Cd23 b3b4

Manufactured by BioLegend

Sourced in United States

CD23 (B3B4) is a cell surface marker that is expressed on B cells. It plays a role in IgE-mediated regulation of the immune response.

Automatically generated - may contain errors

Lab products found in correlation

Cd21 cd35 7e9, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions) Cd3 145 2c11, by BD (1 mentions) Cd11b m1 70, by BD (1 mentions) Cd19 1d3, by BD (1 mentions) Cd21 7g6, by BD (1 mentions) Igm 2 41, by BD (1 mentions) Cd93 aa4 1, by BD (1 mentions) Ammonium chloride potassium lysing buffer, by Lonza (1 mentions) B220 ra3 6b2, by BioLegend (1 mentions) Cd93 aa4, by BioLegend (1 mentions) Cd24 m1 69, by BioLegend (1 mentions) Cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Cd80 16 10a1, by BioLegend (1 mentions) Cd86 gl 1, by BioLegend (1 mentions) Cd69 h1.2f3, by BioLegend (1 mentions) Cd44 im7, by BioLegend (1 mentions) Facscanto 10c, by BD (1 mentions) Cd3e 145 2c11, by Thermo Fisher Scientific (1 mentions) Cd4 gk1, by Thermo Fisher Scientific (1 mentions)

6 protocols using cd23 b3b4

Mouse Spleen Cell Isolation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight-week-old C57BL/6 mice were used for analysis. Cells from total spleen were dispersed by mechanical force. Resulting single-cell suspension was filtered through a 70-mm nylon mesh. Red blood cells were lysed using Ammonium-Chloride-Potassium lysing buffer (Lonza) at 1 ml per spleen for an incubation of 1 min on ice. In all subsequent steps, cells were incubated in 1× PBS containing 1% bovine serum albumin at 4°C and processed similar to the human cells as described above with the following exceptions. Fc block was anti-mouse (BD Biosciences). Primary antibody panel was as follows: CD3 (145–2C11, BD Biosciences), CD19 (1D3, BD Biosciences), CD11b (M1/70, BD Biosciences), CD21 (7G6, BD Biosciences), CD45R (RA3–6B2, Invitrogen), IgM (II/41, BD Biosciences), CD23 (B3B4, BioLegend), IgD (11026C.2a, BioLegend), and CD93 (AA4–1, BD Biosciences). A subset of experiments required the addition of CD39 (DUHA59) and CD73 (TY/118, BioLegend) to this panel.

Farmer J.R., Allard-Chamard H., Sun N., Ahmad M., Bertocchi A., Mahajan V.S., Aicher T., Arnold J., Benson M.D., Morningstar J., Barmettler S., Yuen G., Murphy S.J., Walter J.E., Ghebremichael M., Shalek A.K., Batista F., Gerszten R, & Pillai S. (2019). Induction of metabolic quiescence defines the transitional to follicular B cell switch. Science signaling, 12(604), eaaw5573.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions from mouse liver, BM (one femur and tibia), spleen, thymus, mesenteric lymph nodes, and peritoneal cavity were processed as previously described (47 (link)), and data were acquired on a FACSCanto10c (BD) and analyzed with FlowJo Software (Tree Star).
The following anti-mouse antibodies were used: B220 (RA3-6B2, BioLegend), BAFFR (7H22-E16,BD), BP-1 (6C3, BioLegend), CD3e (145-2C11, eBioscience), CD4 (GK1.5, eBioscience), CD5 (53-7.3, BioLegend), CD8 (53-6.7, BioLegend), CD9 (MZ3, BioLegend), CD11b (M1/70, eBioscience), CD19 (6D5, 1D3, BioLegend), CD21/CD35 (7E9, BioLegend), CD23 (B3B4, BioLegend), CD24 (M1/69, BioLegend), CD25 (PC61, Biolgend), CD43 (S7, BioLegend), CD44 (IM7, BioLegend), CD45.1 (A20, BioLegend), CD45.2 (104, BioLegend), CD69 (H1.2F3, BioLegend), CD80 (16-10A1, BioLegend), CD86 (GL1, BioLegend), CD93 (AA4.1, BioLegend), DAPI (BIOTIUM), Gr-1 (RB6-8C5, eBioscience), IgDa (AMS-9.1, BioLegend), IgD (11-26c, BioLegend), IgMa (DS-1, MA-69, BioLegend), IgM (Il/41, RMM-1, BioLegend), TCRγδ (GL3, BioLegend), Live dead dye (Zombie Aqua Dye, BioLegend), Live dead dye (Zombie NIR, BioLegend), pERK (4B11B69, BD), pPLCγ2 (K86-1161, BD), Lin28b (AP1485C, ABGENT), and anti-rabbit IgG (BioLegend).

