Anti scap 9d5

Monoclonal anti-SREBP-1 (2A4), anti-SCAP (9D5), and anti-HSP90 (F-8) antibodies were purchased from Santa Cruz (Dallas, TX, USA). Monoclonal anti-FLAG (M2) and anti-β-actin (AC-15) antibodies were purchased from Sigma (St Louis, MO, USA). Monoclonal anti-HA (16B12) antibody was purchased from BioLegend (San Diego, CA, USA). The polyclonal anti-SREBP-2 (RS004) antibody has been previously described^{46 (link)}. Peroxidase-conjugated affinity-purified donkey anti-mouse IgGs and peroxidase-conjugated affinity-purified donkey anti-rabbit IgGs were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Samples of the ventral prostate obtained from five animals in each group were frozen, weighed, and homogenized in 50 mL/mg of lysis buffer. The technique used has been previously briefly described by Fávaro and Cagnon [27 (link)]. In brief, tissue slices were incubated with primary antibodies diluted in 1% BSA at 4 °C overnight. The following primary antibodies were used: anti-CD36 (L-17, Santa Cruz Biotechnology sc-5523), anti-SCAP (9D5, Santa Cruz Biotechnology sc-13,553), anti-LIMPII (D-4, Santa Cruz Biotechnology sc-55,571), anti-SREBP-1 (H-160, Santa Cruz Biotechnology sc-8984), and anti-caspase-3 (Abcam ab4051) at a dilutions of 1:500, 1:250, 1:250, 1:500, respectively; anti-β-actin (Abcam ab8227) was used as a control. An anti-goat secondary antibody (Santa Cruz Biotechnology sc-2354) was used against all primary antibodies at a 1:1000 dilution. The results are expressed as the mean of the ratio between the intensity of each band against the intensity of the β-actin band.