Selected calcium-imaged cells were dye-filled (Alexa Fluor 568 hydrazide; Invitrogen) using fine glass pipettes (~50 MΩ resistance) guided by two-photon imaging. A current pulse (-20 nA; 100 ms) triggered cell penetration. Dye was iontophoretically injected (20 - 60 biphasic current pulses; ‐2000 pA for 500 ms and +500 pA for 400 ms) until dendrites were well filled. After all calcium imaging was completed, filled cells were documented in Z-stacks acquired in confocal (single-photon) mode.
Digidata 1550 digitizer
The Digidata 1550 is a high-performance digitizer designed for acquiring and recording electrophysiological data. It features simultaneous acquisition of up to 32 analog channels and 16 digital channels, with a maximum sampling rate of 500 kHz per channel.
Lab products found in correlation
14 protocols using digidata 1550 digitizer
Two-Photon Imaging and Patch-Clamp Electrophysiology
Two-Electrode Voltage Clamp Analysis of mRNA-Injected Oocytes
Electrophysiological Characterization of Locus Coeruleus
Patch-clamp Analysis of Induced Neuronal Cells
Electrophysiological Characterization of iPSC-Derived Neurons
Whole-cell Patch-clamp Recordings of ICX Neurons
Patch-clamp Analysis of Induced Neuronal Cells
Electrophysiological Characterization of Locus Coeruleus
Two-Photon Imaging and Patch-Clamp Electrophysiology
Selected calcium-imaged cells were dye-filled (Alexa Fluor 568 hydrazide; Invitrogen) using fine glass pipettes (~50 MΩ resistance) guided by two-photon imaging. A current pulse (-20 nA; 100 ms) triggered cell penetration. Dye was iontophoretically injected (20 - 60 biphasic current pulses; ‐2000 pA for 500 ms and +500 pA for 400 ms) until dendrites were well filled. After all calcium imaging was completed, filled cells were documented in Z-stacks acquired in confocal (single-photon) mode.
Somatosensory Cortex Pyramidal Neuron Electrophysiology
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