Polycaprolactone (PCL) pellets (MW ~80,000) were purchased from Perstorp UK Limited (Warrington, UK), while glacial acetic acid (GAC) (purity > 99.5%) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Alginate was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Levofloxacin (purity > 98%) was obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Universal red ink was purchased from Shenzhen Caiju Electronic Technology Co., Ltd. (Shenzhen, China). All reagents were used in the experiments without further processing.
Levofloxacin
Manufactured by Maclin Biochemical Technology
Sourced in China
Levofloxacin is a broad-spectrum antibiotic medication that belongs to the fluoroquinolone class of antibacterial agents. It is used to treat various types of bacterial infections. Levofloxacin functions by inhibiting bacterial DNA gyrase and topoisomerase IV, which are essential enzymes involved in bacterial DNA replication and transcription.
Automatically generated - may contain errors
3 protocols using levofloxacin
1
Polycaprolactone-based Alginate Hydrogel for Levofloxacin Delivery
2
Synthesis and Characterization of CMCS-PGA Nanoparticles
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Carboxymethyl chitosan (CMCS, 1000 kDa) was synthesized according to a method reported in the literature [40 (link)]. The substitution degree of the carboxymethyl was determined as 0.75 by elemental analysis. Poly(γ-glutamic acid) (PGA, >92.0%, 1000 kDa) was purchased from Nanjing Saitaisi Biotechnology Co., Ltd. (Nanjing, China); Aluminum sulfate was purchased from Tianjin Fu Chen Chemical Reagent Factory (Tianjing, China); Levofloxacin (LFX, 98%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).
3
Bioluminescent Bacterial Strain Characterization
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The bioluminescent strain PAO1 carrying luxCDABE gene operon was purchased from Caliper Life Sciences, USA. The carbapenem-resistant and bioluminescent strain PAO1 (CRPAO1) was cultured as previously described.14 (link) PA ATCC 27853 was used as a quality control strain.
Amikacin, meropenem, levofloxacin (Shanghai Macklin Biochemical Co., Ltd., China), and colistin sulfate (Sigma, China) were used in our present study. Mueller-Hinton Agar and adjusted Mueller-Hinton Broth (MHB) were used to culture bacteria that were purchased from Becton, Dickinson and Company. Isoflurane (Shenzhen RWD Life Science Company, China) and pentobarbital (Shanghai Rongchuang Biotechnology Company, China) were used to anesthetize mice.
Amikacin, meropenem, levofloxacin (Shanghai Macklin Biochemical Co., Ltd., China), and colistin sulfate (Sigma, China) were used in our present study. Mueller-Hinton Agar and adjusted Mueller-Hinton Broth (MHB) were used to culture bacteria that were purchased from Becton, Dickinson and Company. Isoflurane (Shenzhen RWD Life Science Company, China) and pentobarbital (Shanghai Rongchuang Biotechnology Company, China) were used to anesthetize mice.
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