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Mouse g csf quantikine elisa kit

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The Mouse G-CSF Quantikine ELISA kit is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed to measure mouse Granulocyte Colony-Stimulating Factor (G-CSF) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Prism 6, by GraphPad (1 mentions) Mouse il 18 il 1f4 elisa kit, by R&D Systems (1 mentions) Mouse il 1β, by Thermo Fisher Scientific (1 mentions) Mouse il 6 quantikine elisa kit, by R&D Systems (1 mentions) Mouse il 17a quantikine elisa kit, by R&D Systems (1 mentions)

8 protocols using mouse g csf quantikine elisa kit

1

Quantification of G-CSF in Macrophage ELV Stimulation

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Triplicate wells of adhered J774A.1 cells, prepared as described above, were treated with LPS (final concentration 200 ng/mL or 0.003 ng/mL), approximately 4 × 108 purified L3 stage ELVs as described above, or RPMI 1640 as negative control. Cell culture supernatants were collected 24 hrs after treatment, cleared via centrifugation as described previously and assayed for G-CSF using a Mouse G-CSF Quantikine ELISA kit (R&D Systems, Minneapolis, MN). Standard curves were generated using Prism 6 software (GraphPad Software, San Diego, CA) and sample G-CSF concentrations determined by regression analysis.
Zamanian M., Fraser L.M., Agbedanu P.N., Harischandra H., Moorhead A.R., Day T.A., Bartholomay L.C, & Kimber M.J. (2015). Release of Small RNA-containing Exosome-like Vesicles from the Human Filarial Parasite Brugia malayi. PLoS Neglected Tropical Diseases, 9(9), e0004069.
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2

Quantifying Cytokine Levels in Serum

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Cytokines in the serum were analyzed using a Mouse G-CSF Quantikine ELISA kit (catalog #MCS00) and mouse IL-18/IL-1F4 ELISA kit (catalog #7625) according to the manufacturer’s instructions (R&D Systems, MN, USA).
Sasaki Y., Otsuka K., Arimochi H., Tsukumo S.I, & Yasutomo K. (2020). Distinct Roles of IL-1β and IL-18 in NLRC4-Induced Autoinflammation. Frontiers in Immunology, 11, 591713.
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3

Cytokine Quantification in Mice

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The concentration of IL-1β and IL-6 was measured with the mouse IL-1β or IL-6 ELISA Ready-Set-Go kit, respectively (eBioscience). The concentration of IL-17 was measured with a biotin-conjugated anti–mouse IL-17A antibody and a purified anti–mouse IL-17A antibody (BioLegend). The concentration of G-CSF was measured with the Mouse G-CSF Quantikine ELISA kit (R&D Systems).
Kitamura A., Sasaki Y., Abe T., Kano H, & Yasutomo K. (2014). An inherited mutation in NLRC4 causes autoinflammation in human and mice. The Journal of Experimental Medicine, 211(12), 2385-2396.
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4

Quantifying Serum G-CSF in Hematopoiesis

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Serum collected from the hematopoietic study was used to detect circulating levels of G-CSF using the mouse G-CSF Quantikine ELISA kit (R&D Systems, Minneapolis, MN) and following manufacturer’s instructions. The cytokine detection limit was 5 pg/ml and the G-CSF positive control was between 97.9–163.2 pg/ml. Data is represented as the mean ± SEM of n = 2–3 mice/group/time point for a total of 50 animals.
Jurgensen K.J., Skinner W.K., Oronsky B., Abrouk N.D., Graff A.E., Landes R.D., Culp W.E., Summers TA J.r, & Cary L.H. (2021). RRx-001 Radioprotection: Enhancement of Survival and Hematopoietic Recovery in Gamma-Irradiated Mice. Frontiers in Pharmacology, 12, 676396.
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5

Plasma G-CSF Level Quantification

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Blood was collected by retro-orbital bleeding and plasma was isolated using BD Microtainer Plastic Capillary Blood Collectors. Plasma G-CSF level was detected with Mouse G-CSF Quantikine ELISA Kit (R&D) following the manufacture’s instruction.
Jiao J., Dragomir A.C., Kocabayoglu P., Rahman A.H., Chow A., Hashimoto D., Leboeuf M., Kraus T., Moran T., Carrasco-Avino G., Friedman S.L., Merad M, & Aloman C. (2014). Central Role of Conventional Dendritic Cells in Regulation of Bone Marrow Release and Survival of Neutrophils. Journal of immunology (Baltimore, Md. : 1950), 192(7), 3374-3382.
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6

Quantification of Cytokines in Mouse Sera

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Blood sera from DKO*, DKO*‐vehicle and DKO*‐Amygdalin‐treated mice were harvested to quantify soluble cytokines according to the manufacturer's instructions (Mouse IL‐17A Quantikine ELISA Kit, Ref # M1700, mouse IL6 Quantikine ELISA Kit, Ref # M6000B, mouse G‐CSF Quantikine ELISA Kit, Ref # MCS00, R&D Systems).
Gago‐López N., Lagunas Arnal C., Perez J.J, & Wagner E.F. (2021). Topical application of an amygdalin analogue reduces inflammation and keratinocyte proliferation in a psoriasis mouse model. Experimental Dermatology, 30(11), 1662-1674.
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7

Quantification of G-CSF in RAW264.7 Cells

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The concentration of G-CSF in the culture medium from RAW264.7 cells was measured using a mouse G-CSF Quantikine ELISA kit (R & D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
Chang S.F., Li H.C., Huang Y.P., Tasi W.J., Chou Y.Y, & Lu S.C. (2016). SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3’ untranslated region in macrophages independently of its effect on p38 MAPK activity. Journal of Biomedical Science, 23, 3.
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8

Serum G-CSF Quantification Protocol

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Peripheral blood was collected in serum separation microvette tubes (SARSTEDT, Numbrecht, Germany http://www.sarstedt. com/php/main.php). Tubes were kept at room temperature for 2 hours, and then spun at 2,000g for 20 minutes as per manufacturers recommendation. Serum levels of G-CSF in treated mice were determined using a Mouse G-CSF Quantikine Elisa kit R&D systems, Minneapolis, MN http://www. rndsystems.com/.
Brooks R., Iyer S., Akada H., Neelam S., Russo C.M., Chisholm J.D, & Kerr W.G. (2015). Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi. Stem cells (Dayton, Ohio), 33(3).
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