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62 protocols using sodium hydroxide (naoh)

1

Phytochemical Analysis and Antioxidant Evaluation

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All the chemicals were of good grade and were purchased
from HiMedia
Leading BioScience Company. The chemicals and reagents, which are
used during the project work are as follows: sodium hydroxide, sodium
chloride, sodium acetate, methanol, petroleum ether, Folin–Ciocalteu
reagent, sodium hydroxide, sodium carbonate, sodium nitrite, and aluminum
chloride, which were obtained from Loba Chemie (Mumbai, India). Gallic
acid, quercetin, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained
from Sigma-Aldrich (St. Louis, Missouri, USA).
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2

Synthesis and Characterization of Metal Complexes

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All chemicals and regents used for this study were analytical grade and used without further purification. 1,10-phenanthroline monohydrate (BDH chemical Ltd., Poole, England), copper (II) acetate monohydrate [Cu(CO2CH3)2·H2O], triethylamine, NaHCO3, NaOH, and Mueller-Hinton agar were purchased from Loba Chemie PVT Ltd., Addis Ababa. Metformin hydrogen chloride (Met.HCl) and ciprofloxacin hydrogen chloride (Cip.HCl) were obtained from Cadila Pharmaceuticals PLC, Ethiopia. Methanol, ethanol, HCl, DMSO, ethyl acetate and dichloromethane (DCM) were purchased from Alpha Chemika, Addis Ababa, Ethiopia. Thin Layer Chromatography (MERCK Silica gel 60 F254) together with UV Cabinet (UV-Vis lamp at 254 and 365 nm) were used to monitor the progress of the chemical reactions. The melting points of the complexes were determined using capillary tubes (Thiele tube). UV-Vis spectrophotometer (SM-1600 Spectrophotometer), FTIR (Perkin-Elmer BX spectrometer, Shimadzu Corporation, Japan), TGA/DTC (DTG-60H SHIMADZU thermal analyzer) and High resolution mass spectra (Waters-LCT-Premier mass spectrometer) were used to characterize the synthesized metal complexes.
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3

Synthesis of Cellulose-Based Materials

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Different chemical compounds, such as nano-cellulose (Sigma-Aldrich, Darmstadt, Germany), Tylose (Methyl hydroxyethyl cellulose) (Sigma-Aldrich, Darmstadt, Germany), dimethyl sulfoxide (Sigma-Aldrich, Darmstadt, Germany), as well as NaOH, KOH and NaClO (Loba Chemie Pvt. Ltd., laboratory reagents & fine chemicals, Mumbai, India), were used.
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4

Antimicrobial Susceptibility Testing Protocol

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MIC was determined for specific reagents according to the American Society for Microbiology guidelines (Coyle et al., 2005 ). Stock solutions of Muller-Hinton broth (Fluka, Milwaukee, WI, USA) were prepared containing the following reagents: Tween 80 (5% v/v), SDS (5% w/v), KOH (3% w/v), NaOH (3% w/v), benzoic acid (0.3% w/v) (Lobachemie, Mumbai, India), EDTA (0.2% w/v) (Scharlau, Barcelona, Spain), and lactic acid (0.15% v/v) (WINLAB, East Midlands, England). Tenfold serial dilutions were prepared for each reagent. Aseptically, 100 µl of standard inoculum of S. Typhimurium (matched 0.5 McFarland standard) were added to each diluton. All the tubes were incubated for 24 h before tested for turbidity.
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5

Synthesis and Purification of α-AGL

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Xylose (99%) and benzyltributylammonium chloride (BTBAC, 99%) were procured from Spectrochem. PdCl2 was purchased from Sigma. NaBH4, 1,2-dichloroethane (DCE) (98%), HCl (aq., 35–38%), and tetrahydrofuran (THF) (99.5%) were obtained from Molychem. Sodium sulfate (anhydrous, 99%), NaOH, and deionized (DI) water were purchased from Loba Chemie Pvt. Ltd. Methanol (99.5%) and chloroform (98%) were purchased from Finar. AGL (>95% α-isomer) was purchased from Alfa Aesar and purified by passing through a plug of silica gel (60–120 mesh) using chloroform as the eluent. chloroform was then evaporated in a rotary evaporator under reduced pressure to obtain pure α-AGL as a faint-yellow oil. 2-Furanone was synthesized according to the literature procedure.14
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6

