The concentrations of matrix metalloproteinase (MMP)-1 (Rat MMP-1 ELISA Kit, E-EL-R0617c), MMP-3 (Rat MMP-3 ELISA Kit, E-EL-R0619c), IL-6 (Rat IL-6 ELISA Kit, E-EL-R0015c), IL-1β (Rat IL-1β ELISA Kit, E-EL-R0012c), and TNF-α (Rat TNF-α ELISA Kit, E-EL-R2856c) in serum were detected using commercial ELISA kits from Elabscience Biotechnology Co. (China), following the manufacturer’s instructions.
Rat mmp 3 elisa kit
Manufactured by Elabscience
Sourced in China
The Rat MMP-3 ELISA Kit is a quantitative assay designed to measure the level of rat matrix metalloproteinase-3 (MMP-3) in various sample types. It utilizes the sandwich enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify rat MMP-3 in the sample.
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2 protocols using rat mmp 3 elisa kit
1
Serum Biomarker Profiling in Rats
2
Quantifying Inflammatory and Matrix Biomarkers
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After grouping, the supernatants of each cell culture were collected by centrifugation, and then the ELISA kits were used (Rat IL-6 ELISA Kit was purchased from US ImmunoWay Corporation, Rat MMP-3 ELISA Kit, Rat MMP-13 ELISA Kit, Rat TGF-β ELISA Kit was purchased from Elabscience Biotechnology Co. Ltd).
The expression levels of IL-6, TGF-β, MMP-3 and MMP-13 in the cell culture supernatant were measured.
Samples were incubated at 37°C for 1 hour. After washing the plates, the biotinylated antibodies were added, and the plates were incubated at 37°C for 1 hour. The plates were washed, the enzyme conjugate was added, and the sample was incubated at 37°C for 30 min. Then, the plate was washed and patted dry, the TBS substrate solution was added, and the samples were incubated at 37°C in light for 10-15 min after the termination solution was added to stop the reaction. The absorbance was measured using a microplate reader at 450 nm, and the results were calculated.
The expression levels of IL-6, TGF-β, MMP-3 and MMP-13 in the cell culture supernatant were measured.
Samples were incubated at 37°C for 1 hour. After washing the plates, the biotinylated antibodies were added, and the plates were incubated at 37°C for 1 hour. The plates were washed, the enzyme conjugate was added, and the sample was incubated at 37°C for 30 min. Then, the plate was washed and patted dry, the TBS substrate solution was added, and the samples were incubated at 37°C in light for 10-15 min after the termination solution was added to stop the reaction. The absorbance was measured using a microplate reader at 450 nm, and the results were calculated.
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