Choline oxidase

Manufactured by Avantor

Choline oxidase is an enzyme that catalyzes the oxidation of choline to betaine aldehyde and hydrogen peroxide. It is a commonly used enzyme in various analytical and diagnostic applications.

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Lab products found in correlation

Horseradish peroxidase, by Avantor (3 mentions) Tetracaine, by Merck Group (2 mentions) Hydroxychloroquine, by Cayman Chemical (2 mentions) 2 dioctanoyl sn glycero 3 phosphocholine c8 pc, by Cayman Chemical (2 mentions) Methyl beta cyclodextrin mβcd, by Merck Group (2 mentions) Valinomycin, by Bio-Techne (1 mentions) Amplex red 10 acetyl 3 7 dihydroxyphenoxazine, by Cayman Chemical (1 mentions) N dodecyl β d maltoside ddm, by Anatrace (1 mentions)

3 protocols using choline oxidase

Phospholipase D Activity Assay

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Hydroxychloroquine, PLD assay reagent amplex red 10-Acetyl-3,7-dihydroxyphenoxazine, and 2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC) were purchase from Cayman Chemical and tetracaine, methylbetacyclodextrin (MβCD), and cabbage PLD were purchased from Sigma–Aldrich. Horseradish peroxidase and choline oxidase were purchased from VWR.

Yuan Z., Pavel M.A., Wang H., Kwachukwu J.C., Mediouni S., Jablonski J.A., Nettles K.W., Reddy C.B., Valente S.T, & Hansen S.B. (2022). Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture. Communications Biology, 5, 958.

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Phospholipase D2 Activity Assay

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Hydroxychloroquine was purchase from Cayman Chemical and tetracaine was purchased from Sigma-Aldrich. Purified PLD2 from cabbage was purchased from Sigma-Aldrich respectively. PLD assay reagent amplex red 10-Acetyl-3,7-dihydroxyphenoxazine and 2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC) were purchased from Cayman Chemical. Horseradish peroxidase and choline oxidase were purchased from VWR. Methylbetacyclodextrin (MβCD) was purchased from Sigma-Aldrich.

Yuan Z., Pavel M.A., Wang H, & Hansen S.B. (2020). Hydroxychloroquine: mechanism of action inhibiting SARS-CoV2 entry.. bioRxiv.

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Lipid Substrate Assay for Phospholipase D

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Bacterial (Streptomyces chromofuscus), peanut, and cabbage PLD was purchased from Enzo and sigma-aldrich respectively. Amplex Red 10-Acetyl-3,7-dihydroxyphenoxazine (Cayman chemical). Horseradish peroxidase (VWR), choline oxidase (VWR). All lipids including 1,2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC), 1-palmitoyl-2-(dipyrrometheneboron difluoride) undecanoyl-sn-glycero-3-phosphocholine (TopFluorPC), 1-(dipyrrometheneboron difluoride) undecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (TopFluorLPC), 1 -palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC or 18:1 PC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol (POPEtOH), and 1,2-dioleoyl-sn-glycero-3-phosphoethanol (DOPEtOH) were purchased from Avanti Polar Lipids (Alabaster, AL) in chloroform at stock concentrations of 10 or 25 mg/mL except C8-PC and TopFluor lipids which were purchased as powder. Carbonyl cyanide m-chlorophenyl hydrazine (CCCP) was from Tocris Bioscience and made at a stock concentration of 1mM in ethanol. Valinomycin was also purchased from Tocris Bioscience and made at a stock concentration of 200μM in ethanol. The detergent n-dodecyl-β-D-maltoside (DDM) was purchased from Anatrace (D310S) and made at 200mM. ACMA (9-amino-6-chloro-2-methoxyacridine), was purchased from Life Technologies and made at a stock concentration of 2mM in ethanol.

Chung H.W., Petersen E.N., Cabanos C., Murphy K.R., Arif Pavel M., Hansen A.S., Ja W.W, & Hansen S.B. (2018). A Molecular Target for an Alcohol Chain Length Cutoff.. Journal of molecular biology, 431(2), 196-209.

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