 cluster contributes to MLL leukemia through the repression of MEIS1 competitor PKNOX1. Leukemia research, 46, 51-60.

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