T12 microscope

Manufactured by Ametek

The T12 microscope is a precision instrument designed for accurate and reliable microscopic analysis. It features a high-resolution optical system, providing clear and detailed imaging capabilities. The T12 is a versatile tool suitable for a wide range of laboratory applications.

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Lab products found in correlation

Ultrascan 895, by Ametek (1 mentions) Ccd camera, by Ametek (1 mentions) Ultrascan 4000, by Ametek (1 mentions) Orius ccd camera, by Ametek (1 mentions) Ultrascan 4k ccd camera, by Ametek (1 mentions) Dts1070 folded capillary cell, by Malvern Panalytical (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions) Zetasizer nano, by Malvern Panalytical (1 mentions) Ccd image system, by Ametek (1 mentions) Q500 thermal analysis system, by TA Instruments (1 mentions) D8 advance, by Bruker (1 mentions) Equinox 55 ft ir spectrometer, by Bruker (1 mentions)

6 protocols using t12 microscope

Visualizing VWF Tubules by Electron Microscopy

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VWF tubules (3 μL at 0.01 to 0.3 mg/mL) were deposited onto glow discharged (15 seconds at 30 mA voltage) grids (EMS, #CF200-Cu). After 1 minute, the grids were blotted with filter paper, washed twice with 3 μL of 1.5% uranyl formate (UF), incubated with 1.5% UF for 90 seconds, then blotted again. Grids were imaged on a Tecnai T12 microscope equipped with a Gatan UltraScan 895 camera. Particle lengths were measured using Fiji.¹⁵ (link)

Anderson J.R., Li J., Springer T.A, & Brown A. (2022). Structures of VWF tubules before and after concatemerization reveal a mechanism of disulfide bond exchange. Blood, 140(12), 1419-1430.

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Negative Staining for Electron Microscopy

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Negative stained grids were prepared using carbon-coated copper grids that had been glow-discharged for 30 s. The sample was blotted and subsequently stained with two drops of 1% Uranyl acetate before drying and imaging. The grids were imaged using a FEI T12 microscope fitted with a 2 K × 2 K. Gatan CCD camera operating at 120 kV was used to examine the samples.

Berrout J., Kyriakopoulou E., Moparthi L., Hogea A.S., Berrout L., Ivan C., Lorger M., Boyle J., Peers C., Muench S., Gomez J.E., Hu X., Hurst C., Hall T., Umamaheswaran S., Wesley L., Gagea M., Shires M., Manfield I., Knowles M.A., Davies S., Suhling K., Gonzalez Y.T., Carragher N., Macleod K., Abbott N.J., Calin G.A., Gamper N., Zygmunt P.M, & Timsah Z. (2017). TRPA1–FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3p. Nature Communications, 8, 947.

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Negative-Stain Electron Microscopy of Nanorulers

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For negative-stain electron microscopy, agarose gel-purified nanorulers were incubated on freshly glow-discharged carbon-coated 400 mesh copper grids for 1 min. Afterward the sample was blotted off and a 0.75% uranyl formate solution was applied immediately for staining and blotted off without incubation. This staining was repeated four times and followed by a last incubation for which the stain was incubated for 45 s before blotting. Samples were air-dried before imaging. The data were collected at the University of California, San Francisco on a Tecnai T12 microscope operating at 120 kV, using a 4,000 × 4,000 charge-coupled device camera (UltraScan 4000; Gatan).

Niekamp S., Stuurman N., Zhang N, & Vale R.D. (2021). Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility. Proceedings of the National Academy of Sciences of the United States of America, 118(31), e2101391118.