Xu X., Deobagkar-Lele M., Bull K.R., Crockford T.L., Mead A.J., Cribbs A.P., Sims D., Anzilotti C, & Cornall R.J. (2020). An ontogenetic switch drives the positive and negative selection of B cells. Proceedings of the National Academy of Sciences of the United States of America, 117(7), 3718-3727.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of Mouse B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions were stained with mAb against mouse B220 (RA3-6B2; BD Pharmingen; 1 : 200), IgM (331.12; 1 : 800), IgD (1126 C; 1 : 800), Gr-1 (RB6-8C5; 1 : 800), CD21 (7G6; 1 : 2000), CD23 (B3B4; Biolegend; 1 : 200), IgG1 (X56; BD Pharmingen; 1 : 300), IgG3 (pooled antisera; Southern Biotech; 1 : 250), CD138 (281-2; BD Pharmingen; 1 : 600), FcgR (2.4G2; 1 : 10), BAFF-R (7H22-E16; BD Biosciences; 1 : 100), CD40 (11-0402-86; eBioscience; 1 : 200) and CD98 (RL388; Biolegend; 1 :500). Cells were analyzed on FACSCanto flow cytometers and cell sorting was carried out using FACSDiVa or Aria flow cytometers (BD Biosciences). Intracellular staining for BCL6 (7D1) was performed using the FoxP3 staining buffer set (eBiosciences). All Abs were produced in-house unless otherwise indicated. Antigen-specific B cells were identified by binding NP coupled to phycoerythrin.

Willis S.N., Tellier J., Liao Y., Trezise S., Light A., O’Donnell K., Garrett-Sinha L.A., Shi W., Tarlinton D.M, & Nutt S.L. (2017). Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB. Nature Communications, 8, 1426.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following mAbs against mouse IgG1 (A85-1), IgG2b (RMG2b1), IgG2a^b (5.7), IgG3 (R40-82), IgM^b (AF6-78), CD38 (90), CD45R/B220 (RA3-6B2), CD95/Fas (Jo2), CD35 (8C12), CD11b (M1/70), CD11c (HL3), and CD8 (53-6.7) were purchased from BD Biosciences. Antimouse CD45.1 (A20), CD45.2 (104), CD3e (eBio500A2) were obtained from eBioscience. The mAbs against murine CD93/C1qR (AA4.1), CD21/35 (7E9), and CD23 (B3B4) were obtained from BioLegend, and anti-mouse BAFFR (9B9) was purchased from AdipoGen Life Sciences. Streptavidin (SA)-PE, -BV421, and -BUV395 were purchased from BD Biosciences. Purified antimouse CD16/32 (2.4G2) and HyHEL9 mAbs were sourced from the University of California, San Francisco Hybridoma Core, and the latter was conjugated to Alexa Fluor 647 using the Alexa Fluor 647 Protein Labeling Kit (Invitrogen) according to the manufacturer’s instructions. Purified anti-CD38 was conjugated to Pacific Orange Antibody Labeling Kit (Invitrogen) according to the manufacturer’s instructions. Staining for BrdU incorporation was performed using the FITC BrdU Flow kit (BD Biosciences) according to the manufacturer’s instructions.

Lau A.W., Turner V.M., Bourne K., Hermes J.R., Chan T.D, & Brink R. (2020). BAFFR controls early memory B cell responses but is dispensable for germinal center function. The Journal of Experimental Medicine, 218(2), e20191167.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cells were resuspended in PBS, 2% FBS, and stained with fluorochrome-conjugated or biotinylated antibodies against B220 (RA3-6B2), IgD (11-26c-2a), IgM (R6-60.2), CD1d (K253), CD24 (M1/69), CD4 (GK1.5), CD8 (53-6.7), CD11c (HL3), CD11b (M1/70), CD19 (1D3), Notch-2 (16F11), CD93 (AA4.1), BP-1 (6C3), CD21/35 (4E3), CD23 (B3B4), CD43 (S7), and ADAM10 (all from Biolegend, BD Pharmingen, Thermo Fisher Scientific or R&D Systems). Biotin-labeled antibodies were visualized with fluorochrome-conjugated streptavidin (Thermo Fisher Scientific). LIVE/DEAD^® Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific) was used in all experiments to exclude dead cells. Compensation was performed using CompBeads (BD Biosciences) and ArC™ Amine Reactive Compensation Bead individually stained with each fluorochrome. Compensation matrices were calculated with FACSdiva software. Data acquisitions were done on FACSCanto II (BD) flow cytometer and analyzed with FlowJo software version 9 (Treestar).

Hwang I.Y., Boularan C., Harrison K, & Kehrl J.H. (2018). Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Frontiers in Immunology, 9, 687.

+ Open protocol

+ Expand

Single-cell Flow Cytometry Analysis of Immune Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

A single-cell suspension was prepared using the Tumor Dissociation Kit according to the manufacturer's protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were further treated by Ammonium-Chloride-Potassium (ACK) Lysing Buffer, and lysates were analyzed by the FACSCanto II (BD Bioscience, USA).
Antibodies used in FACS analysis were as follows: B220 (RA3-6B2), TCRβ (H57-597), CD21/CD35 (7E9), and CD23 (B3B4) from Biolegend; IgM (R6-60.2) from BD Biosciences. Single-cell suspensions from the bone marrow and the spleen were blocked with 10 µg/ml of anti-mouse CD16/CD32 (2.4G2; BD Biosciences) and then stained with fluorochrome-conjugated antibodies along with 7-aminoactinomycin D (7-AAD; Sigma) to exclude dead cells. Data were acquired on a FACSCanto II (BD Biosciences) and analyzed with BD FACSDiva software (BD Biosciences).

Tran Nguyen Truc L., Matsuda S., Takenouchi A., Tran Thuy Huong Q., Kotani Y., Miyazaki T., Kanda H., Yoshizawa K, & Tsukaguchi H. (2023). Mechanism of cystogenesis by Cd79a-driven, conditional mTOR activation in developing mouse nephrons. Scientific Reports, 13, 508.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!