Pd l1

Manufactured by Gene Tech

PD-L1 is a lab equipment product that functions as a protein. It is involved in the regulation of the immune system's response.

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Lab products found in correlation

Tissue microarray chip, by Shanghai Outdo Biotech Co. (1 mentions)

2 protocols using pd l1

Immunohistochemical Profiling of ccRCC

Cited 2 times

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Immunohistochemical staining was performed as described previously (26 (link)). Briefly, IHC was performed on the paraffin-embedded ccRCC tissues using Musashi-2 (Abcam, #ab76148), PD-L1 (GeneTech, #GT228002, Colone: ZR3), HIF1A (CST, (E1V6A), #48085), CA9 (MXB Biotechnologies, RAB-0615), KIT (MXB Biotechnologies, KIT-0029, Colone: YR145) and MKI67 (MXB Biotechnologies, MAB-0672, Colone: MX006) antibodies. IHC images were captured with a Leica Aperio Versa 200 microscope. Musashi-2 expression levels were scored by multiplying the percentage of positive cells by the staining intensity. The percentage was scored as follows: no positive cells, 0 point; 1%‐25%, 1 point; 26%‐50%, 2 points; 51%‐75%, 3 points; and 76%‐100%, 4 points. The staining intensity was scored as follows: no positive staining, 0 point; weak staining, 1 point; moderate staining, 2 points; and strong staining, 3 points. The final Musashi-2 IHC scores were classified as follows: 0‐4, low expression; and 6-12, high expression. For PD-L1 IHC evaluation, positive expression was defined as a tumor proportion score exceeding 1% (27 (link)).

Li H., Meng X., You X., Zhou W., Ouyang W., Pu X., Zhao R, & Tang H. (2022). Increased expression of the RNA-binding protein Musashi-2 is associated with immune infiltration and predicts better outcomes in ccRCC patients. Frontiers in Oncology, 12, 949705.

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Evaluating PD-L1, KLK6, and CSN1S1 in LUSC

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Tissue microarray chips containing 90 samples of LUSC and 90 samples of paired normal lung tissue were purchased from Outdo Biotech (Shanghai, China). Immunohistochemistry (IHC) staining was performed as previously described.^35,36 Microarray chips were stained with PD-L1 (Gene Tech), KLK6 (R&D) and CSN1S1 (Bioss). Quantitative analysis of the staining was independently assessed based on the percentage of positive cells and staining density by two experienced pathologists.³⁷ The final score was determined by adding the staining intensity score and the average proportion of positive cells score.

Xu F., Lin H., He P., He L., Chen J., Lin L, & Chen Y. (2020). A TP53-associated gene signature for prediction of prognosis and therapeutic responses in lung squamous cell carcinoma. Oncoimmunology, 9(1), 1731943.

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