Gtx100863

Manufactured by GeneTex

The GTX100863 is a laboratory instrument designed for DNA sequencing. It utilizes advanced technology to accurately and efficiently analyze genetic samples. The core function of this product is to provide researchers with a reliable tool for genetic analysis and investigation.

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Lab products found in correlation

Mab374, by Santa Cruz Biotechnology (1 mentions) Sc 5286, by Santa Cruz Biotechnology (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Merck Group (1 mentions) Nanog, by GeneTex (1 mentions) Krt19, by Thermo Fisher Scientific (1 mentions)

2 protocols using gtx100863

Antibodies for Pluripotency Protein Detection

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Antibodies for immunoblotting were as follows: Oct4, Santa Cruz sc-5279; Oct1, Bethyl A301-716A + A301–171A; Nanog, GeneTex GTX100863; Sox2, GeneTex GTX101507; GAPDH, EMD Millipore, MAB374; α-Tubulin, Santa Cruz sc-5286.

Shen Z., Kang J., Shakya A., Tabaka M., Jarboe E.A., Regev A, & Tantin D. (2017). Enforcement of developmental lineage specificity by transcription factor Oct1. eLife, 6, e20937.

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Immunofluorescence Staining of Epithelial Markers

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Cells were fixed with 4% polyformaldehyde for 30 min at room temperature, permeabilized, and blocked using phosphate-buffered saline solution containing 0.3% Triton X-100 and 3% bovine serum albumin for 1 hour at room temperature. Following overnight incubation with primary antibodies, cells were incubated with secondary antibodies for 1 hour at room temperature. Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole. Primary antibodies used for immunofluorescence were GRHL3 (Sigma-Aldrich, HPA059960; 1:500), KRT8 (Invitrogen, MA514428; 1:500), KRT18 (Invitrogen, MA512104; 1:500), KRT7 (Cell Signaling Technology, 4465S; 1:500), KRT19 (Thermo Fisher Scientific, MS-1902-P; 1:1000), NANOG (GeneTex, GTX100863; 1:500), TP63 (GeneTex, GTX102425; 1:500), ADD2 (Proteintech, 14640-1-AP; 1:500), TUBB4A (Abcam, ab179509; 1:500), DSP (Proteintech, 25318-1-AP; 1:500), and COL3A1 (Abcam, ab184993; 1:500). Secondary antibodies were from the Alexa Fluor series (Cell Signaling Technology; 1:1000).

Huang H., Liu J., Li M., Guo H., Zhu J., Zhu L., Wu S., Mo K., Huang Y., Tan J., Chen C., Wang B., Yu Y., Wang L., Liu Y, & Ouyang H. (2022). Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment. Science Advances, 8(28), eabo5668.

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