Growth medium for bacterial cultures (LB broth, LB agar, EMB agar, SS agar, TCBS agar, Deca strength phage broth, Mueller Hinton Agar) and Bovine Serum Albumin (BSA) were purchased from Himedia Laboratories. DMEM medium, agarose resins (Gelatin–agarose, Heparin agarose and Sepharose 4B), Resazurin and Triton X 100 were procured from Sigma Aldrich. Fetal Bovine Serum (FBS), Penicillin-Streptomycin mixture (PenStrep from Gibco), Amphotericin B and SYBR green master mix 2X DyNAmo Color Flash were obtained from Gibco, Thermo Fisher Scientific. All other salts and other reagents were procured from Sigma Aldrich.
Ss agar
Manufactured by HiMedia
Sourced in India
SS agar is a selective and differential culture medium used for the isolation and identification of Salmonella and Shigella species. It contains brilliant green, bile salts, and thiosulfate, which inhibit the growth of most gram-positive bacteria and coliforms, allowing for the selective growth of Salmonella and Shigella. The medium also allows for the differentiation of these organisms based on their ability to ferment lactose and produce hydrogen sulfide.
Automatically generated - may contain errors
Lab products found in correlation
Emb agar, by HiMedia (2 mentions)
Agarose resins, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
Heparin agarose, by Merck Group (1 mentions)
Lb agar, by HiMedia (1 mentions)
Sepharose 4b, by Merck Group (1 mentions)
Gelatin agarose, by Merck Group (1 mentions)
Tcbs agar, by HiMedia (1 mentions)
Dmem medium, by Merck Group (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Lb broth, by HiMedia (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Mueller hinton agar (mha), by HiMedia (1 mentions)
Bovine serum albumin (bsa), by HiMedia (1 mentions)
Mrs agar, by HiMedia (1 mentions)
Nutrient agar, by HiMedia (1 mentions)
3 protocols using ss agar
1
Bacterial Culture Growth Media Preparation
2
Salmonella Disinfection using Bacteriophage
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Multi-drug resistant clinical strain of Salmonella enterica was used in the study. The strain was cultured in the Luria Bertani (LB) broth at 37 °C for 16–18 h in a shaking incubator at 250 rpm. The culture was subcultured in Salmonella-Shigella Agar (SS Agar, HiMedia, India) and stored at 4 °C throughout the experiment. Bacteriophage φPh_Se01 previously reported for its ability to reduce biogenic H2S produced by S. enterica was employed for the disinfection experiments [25 (link)]. The phage was stored and maintained at 4 °C for immediate use and at −80 °C for long term storage.
3
Enumeration of Gut Microbiota in Poultry
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The pre-caecal contents of the intestine were collected aseptically from the birds after slaughter on 42 nd day of the trial. The pre-caecal contents were stored at 4 o C and bacteriological enumeration was performed on the same day. Serial dilution of 1 g of the pre-caecal content was done (10-fold) with sterile phosphate buffer saline (PBS). Each dilution (10 L) was poured on Nutrient agar (HiMedia, India), MRS agar (HiMedia, India), SS agar (HiMedia, Mumbai, India) and EMB agar (HiMedia, Mumbai, India), for finding the count of total bacteria, Lactobacillus spp., Salmonella spp. and E.coli respectively. The plates were incubated aerobically at 37°C for 24 h. The colonies of bacteria were enumerated in a colony counter (hand held digital colony counter, LA663, Himedia Lab. Pvt. Ltd., Mumbai, India) and the numbers were expressed as Log 10 colony forming units (CFU) per gram of sample.
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