Synthesis of Zinc-Cobalt Ferrite Nanoparticles

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Zn0.4Co0.6Fe2O4 nanoparticles were prepared according to the previously reported procedure,14 except that zinc acetate was used instead of zinc nitrate. In this method, 1.616 g of Fe(NO3)3·9H2O (Loba Chemie Pvt Ltd, 98%), 0.1756 g of Zn(CH3COO)2·2H2O (Loba Chemie Pvt Ltd, 98%), and 0.349 g of Co(NO3)2·6H2O (Sigma-Aldrich, ≥98%) were dissolved in 40 mL of double distilled water (DDW) using a magnetic stirrer. Then, 1.28 g of NaOH (Loba Chemie Pvt Ltd, >98%) was dissolved in 10 mL of DDW and added drop-wise to the above solution. After that, the mixture was transferred to a 100 mL Teflon-lined autoclave and heated at 160 °C for 12 h in an electric furnace. The nanoparticles were collected using a permanent neodymium magnet (TM-30 × 50-N magnet, magnets4you Co., Germany), washed three times with DDW to remove inorganic byproducts, and twice with ethanol to remove organic byproducts. Finally, the nanoparticles were dried in an oven at 60 °C for 12 h under vacuum conditions.
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7

Titanium(III) Sulfate Mediated Reactions

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Titanium(III) sulfate (TS, Ti2(SO4)3, Fisher, Loughborough, Leicestershire, UK, 15%), sodium nitrate (NaNO3, Koch-light laboratories Ltd., Haverhill, Suffolk, UK, 98%), glycine (GLY, H2N-CH2-COOH, Sigma-Aldrich, St. Louis, MO, USA, 99%), sodium hydroxide (NaOH, LobaChemie, Pellets, Mumbai, India, 98%), MC-LR (Cal-Biochem, Nottingham, UK, 99%), and absolute ethanol (CH3CH2OH, Sigma-Aldrich, Darmstadt, Germany, 99.8%) were used without any further purification. The water used for the experiment was deionized water (DI, 8.2 µ Ω).
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8

Pharmacological Evaluation of Natural Compounds

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Pentylenetetrazol (PTZ) (Sigma Aldrich, Germany), gallic acid (Merck, Germany), Folin–Ciocalteu reagent (Loba Chemie, India), NaOH (Loba Chemie, India), AlCl3 (Loba Chemie, India), chloroform (Loba Chemie, India), HCl (BDH Laboratory Supplies, UK), citric acid (Avonchem, UK), Na2HPO4 (BDH Laboratory Supplies, UK), atropine (BDH Chemicals, UK), quercetin dihydrate (Sigma Aldrich, Germany), Tween80 (Loba Chemie, India), BCG (Sisco Chemical Laboratories, India), n-hexane (Loba Chemie, India), ethyl acetate (Trust Chemical Laboratories, UK), methanol (Sisco Research Laboratories, India), normal saline solution (Sansheng Pharmaceuticals, Ethiopia), potassium acetate (Blulux, India), sodium valproate (Sanofi, Spain), and phenytoin (Macleods Pharmaceuticals, India) were obtained from their respective vendors. All drugs and chemicals used were of analytical grade.
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9

Synthesis of Fe3O4 Nanoparticles

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Anhydrous FeCl 3 (96% purity) and FeSO 4 ·7H 2 O (99% purity) purchased from Merck Specialities Pvt Ltd., Mumbai, India and Loba Chemie Pvt Ltd., Mumbai, India, respectively, were used as precursors to synthesize Fe 3 O 4 nanoparticles. In addition, NaOH (98% purity) was used as a precipitating agent, which was procured from Loba Chemie Pvt Ltd., Mumbai, India. Distilled water was used for preparation of solutions, nanofluids, and washing purposes.
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10

Graphite Synthesis from Pencil Cores

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Na2SO4, 99% and Cd(CH3COO)2·2H2O, 99.5%, ethanol, HCl, HNO3, NaOH and Pb(CH3COO)2 were purchased from LOBA CHEMIE. Hp pencil cores were used as a source of graphite. All chemical reagents were pure and used without purification.
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