Experiments were performed in white, 384-well microtiter plates (Corning 3572 or Greiner 781207). TR-FRET measurements were acquired on a Tecan SPARK plate reader with SPARKCONTROL software version V2.1 (Tecan Group Ltd.), with the following settings adapted from our previous TR-FRET experiments on other proteins32 (link): 340/50 nm excitation, 490/10 nm (Tb) and 520/10 nm (FITC) emission, 100 μs delay, 400 μs integration. The 490/10 and 520/10 emission channels were acquired with a dichroic 510 mirror, using independently optimized detector gain settings. The TR-FRET ratio was taken as the 520/490 nm intensity ratio on a per-well basis.
Sparkcontrol software version v2
Manufactured by Tecan
SPARKCONTROL software version V2.1 is a laboratory equipment management software tool. It provides basic functionalities for controlling and monitoring various laboratory instruments.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using sparkcontrol software version v2
1
TR-FRET Assay Protocol for Protein Analysis
2
Time-Resolved FRET Assay Development
3
Time-Resolved FRET Assay Optimization
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Unless otherwise noted, experiments were performed in white, 384-well microtiter plates (Corning 3572) in 30 μL assay volume. TR-FRET measurements were acquired on a Tecan SPARK plate reader with SPARKCONTROL software version V2.1 (Tecan Group Ltd.), with the following settings: 340/50 nm excitation, 490/10 nm (Tb), and 520/10 nm (AF488) emission, 100 μs delay, 400 μs integration. The 490/10 nm and 520/10 nm emission channels were acquired with a 50% mirror and a dichroic 510 mirror, respectively, using independently optimized detector gain settings unless specified otherwise. The TR-FRET ratio was taken as the 520/490 nm intensity ratio on a per-well basis.
4
Time-Resolved FRET Assay Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless otherwise noted, experiments were performed in white, 384-well microtiter plates (Corning 3572 or PerkinElmer ProxiPlate-384 Plus) in 30 μL or 10 μL assay volume, respectively. TR-FRET measurements were acquired on a Tecan SPARK plate reader with SPARKCONTROL software version V2.1 (Tecan Group Ltd.), with the following settings: 340/50 nm excitation, 490/10 nm (Tb), 520/10 nm (FITC) emission, 100 μs delay, and 400 μs integration. The 490/10 and 520/10 nm emission channels were acquired with a 50% mirror and a dichroic 510 mirror, respectively, using independently optimized detector gain settings unless specified otherwise. The TR-FRET ratio was taken as the 520/490 nm intensity ratio on a per-well basis.
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