Ab56417

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab56417 is a lab equipment product offered by Abcam. It is a device used for a specific function in a laboratory setting. The core function of this product is to perform a particular task required in scientific research and analysis. No further details or interpretation about the intended use of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Ab22510, by Abcam (1 mentions) Ab68672, by Abcam (1 mentions) Ab216327, by Abcam (1 mentions) Bond max automated ihc ish stainer, by Leica (1 mentions) Ncl l cd4 368, by Leica (1 mentions) Ncl l cd8 4b11, by Leica (1 mentions) Sc 100289, by Santa Cruz Biotechnology (1 mentions) Mca1477, by Bio-Rad (1 mentions) Bond polymer refine detection kit, by Leica (1 mentions) Occludin, by Thermo Fisher Scientific (1 mentions) Ab8226, by Abcam (1 mentions) Chemidoc xrs imager, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Rela p65, by Cell Signaling Technology (1 mentions) L1cam, by Merck Group (1 mentions) Ki 67p, by Dianova (1 mentions) Ventana benchmark xt, by Roche (1 mentions) Olig2, by R&D Systems (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Ab53156, by Abcam (1 mentions)

4 protocols using ab56417

Comprehensive Immunohistochemical Profiling of Tissues

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Paraffin blocks were cut into 1 µm-thick sections and fixed onto a poly-L-lysine treated glass slide and then used for histological and immunohistochemical analyses. All sections were stained with H&E following manufacturer’s protocol to achieve correct orientation of the biopsy. Immunohistochemical staining was performed using Bond Polymer refine Detection kit on a BOND-MAX Automated IHC/ISH Stainer (Leica Biosystems) following the manufacturer’s protocol. The following antibodies were used: mouse monoclonal anti-human CD19 (NCL-L-CD19-163, Leica Biosystems), rat anti-human CD3 (MCA1477,Bio-Rad), mouse monoclonal anti-human CD4 (NCL-L-CD4-368, Leica Biosystems), mouse monoclonal anti-human CD8 (NCL-L-CD8-4B11, Leica Biosystems), mouse monoclonal anti-human Claudin-1 (ab56417, Abcam), mouse monoclonal anti-human E-Cadherin (36B5) (PA0387, Leica Biosystems), mouse monoclonal anti-human FoxP3 (ab22510, Abcam), mouse monoclonal anti-human γδ-TCR (sc-100289, Santa Cruz Biotechnology), mouse monoclonal anti-human Langerin (ab49730, Abcam), rabbit recombinant monoclonal [EPR20992] to Occludin (ab216327, Abcam), and rabbit polyclonal to Neutrophil Elastase (ab68672, Abcam). Positive and negative controls were included for each experiment.

Sanchez-Solares J., Sanchez L., Pablo-Torres C., Diaz-Fernandez C., Sørensen P., Barber D, & Gomez-Casado C. (2021). Celiac Disease Causes Epithelial Disruption and Regulatory T Cell Recruitment in the Oral Mucosa. Frontiers in Immunology, 12, 623805.

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Quantifying Tight Junction Protein Expression

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Sample protein concentrations were determined by Bradford assay. Membranes were blocked and incubated with primary antibody overnight (β-actin: 1 mg/ml, ab8226, Abcam, Cambridge, MA, USA; Claudin-1: 0.5 mg/ml, ab56417, Abcam, Cambridge, MA, USA; Claudin-5: 0.5 mg/mL, 35–2500, Thermo Fisher Scientific; Occludin: 0.5 mg/mL, 71–1500, Thermo Fisher Scientific). Membranes were washed and incubated with horseradish peroxidase-conjugated ImmunoPure™ secondary IgG (1:10,000; Pierce, Rockford, IL, USA) for 1 h at room temperature. Protein bands were visualized using Pierce SuperSignal™ West Pico and West Femto chemiluminescent substrates (Thermo Fisher Scientific) using a ChemiDoc™ XRS imager (Bio-Rad Laboratories, Hercules, CA, USA). Optical density was measured with ImageLab software (v.4.6.9; Bio-Rad Laboratories). Four replicate blots were obtained from pooled tissue of the seven mice in each group.