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Quantitative Ultrastructural Analysis of Infected Cells

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Thin sections (90 nm) were used for screening and quantitative analysis of cell cross sections in 2D. Sections were collected on formvar-coated copper slot grids and contrasted with 2% aqueous uranyl acetate and Sato's lead. Imaging was performed on a Philips CM-100 microscope operating at 100 kV or a Tecnai T12 microscope operating at 80 kV, both equipped with a Gatan Orius CCD camera. For quantitative analysis, 25 cross sections of infected cells were imaged. For each infected cell, a series of overlapping images were collected covering the entire cell cross section at a magnification high enough to identify DMVs and virus particles. Images were montaged together using the MosiacJ plugin of ImageJ (Thévenaz and Unser, 2007 (link)), and the montage of each cell was imported into IMOD (Kremer et al., 1996 (link)). The plasma membrane and nucleus were hand traced as single contours, and the locations of DMVs and virus particles were marked using point objects. Cytoplasmic area was determined by subtracting the area inside the nuclear trace from the area inside the plasma membrane trace. DMV density was determined as the number of DMVs counted per μm² of cytoplasmic area. Statistical significance between groups was determined using the Wilcoxen rank sum test.

Mihelc E.M., Baker S.C, & Lanman J.K. (2020). Coronavirus infection induces progressive restructuring of the endoplasmic reticulum involving the formation and degradation of double membrane vesicles. Virology, 556, 9-22.

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Particle Characterization Using Light Scattering

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Static and dynamic light scattering measurements were made using a Malvern Zetasizer and 1 cm x 1 cm plastic cuvettes. Zeta potential measurements were made using a Malvern Zetasizer and a DTS1070 folded capillary cell. During light scattering and zeta potential measurements, the detector was placed at an angle of 173° from the 632.8 nm incident laser. Surface tension measurements were acquired using a Nima Type 611 trough with a PS4 pressure sensor (Coventry, England) with Bolin Scientific paper Wilhelmy plates (Bolin Scientific, Gothenburg Sweden) or a Kruss K10T Tensiometer (Matthews, NC) with a platinum Du Noüy ring or Wilhelmy plate. TEM images were acquired using a Techni T12 Microscope and Gatan 4k Ultrascan CCD camera.

Gahan C.G., Patel S.J., Boursier M.E., Nyffeler K.E., Jennings J., Abbott N.L., Blackwell H.E., Van Lehn R.C, & Lynn D.M. (2020). Bacterial Quorum Sensing Signals Self-Assemble in Aqueous Media to Form Micelles and Vesicles: An Integrated Experimental and Molecular Dynamics Study.. The journal of physical chemistry. B, 124(18), 3616-3628.

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Characterization of Iron Oxide Nanoparticles

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Transmission electron microscopy (TEM) samples were prepared by pipetting a 10 μL drop of as-synthesized NPs at a concentration of 0.125 mg/mL onto a copper TEM grid with lacey carbon film. TEM was conducted on a FEI T12 microscope operated at 120 kV, equipped with a Gatan CCD image system with digital micrograph software program. Powder X-ray diffraction (PXRD) patterns were collected on as-synthesized Fe₃O₄ NPs deposited on a glass substrate using a Bruker D8 Advance using Cu Kα radiation (λ= 1.5418 Å), with the background from the glass substrate subtracted. Transmission Fourier transform infrared (FTIR) spectroscopy measurements were recorded on a Bruker Equinox 55 FT-IR spectrometer in the range of 4000 cm⁻¹ to 400 cm⁻¹ at 2 cm⁻¹ resolution on NPs in a potassium bromide (KBr) pellet, at a sample mass loading of 0.33 wt.%. Thermogravimetric analysis (TGA) was carried out using a TA Instruments Q500 thermal analysis system under a N₂ atmosphere and at a constant heating rate of 10 °C/min from 100 °C to 600 °C. All samples were first heated to 100 °C and held at that temperature for 3 min to remove adsorbed water. Dynamic light scattering (DLS) and zeta-potential (ζ-potential) measurements was carried out using a Malvern Zetasizer Nano and three independent measurements were made for each sample.

Roberts D.S., Chen B., Tiambeng T.N., Wu Z., Ge Y, & Jin S. (2019). Reproducible Large-Scale Synthesis of Surface Silanized Nanoparticles as an Enabling Nanoproteomics Platform: Enrichment of the Human Heart Phosphoproteome. Nano research, 12(6), 1473-1481.

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