Anti lat1

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-LAT1 is a primary antibody that targets the LAT1 protein. LAT1 is a neutral amino acid transporter that facilitates the uptake of essential amino acids into cells. This antibody can be used to detect and analyze the expression of the LAT1 protein.

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Lab products found in correlation

4 protocols using anti lat1

Western Blot Antibody Reagents

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Anti-S6K (#9202), anti-phospho S6K Thr389 (#9234), anti-AKT (#9272), anti-phospho AKT Ser473 (#9271), anti-LAT1 (#5347), anti-mTOR (#2983), anti-Raptor (#2280), anti-Flag (#14793), and anti-Rictor (#2140) were purchased from Cell Signaling Technology. Anti-tubulin (05-829) was purchased from Millipore. Anti-β-actin (A700-057) and anti-Flag M2 (F1804) were purchased from Sigma. HA-horseradish peroxidase (HRP) (11-814-150-001) was purchased from Roche. Anti-HA (sc-7392) was purchased from Santa Cruz Biotechnology. Anti-LAMP2 (ab25631) and anti-sodium potassium ATPase (ab76020) were purchased from Abcam.

Tae K., Kim S.J., Cho S.W., Lee H., Cha H.S, & Choi C.Y. (2023). L-Type Amino Acid Transporter 1 (LAT1) Promotes PMA-Induced Cell Migration through mTORC2 Activation at the Lysosome. Cells, 12(20), 2504.

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Antibody Validation for Protein Analysis

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Anti-LAT1 (#5347), anti-4F2hc (#47213), anti-xCT/SLC7A11 (#12691) and anti-HA (#3724) were purchased from Cell Signaling. Anti-SCRIB (C-20), anti-SCRIB (C-2), anti-LLGL2 antibody (A-4), anti-CD98 (4F2), anti-CD98 (E-5), anti-Erk2 (C-14), and anti-cyclin D1 (H-295) were purchased from Santa Cruz. Anti-HA, anti-CD98hc (BMP090), anti-SLC7A5 (BMP011), and anti-DDDDK-tag (M185) antibodies were purchased from MBL. Anti-SLC7A5 (HPA052673) and anti-SCRIB (HPA023557) antibodies were purchased from ATLAS ANTIBODIES. Anti-Flag antibody (M2) was purchased from Sigma.

Saito Y., Matsuda S., Ohnishi N., Endo K., Ashitani S., Ohishi M., Ueno A., Tomita M., Ueda K., Soga T, & Muthuswamy S.K. (2022). Polarity protein SCRIB interacts with SLC3A2 to regulate proliferation and tamoxifen resistance in ER+ breast cancer. Communications Biology, 5, 403.

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Profiling Luminal ER-positive Breast Cancer

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Luminal estrogen-receptor (ER)-positive breast cancer (BC) cell lines (MCF7, T47D, ZR-75-1) were acquired from the American Type Culture Collection (Manassas, VA, USA) and cultured at 37 °C with 5% CO₂. MCF7 was cultured in DMEM/F12 with 10% fetal bovine serum (FBS) and Penicillin-Streptomycin (P/S). T47D and ZR-75-1 were cultured in RPMI-1640 with 10% FBS and P/S. Media, P/S and FBS were purchased from Thermo Fisher Scientific (Waltham, MA, USA). 2-Amino-2-norbornanecarboxylic acid (BCH), JPH203 (KYT-0353) and Dimethyl sulfoxide (DMSO) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Stock BCH (100 mM) was prepared in media. Stock JPH-203 (1 mg/mL; 2.1 mM) was prepared in DMSO. Antibodies were acquired from Cell Signaling Technology (Danvers, MA, USA): anti-LAT1 (rabbit), anti-CD98 (rabbit), anti-S6 Ribosomal Protein (rabbit), β-Actin (rabbit), anti-Phospho-S6 Ribosomal Protein (Ser^235/236).

Thompson C., Rahman M.M., Singh S., Arthur S., Sierra-Bakhshi C., Russell R., Denning K., Sundaram U, & Salisbury T. (2021). The Adipose Tissue-Derived Secretome (ADS) in Obesity Uniquely Induces L-Type Amino Acid Transporter 1 (LAT1) and mTOR Signaling in Estrogen-Receptor-Positive Breast Cancer Cells. International Journal of Molecular Sciences, 22(13), 6706.

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Analyzing MET and Akt Phosphorylation Pathways

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Cells were subjected to starvation treatment (cultured in RPMI 1640 medium without addition of FBS) for 24 h, and then phosphorylation stimulation was performed. Hepatocyte growth factor (HGF) was purchased from PeproTech (Shanghai, China) as a stimulus for MET phosphorylation. Phosphorylation stimulation with HGF was performed at 40 ng/mL for 10 min. For Akt phosphorylation, cells were plated in medium supplemented with 10% FBS for 10 min. Antibodies anti-Akt (pan, rabbit IgG, cat no. #4691), phospho-Akt (rabbit IgG, Ser 473, #4060), anti-MET (rabbit IgG, #8198), phospho-MET (rabbit IgG, Tyr 1234/1235, #3077), anti-SLC1A5 (#4692), and anti-LAT1 (#5347) were purchased from Cell Signaling Technology (Danvers, MA, USA), and GAPDH (sc-32233) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Sato T., Kawasaki Y., Maekawa M., Takasaki S., Morozumi K., Sato M., Shimada S., Kawamorita N., Yamashita S., Mitsuzuka K., Mano N, & Ito A. (2020). Metabolomic Analysis to Elucidate Mechanisms of Sunitinib Resistance in Renal Cell Carcinoma. Metabolites, 11(1), 1.

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