Nod rag1null il2rgnull nrg

NOD-Rag1^null IL2rg^null (NRG; Jackson Laboratory) and Nt5e^-/- C57BL/6 J mice (obtained from Dr. Linda Thompson; OMRF, Oklahoma City, OK, USA) were bred and housed at the CRCHUM which holds a certificate of Good Animal Practice from the Canadian Council on Animal Care (CCAC). C57BL/6 J mice were purchased from Jackson Laboratory and housed at the CRCHUM. All the experimental procedures were authorized, and all animals were handled according to an approved Institutional Animal Care and Use Committee (IACUC) protocol (#C20010JSs) of the Centre de Recherche du Centre Hospitalier de l'Université de Montréal. For in vivo tumor growth, 8–12 weeks-old healthy female NRG mice were injected with 2x10⁶ MDA-MB-231 cells sub-cutaneously and tumor growth was monitored thrice weekly. Tumor volume was measured by caliper in two dimensions, and volumes were estimated using the equation V = (large diameter ×small diameter²)×0.5. The total number of mice analyzed for each experiment is detailed in figure legends. C57BL/6 J and Nt5e^-/- C57BL/6 J mice were used for mouse embryoblastic fibroblast (MEF) derivation (see cell lines generation and culture section).

NOD-Rag1^null IL2rg^null (NRG; Jackson Laboratory) and Nt5e^-/- C57BL/6 J mice (obtained from Dr. Linda Thompson; OMRF, Oklahoma City, OK, USA) were bred and housed at the CRCHUM which holds a certificate of Good Animal Practice from the Canadian Council on Animal Care (CCAC). C57BL/6 J mice were purchased from Jackson Laboratory and housed at the CRCHUM. All the experimental procedures were authorized, and all animals were handled according to an approved Institutional Animal Care and Use Committee (IACUC) protocol (#C20010JSs) of the Centre de Recherche du Centre Hospitalier de l'Université de Montréal. For in vivo tumor growth, 8–12 weeks-old healthy female NRG mice were injected with 2x10⁶ MDA-MB-231 cells sub-cutaneously and tumor growth was monitored thrice weekly. Tumor volume was measured by caliper in two dimensions, and volumes were estimated using the equation V = (large diameter ×small diameter²)×0.5. The total number of mice analyzed for each experiment is detailed in figure legends. C57BL/6 J and Nt5e^-/- C57BL/6 J mice were used for mouse embryoblastic fibroblast (MEF) derivation (see cell lines generation and culture section).