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Suc leu leu 5 al tyr aminomethylcoumarin suc llvy amc

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Suc-Leu-Leu-Val-Tyr-aminomethylcoumarin (Suc-LLVY-AMC) is a fluorogenic substrate used for the detection and measurement of chymotrypsin-like activity in biological samples. It consists of a peptide sequence (Suc-LLVY) coupled to the fluorescent reporter molecule aminomethylcoumarin (AMC).

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Lab products found in correlation

Z leu leu glu amc, by R&D Systems (3 mentions) Anti ha tag, by Bioworld Technology (3 mentions) Anti gapdh, by Bioworld Technology (3 mentions) Bortezomib, by BD (2 mentions) B ap15, by Merck Group (2 mentions) Boc leu arg arg amc, by R&D Systems (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Cisplatin, by Merck Group (2 mentions) Bilirubin, by Merck Group (1 mentions) Biliverdin, by Merck Group (1 mentions) Ha ubvs, by R&D Systems (1 mentions) Anti ubiquitin p4d1, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence ecl reagent, by Santa Cruz Biotechnology (1 mentions) K48 linked tetraubiquitin, by R&D Systems (1 mentions) Mts assay kit celltiter 96 aqueous one solution reagent, by Promega (1 mentions) Proteasome glo chymotrypsin like cell based assay kit, by Promega (1 mentions)

Close spelling variants of this product

Suc-LLVY-AMC

3 protocols using suc leu leu 5 al tyr aminomethylcoumarin suc llvy amc

1

Ubiquitin-Proteasome System Assay

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PtPT was synthesized in our lab. Other agents are bortezomib (BD Biosciences, San Jose, CA); NEM, Penicillin, Streptomycin, Cisplatin (Sigma–Aldrich Inc., St. Louis, MO); b-AP15, Suc-Leu-Leu-V al-Tyr-aminomethylcoumarin (Suc-LLVY-AMC), Z-Leu-Leu-Glu-AMC (Z-LLE-AMC), Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC), 19S, 20S and 26S human proteasomes, HA-Ubiquitin-Vinyl Sulfone (HA-Ub-VS), K48-linked tetra-ubiquitin, ubiquitin-AMC (U550) (BostonBiochem, Cambridge, MA). Antibodies used in this study were purchased from following sources: anti-ubiquitin (P4D1), anti-p27 (F-8), anti-GFP (B-2) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-p21 Waf1/Cip1 (DCS60), anti-caspase3 (8G10), anti-caspase8 (1C12), anti-caspase9 (C9), anti-PARP, anti-K48-linkage specific polyubiquitin (D9D5), anti-phospho-histone H2AX (Ser139) (20E3), anti-phospho-ATM (Ser1981) (D6H9), anti-phospho-Chk1 (Ser345) (133D3), anti-phospho-Chk2 (Thr68) (C13C1) (Cell Signaling Technology, Beverly, MA, USA); anti-GAPDH, anti-HA-tag (Bioworld Technology, Inc., St. Louis Park, MN, USA). Propidium iodide (PI) and Annexin V-FITC/PI apoptosis detection Kit were purchased from Keygen Company (Nanjing, China). 4,6-Diamidino-2-phenylindole (DAPI) was from Invitrogen (Guangzhou, China). Enhanced chemiluminescence (ECL) reagents were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
Zhao C., Chen X., Zang D., Lan X., Liao S., Yang C., Zhang P., Wu J., Li X., Liu N., Liao Y., Huang H., Shi X., Jiang L., Liu X., He Z., Wang X, & Liu J. (2016). Platinum-containing compound platinum pyrithione is stronger and safer than cisplatin in cancer therapy. Biochemical pharmacology, 116, 22-38.
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2

Proteasome Inhibition and Ubiquitination Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bilirubin and biliverdin were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Other agents used include NEM (Sigma-Aldrich Inc.), the Proteasome-Glo Chymotrypsin-like Cell-Based Assay kit (Promega Bioscience, Madison, WI, USA), Suc-Leu-Leu-Val-Tyr-aminomethylcoumarin (Suc-LLVY-AMC), Boc-Leu-Arg-Arg- aminomethylcoumarin (Boc-LRR-AMC), Z-Leu-Leu-Glu-AMC, 20S and 26S human proteasome preparations, HA-Ub-VS, K48-linked tetraubiquitin, Ubiquitin-AMC (U550) (Boston Biochem, Cambridge, MA, USA), JC-1 (Beyotime, Shanghai, China) and MTS assay kit (CellTiter 96 Aqueous One Solution reagent) (Promega Corporation, Madison, WI, USA). Antibodies used in this study and their sources are: anti-ubiquitin (P4D1) (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-K48-linkage specific polyubiquitin (D9D5), anti-Bax (D3R2M) (Cell Signaling Technology, Beverly, MA, USA), anti-GAPDH, anti-HA-tag (Bioworld Technology, Inc., Louis Park, MN, USA) and anti-Tau-1, (Invitrogen, Carlsbad, CA, USA). Enhanced chemiluminescence (ECL) reagents were purchased from Santa Cruz Biotechnology Inc.
Huang H., Guo M., Liu N., Zhao C., Chen H., Wang X., Liao S., Zhou P., Liao Y., Chen X., Lan X., Chen J., Xu D., Li X., Shi X., Yu L., Nie Y., Wang X., Zhang C.E, & Liu J. (2017). Bilirubin neurotoxicity is associated with proteasome inhibition. Cell Death & Disease, 8(6), e2877-.
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3

Ubiquitin-Proteasome System Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
PtPT was synthesized in our lab. Other agents are bortezomib (BD Biosciences, San Jose, CA); NEM, Penicillin, Streptomycin, Cisplatin (Sigma–Aldrich Inc., St. Louis, MO); b-AP15, Suc-Leu-Leu-V al-Tyr-aminomethylcoumarin (Suc-LLVY-AMC), Z-Leu-Leu-Glu-AMC (Z-LLE-AMC), Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC), 19S, 20S and 26S human proteasomes, HA-Ubiquitin-Vinyl Sulfone (HA-Ub-VS), K48-linked tetra-ubiquitin, ubiquitin-AMC (U550) (BostonBiochem, Cambridge, MA). Antibodies used in this study were purchased from following sources: anti-ubiquitin (P4D1), anti-p27 (F-8), anti-GFP (B-2) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-p21 Waf1/Cip1 (DCS60), anti-caspase3 (8G10), anti-caspase8 (1C12), anti-caspase9 (C9), anti-PARP, anti-K48-linkage specific polyubiquitin (D9D5), anti-phospho-histone H2AX (Ser139) (20E3), anti-phospho-ATM (Ser1981) (D6H9), anti-phospho-Chk1 (Ser345) (133D3), anti-phospho-Chk2 (Thr68) (C13C1) (Cell Signaling Technology, Beverly, MA, USA); anti-GAPDH, anti-HA-tag (Bioworld Technology, Inc., St. Louis Park, MN, USA). Propidium iodide (PI) and Annexin V-FITC/PI apoptosis detection Kit were purchased from Keygen Company (Nanjing, China). 4,6-Diamidino-2-phenylindole (DAPI) was from Invitrogen (Guangzhou, China). Enhanced chemiluminescence (ECL) reagents were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).
Zhao C., Chen X., Zang D., Lan X., Liao S., Yang C., Zhang P., Wu J., Li X., Liu N., Liao Y., Huang H., Shi X., Jiang L., Liu X., He Z., Wang X, & Liu J. (2016). Platinum-containing compound platinum pyrithione is stronger and safer than cisplatin in cancer therapy. Biochemical pharmacology, 116, 22-38.
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