Screenfect sirna transfection kit

Cell lines and primary neurons were transfected with the respective synthesized 21-mer siRNAs with dTdT using the ScreenFect siRNA transfection kit (Fujifilm) in accordance with the manufacturer’s instructions. The medium was replaced 4 h after transfection and generally used for ≥48 h after transfection for biochemical experiments. Under these conditions, attached cells incorporating trypan blue were estimated to be less than 5% in each experiment 48 h after transfection.

Cells were transfected with the respective synthesized 21-mer siRNAs with dTdT (Fasmac, Kanagawa, Japan) using the ScreenFect siRNA transfection kit (FUJIFILM, Tokyo, Japan), according to the manufacturer’s instructions. The medium was replaced 4 h after transfection and cells were generally used for experiments 48 h after transfection.
Cells were also transfected with the respective plasmids (pEF1a (pEF-BOS modified type) encoding CasRx and pSINmU6 encoding gRNA or pcDNA3.1-N-EGFP-human ACE2 intracellular domain) using the ScreenFect A transfection kit (FUJIFILM, Tokyo, Japan), according to the manufacturer’s instructions. The medium was replaced 4 h after transfection and cells were generally used for experiments 48 h after transfection. All key reagents and antibodies used are listed in Table 1.

Cells were transfected with the respective siRNAs using a ScreenFect siRNA transfection kit (FujiFilm), in accordance with the manufacturer’s instructions. The medium was replaced 4 h after transfection and was generally used for experiments 48 h after transfection. The siRNA target sequences for mouse PPP1CC, mouse PPP2CA, and control luciferase with no significant homology to any mammalian gene sequences were described in Table 2. Attached, trypan incorporating cells were less than 5% in each experiment.