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Rabbit anti clathrin

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Rabbit anti-clathrin is a primary antibody that binds to the clathrin protein. Clathrin is a structural protein involved in the formation of vesicles during endocytosis. This antibody can be used to detect and study clathrin in various experimental applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Sheep anti n cadherin, by R&D Systems (1 mentions) Mouse anti sars cov 2 spike, by GeneTex (1 mentions) Alexa647 coupled phalloidin, by Thermo Fisher Scientific (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Mouse anti hrs, by Enzo Life Sciences (1 mentions) Rabbit anti eea1, by Cell Signaling Technology (1 mentions) Mouse anti eea1, by BD (1 mentions) Anti chmp4b, by Proteintech (1 mentions)

2 protocols using rabbit anti clathrin

1

Comprehensive Antibody Characterization for Cellular Analysis

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The following antibodies were used: rabbit anti-Vps34 (Immobilon); guinea pig anti-megalin (81 (link)); rabbit anti-clathrin (Abcam, Cambridge UK, ab 21679); rabbit anti-EEA1 and rabbit anti-Rab11 both purchased from Invitrogen, rat anti-Lamp2 (sc-19991, Santa Cruz Biotechnology), mouse-anti NKA (MerckMillipore, Germany); rabbit anti-NBC1 (Alomone labs), rabbit and guinea pig anti-SGLT2 (H. Köpsell, University Würzburg), rabbit anti-S6P (5G10), rabbit anti-phospho-S6P (D57.2.2E), both purchased from Cell Signaling Technologies; sheep anti-NDRG1, sheep anti-phospho-NDRG1 (gift of D. Alessi, University of Dundee, UK); rabbit anti-B0AT1 (82 (link)); rabbit anti-y+LAT1 (83 (link)); 4F2hc (CD98, M-20, Santa Cruz Biotechnology, Heidelberg, Germany) goat anti-Alix (N-20, sc-49267, Santa Cruz Biotechnology, Heidelberg, Germany); goat anti-Vps35 and goat anti-galectin-3 (Bio-Techne, Abingdon, UK); mouse anti-SARS-CoV-2 Nucleoprotein Antibody (ProSci, 35–579); sheep anti-N-Cadherin (R&D Systems, AF6426); rabbit anti-Megalin (Sigma, SAB1305050); mouse anti-SARS-CoV-2 spike (GeneTex, GTX632604); rabbit anti-cleaved caspase-3 (Cell Signaling, 9661). Alexa647-coupled phalloidin (ThermoFischer Scientific, Zug, Switzerland) and DAPI (Thermo Scientific, 62248) were used to stain actin and nuclei, respectively.
Aruffo A., Kanner S.B., Sgroi D., Ledbetter J.A, & Stamenkovic I. (1992). CD22-mediated stimulation of T cells regulates T-cell receptor/CD3-induced signaling.. Proceedings of the National Academy of Sciences of the United States of America, 89(21), 10242-10246.
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2

Immunofluorescence Staining Reagents and Plasmids

Cited 1 time
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Rabbit anti-Hrs was raised using GST–Hrs as an immunogen and was generated by Eurogentec, Southampton, UK. The antibody was affinity-purified against GST–Hrs and adsorbed against GST. Sheep anti-VPS28 was as described previously (Bishop et al., 2002 (link)). Mouse anti-Hrs was from Enzo. Rabbit anti-EEA1 was from Cell Signaling Technology. Mouse anti-EEA1 was from BD Biosciences. Mouse anti-EGFR was Mab 108, purified from supernatants of the hybridoma clone HB-9764 (ATCC; Washington DC), grown in RPMI medium and 10% FCS under 5% CO2, and antibody affinity-purified using Protein-G–Sepharose. Rabbit anti-APPL1 and anti-CHMP4B were from Proteintech Europe (Manchester, UK). Rabbit anti-clathrin was from Abcam (Cambridge, UK). Fluorescent EGF was from Invitrogen (Paisley, Scotland) and secondary antibodies were from Jackson ImmunoResearch (West Grove, PA). Human SNX15 in pEGFP-C1 (Clontech) was a gift from Chris Danson and Pete Cullen, University of Bristol.
Flores-Rodriguez N., Kenwright D.A., Chung P.H., Harrison A.W., Stefani F., Waigh T.A., Allan V.J, & Woodman P.G. (2015). ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome. Journal of Cell Science, 128(4), 755-767.
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