Yanckello L.M., Hoffman J.D., Chang Y.H., Lin P., Nehra G., Chlipala G., McCulloch S.D., Hammond T.C., Yackzan A.T., Lane A.N., Green S.J., Hartz A.M, & Lin A.L. (2021). Apolipoprotein E genotype-dependent nutrigenetic effects to prebiotic inulin for modulating systemic metabolism and neuroprotection in mice via gut-brain axis. Nutritional neuroscience, 25(8), 1669-1679.

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Comprehensive Immunohistochemical Profiling

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Immunohistochemical (IHC) analysis was performed using standard protocols on an automated Ventana Benchmark XT immunostaining system (Roche‐Ventana, Darmstadt, Germany). We used primary antibodies against glial fibrillary acidic protein (GFAP; rabbit polyclonal, Agilent/Dako, Glostrup, Denmark), microtubule‐associated protein 2 (Map2; mouse monoclonal (HM‐2), Sigma‐Aldrich, St. Louis, MO, USA), p53 protein (mouse monoclonal (DO‐7), Agilent/Dako, Glostrup, Denmark), Olig‐2 (goat polyclonal, R&D Systems, Abingdon, UK), epithelial membrane antigen (mouse monoclonal (E‐29), Agilent/Dako, Glostrup, Denmark), p65 RelA (rabbit monoclonal (D14E12), Cell signaling, Danvers, U.S.A.), L1CAM (mouse monoclonal, (UJ127.11), Sigma–Aldrich, St Louis, MO, USA), Claudin‐1 (mouse monoclonal (ab56417), Abcam, Cambridge, UK), Ki67 (mouse monoclonal (Ki‐67P), Dianova, Hamburg, Germany), phospho‐histone‐3 (rabbit polyclonal, Bioclare Medical, Hague, Netherlands) and NF (mouse monoclonal (2F11), Agilent/Dako).

Andreiuolo F., Varlet P., Tauziède‐Espariat A., Jünger S.T., Dörner E., Dreschmann V., Kuchelmeister K., Waha A., Haberler C., Slavc I., Corbacioglu S., Riemenschneider M.J., Leipold A., Rüdiger T., Körholz D., Acker T., Russo A., Faber J., Sommer C., Armbrust S., Rose M., Erdlenbruch B., Hans V.H., Bernbeck B., Schneider D., Lorenzen J., Ebinger M., Handgretinger R., Neumann M., van Buiren M., Prinz M., Roganovic J., Jakovcevic A., Park S., Grill J., Puget S., Messing‐Jünger M., Reinhard H., Bergmann M., Hattingen E, & Pietsch T. (2018). Childhood supratentorial ependymomas with YAP1‐MAMLD1 fusion: an entity with characteristic clinical, radiological, cytogenetic and histopathological features. Brain Pathology, 29(2), 205-216.

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Colon Tight Junction Protein IHC

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Immunohistochemistry (IHC) was performed on FFPE tissue using the following primary antibodies: claudin-1 (Abcam, ab56417, 1:250), claudin-2 (Abcam, ab125293, 1:75), claudin-3 (GeneTex, GTX15102, 1:50), claudin-4 (Abcam, ab53156, 1:1,000), and claudin-8 (Abcam, ab183738, 1:1,000) followed by DAKO EnVision Detection System Kit or R&D System Kit (Supplementary Figure S1). Whole slide for each human sample/animal sample was evaluated. Human samples were at least 1 cm long. Animal samples represented the whole colon. Colon was sampled as proximal and distal parts; each part was cut into three to four pieces; whole material was embedded. On one slide, there have been three to four transversal cuts from proximal colon and three to four cuts from the distal colon.

Čužić S., Antolić M., Ognjenović A., Stupin-Polančec D., Petrinić Grba A., Hrvačić B., Dominis Kramarić M., Musladin S., Požgaj L., Zlatar I., Polančec D., Aralica G., Banić M., Urek M., Mijandrušić Sinčić B., Čubranić A., Glojnarić I., Bosnar M, & Eraković Haber V. (2021). Claudins: Beyond Tight Junctions in Human IBD and Murine Models. Frontiers in Pharmacology, 12, 682614.

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