A total of 20 μg of protein was submitted to SDS-PAGE 12% in a Mini-Protean electrophoresis module (Bio-Rad). Precision Plus Proteins WesternC Standard (Bio-Rad) was used as marker. The gel was further stained with Coomassie Colloidal G-250 (Sigma-Aldrich).
Precision plus proteins westernc standard
Manufactured by Bio-Rad
Precision Plus Proteins WesternC Standard is a pre-stained protein molecular weight standard used for Western blotting analysis. It provides consistent molecular weight markers for accurate protein size determination.
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Lab products found in correlation
2 protocols using precision plus proteins westernc standard
1
Protein Separation and Visualization
2
Protein Quantification and SDS-PAGE Analysis
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The total protein extracts were quantified by the Bradford method [19 (link)] and 20 μg of protein from each sample was resolved on a polyacrylamide gel under denaturing conditions (SDS-PAGE) at 12%. The marker used was the Precision Plus Proteins™ WesternC™ Standard from Bio-Rad. The gel was stained using the Coomassie Colloidal from Sigma-Aldrich.
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