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Nunc lab tektm 2

Manufactured by Thermo Fisher Scientific
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The NuncTM Lab-TekTM II is a multi-well chamber slide system designed for cell culture and microscopy applications. It provides a convenient and controlled environment for growing and observing cells.

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Lab products found in correlation

Matrigel, by BD (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Everbrite mounting medium, by Biotium (1 mentions) Raw264, by American Type Culture Collection (1 mentions) Raw264, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Lab-Tek (191 citations) Lab-Tek II (175 citations) Nunc Lab-Tek (110 citations) Nunc Lab-Tek II (60 citations) NuncTM (46 citations) NuncTM Lab-TekTM (4 citations) Nunc Lab-Tek II Chambered Coverglass slides (3 citations) NuncTM Lab-TekTM II Chambered Coverglass (2 citations) NuncTM Lab-TekTM II Chamber SlideTM System (2 citations) Nunc Lab-TekTM II chamber slides (2 citations)

3 protocols using nunc lab tektm 2

1

3D Cell Culture in Matrigel

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Four thousand cells were cultured in growth factor–reduced reconstituted basement membrane (Matrigel; BD Biosciences) in an eight-well NuncTM Lab-TekTM II chamber slide (Thermo-Fisher Scientific, NY) as described previously (Debnath et al., 2003 (link)). Cells were grown in the presence of or without 2 μg/ml dox in 37°C for 10 days. The cell lines were assayed in three independent experiments.
Shen H., Chen Y., Wan Y., Liu T., Wang J., Zhang Y., Wei L., Hu Q., Xu B., Chernov M., Frangou C, & Zhang J. (2021). Identification of TAZ-Dependent Breast Cancer Vulnerabilities Using a Chemical Genomics Screening Approach. Frontiers in Cell and Developmental Biology, 9, 673374.
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2

Visualizing Intracellular Trafficking of dsRNA

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Since CypHer5E has a fluorescence maximum around pH 5 and almost no fluorescence around pH 7, it can indicate localization within the late endosomes.35 (link) One day prior to the experiment, 1.2 × 105 cells/well were seeded in eight well chamber microscope slides (NuncTM Lab-TekTM II, ThermoFisher Scientific, Waltham, MA). The next day, 25 ng of CypHer5E-dsGFP, either naked or conjugated with PLR10 (PLR:dsRNA mass ratio of 1:1), c-PLR10/Au/BSA and c-PLR10/Au/Hyd NPs (NP:dsRNA mass ratio of 5:1) and washed one time with DI water to remove free CypHer5E-dsGFP were mixed with 100 μL of SF900 II SFM medium then added to cells. At 4 h after exposure, the cells were washed twice with 1X phosphate-buffered saline (PBS), fixed with 100 μL of 4% paraformaldehyde for 20 min at room temperature and stained with EverBrite mounting medium (Biotium, Fremont, CA) containing 4′,6-diamidino-2-phenylindole (DAPI). The cells were visualized with Leica SP8 confocal microscope at 63× magnification by overlapping DAPI (Blue; for nuclei), Alexa 633 (Red; for CypHer5E labeled dsRNA), and bright-field (BF) filters images.
Laisney J., Gurusamy D., Baddar Z.E., Palli S.R, & Unrine J.M. (2020). RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles. ACS applied materials & interfaces, 12(23), 25645-25657.
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3

Microscopic Analysis of RAW264.7 Cells

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The murine MΦ cell line RAW264.7 was purchased from ATCC (Manassas, VA) and maintained in growth media (DMEM (Life Technologies, Carlsbad, CA) supplemented with 10% FBS and 1% penicillin/streptomycin). Isolated CLDIs were added at 20 μM solution equivalent concentration in growth media to 8-well chamber-slides (NuncTM LabtekTM II, Thermo-Scientific Catalogue No. 154534) containing 5 × 104 RAW264.7 cells/well and incubated (24 h at 37 °C and 5% CO2). Following incubation, cells were fixed using 4% paraformaldehyde in PBS for 15 mins at room temperature followed by rinsing and washing with ice-cold PBS twice. The samples were counterstained with Hoechst33342 for nuclear labelling followed by removal of chambers. A drop of Prolong Gold® (Life Technologies) was placed on the sample and covered with a glass coverslip for imaging.
Keswani R.K., Tian C., Peryea T., Girish G., Wang X, & Rosania G.R. (2016). Repositioning Clofazimine as a Macrophage-Targeting Photoacoustic Contrast Agent. Scientific Reports, 6, 23528.
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