Plasma concentrations of TNF were measured using V-PLEX Human Proinflammatory Panel 1 and plasma concentrations of TNFR1 and TNFR2 were measured using Human TNFR-I ultra-sensitive kit and Human TNFR-II ultra-sensitive kit. Interleukin (IL)-6 and vascular endothelial growth factor (VEGF-A) were measured using the V-Plex Human IL-6 kit and V-Plex Human VEGF kit (all from Mesoscale Discovery, Rockville, MD, USA). Serum concentrations of CCL2 were measured using a V-PLEX human MCP-1 kit (Mesoscale Discovery). Samples were diluted in Diluent-41 and analyzed in duplicate according to the manufacturer’s instructions. Analysis was performed on a SECTOR Imager 6000 plate reader and MSD Discovery Workbench software was used for analysis (Mesoscale Discovery). Sample replicates with coefficient of variation (CV) values >25% in individual analyses were excluded. None of the measurements were below the lower level of detection.
V plex human vegf kit
Manufactured by Mesoscale
Sourced in United States
The V-PLEX Human VEGF Kit is a laboratory equipment product designed to quantify the levels of Vascular Endothelial Growth Factor (VEGF) in human samples. It utilizes an electrochemiluminescence-based assay to measure VEGF concentrations with high sensitivity and specificity.
Automatically generated - may contain errors
3 protocols using v plex human vegf kit
1
Quantification of Inflammatory Markers
2
VEGF Quantification in Infant Plasma
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In infants who received initial ranibizumab treatment with an even subject identification number plus infants who underwent initial laser therapy, the blood samples were collected at the following time points: day 1 (within 24 hours before the first administration), day 15 (days 7–21), and day 29 (days 22–28).
The concentration of free VEGF (i.e., VEGF that is not bound to ranibizumab) was determined in human K3-ethylenediaminetetraacetic acid (K3-EDTA) plasma samples by an electrochemiluminescence sandwich immunoassay (ECLA) using the V-PLEX Human VEGF Kit (MesoScale Discovery, Rockville, MD, USA). Diluted samples were loaded to wells coated with monoclonal anti-VEGF capture antibodies. Sulfo-tag-labeled anti-human VEGF detection antibodies were added, and the resulting ECLA reaction detected using an MSD reader (MesoScale Discovery). The assay quantification range was from 5.99 pg/mL (lower limit of quantitation, LLOQ) to 253 pg/mL in 100% K3-EDTA plasma.
The concentration of free VEGF (i.e., VEGF that is not bound to ranibizumab) was determined in human K3-ethylenediaminetetraacetic acid (K3-EDTA) plasma samples by an electrochemiluminescence sandwich immunoassay (ECLA) using the V-PLEX Human VEGF Kit (MesoScale Discovery, Rockville, MD, USA). Diluted samples were loaded to wells coated with monoclonal anti-VEGF capture antibodies. Sulfo-tag-labeled anti-human VEGF detection antibodies were added, and the resulting ECLA reaction detected using an MSD reader (MesoScale Discovery). The assay quantification range was from 5.99 pg/mL (lower limit of quantitation, LLOQ) to 253 pg/mL in 100% K3-EDTA plasma.
3
Measuring Endothelial Activation Markers
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Soluble endothelial adhesion molecules, including vascular cell adhesion molecule 1 (VCAM1), E-selectin, and vascular endothelial growth factor A (VEGFA) were measured to assess endothelial activation and function (Hwang et al., 1997; Kliche and Waltenberger, 2001; Meigs et al., 2004; Ponthieux et al., 2004) . Samples were analysed using commercially available kits from Mesoscale (V-PLEX Plus Human VCAM-1 Kit, Human E-Selectin Kit, and V-PLEX Human VEGF Kit) according to the manufacturer's instructions. Samples were run in duplex and diluted 2-fold in Diluent 41 prior to measurement. Data were analysed using MSD Discovery Workbench software. The lower limit of detection was a calculated concentration based on a signal 2.5 standard deviations (SD) above the blank (zero) calibrator.
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