Doxycycline

Manufactured by HiMedia

Sourced in India

Doxycycline is a tetracycline-class antibiotic used in laboratory settings. It inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. Doxycycline is commonly used in microbiological research and testing applications.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using doxycycline

Enzymatic Assay for Cysteine Sulfoxide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reduced
form of nicotinamide adenine dinucleotide
(NADH), lactate dehydrogenase (LDH) from rabbit muscle, and (±)-L-alliin
were purchased from Sigma-Aldrich; pyridoxal 5′-phosphate (PLP)
and d,l-dithiothreitol (DTT) were from Serva; kanamycin
is a domestic product (OAO Biokhimik); DEAE-sepharose was from Amersham.
2-Nitro-5-thiobenzoate (NTB) was prepared according to ref (31 (link)). S-Methyl-l-cysteine sulfoxide (methiin) was synthesized according to
Morozova et al.^{22 (link)} PEG–poly(α,β-aspartic
acid)₇₀ (PEG–P(Asp)₇₀) and poly-(l-lysine)₇₀ (PLL₇₀) were synthesized according
to Koide et al.^{32 (link)} Luria–Bertani
broth (LB), Mueller–Hinton broth, Mueller–Hinton agar,
and antibiotic-impregnated discs: amikacin, amoxycillin/clavulanic
acid, ampicillin, azithromycin, aztreonam, cefepime, ceftazidime,
ceftriaxone, cephotaxime, cephoxitin, chloramphenicol, cipr ofloxacin,
colistin, doxycycline, erythromycin, gentamicin, imipenem, lev ofloxacin,
lincomycin, norfloxacin, ofloxacin, oxacillin, rifampicin, spiramycin,
tobramycin, and vancomycin were from HiMedia Laboratories Pvt. Limited
(India).

Morozova E., Kulikova V., Koval V., Anufrieva N., Chernukha M., Avetisyan L., Lebedeva L., Medvedeva O., Burmistrov E., Shaginyan I., Revtovich S, & Demidkina T. (2020). Encapsulated Methionine γ-Lyase: Application in Enzyme Prodrug Therapy of Pseudomonas aeruginosa Infection. ACS Omega, 5(14), 7782-7786.

+ Open protocol

+ Expand

Antibiotic Susceptibility of Brucella Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols

The brucells isolates were tested for their susceptibility to 11 antibiotics (Rifampicin; Ciprofloxacin; Ampicillin; Erythromycin; Novobiocin; Kanamycin; Gentamicin; Streptomycin; Tetracycline; Doxycycline and Carbenicillin), obtained from Himedia Laboratories. Testing was performed on Mueller-Hinton Agar plates using the Kirby-Bauer disk diffusion technique (Bauer & Kirby, 1966 (link)). The antibiotic resistance of each brucella isolate was determined based on the breakpoints of the inhibition zone diameters for individual antibiotic agents and as recommended by the disk manufacturer.

Al-Afifi A.H., Alewy Almashhadany D., Al-Azazi A.S., Khalaf A.M., Naji Ahmed Odhah M, & Al-Gabri N.A. (2022). Prevalence of Brucella spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen. Italian Journal of Food Safety, 11(4), 10370.

+ Open protocol

+ Expand

Antibiotic Susceptibility Testing of Isolated Organisms

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibiotic susceptibility tests of the isolated organisms were done using Mueller Hinton Agar (MHA) by the standard disk diffusion technique of the Kirby-Bauer method as recommended by CLSI [21 ]. The following antibiotics with specified concentrations were used: ampicillin (10 μg), ampicillin-sulbactam (10/10 μg), amoxycillin-clavulanic acid (20/10 μg), cefixime (5 μg), ceftazidime (30 μg), ceftriaxone (30 μg), cefepime (30 μg), cefoxitin (30 μg), piperacillin (100 μg), piperacillin-tazobactam (100/10 μg), cotrimoxazole (1.25/23.75 μg), gentamicin (10 μg), amikacin (30 μg), imipenem (10 μg), meropenem (10 μg), ciprofloxacin (5 μg), levofloxacin (5 μg), colistin sulfate (10 μg), erythromycin (15 μg), clindamycin (2 μg), vancomycin (30 μg), teicoplanin (30 μg), doxycycline (30 μg), and chloramphenicol (30 μg) from HiMedia Laboratories, India.

Baidya S., Sharma S., Mishra S.K., Kattel H.P., Parajuli K, & Sherchand J.B. (2021). Biofilm Formation by Pathogens Causing Ventilator-Associated Pneumonia at Intensive Care Units in a Tertiary Care Hospital: An Armor for Refuge. BioMed Research International, 2021, 8817700.

+ Open protocol

+ Expand

Antimicrobial Resistance Profiling of Clinical Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antimicrobial susceptibility tests of the clinical isolates against different antimicrobials were performed in Müller–Hinton agar (MHA) using the standard disk diffusion technique (modified Kirby–Bauer method) and interpreted as per Clinical and Laboratory Standards Institute guidelines.16 The following antimicrobial agents were tested: ampicillin (10 μg), cefoxitin (30 μg), ciprofloxacin (5 μg), chloramphenicol (30 μg), clindamycin (2 μg), cotrimoxazole (25 μg), doxycycline (30 μg), erythromycin (15 μg), gentamicin (10 μg), minocycline (30 μg), rifampicin (5 μg), teicoplanin (30 μg), tetracycline (30 μg) and vancomycin (30 μg) (HiMedia Laboratories, Mumbai, Maharashtra, India). S. aureus ATCC 25923 was used as the control organism.
Isolates were considered multidrug resistant (MDR) based on the guidelines recommended by the joint initiative of the European Centre for Disease Prevention and Control (ECDC) and the Centers for Disease Control and Prevention (CDC).17 (link) According to those guidelines, the isolates showing non-susceptibility to at least one agent in three or more antimicrobial categories were identified as MDR.
S. aureus isolates showing positive D zone test were considered as resistant to clindamycin.

Neopane P., Nepal H.P., Shrestha R., Uehara O, & Abiko Y. (2018). In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance. International Journal of General Medicine, 11, 25-32.

+ Open protocol

+ Expand

Antibiotic Susceptibility Profiling of Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibiotic susceptibility profiles of bacteria were tested by disc diffusion method (Baurer et al., 1966 (link)) against 14 commercially available antibiotics such as Ampicillin, Gentamycin, Azithromycin, Sulphatrimethoprim, Vancomycin, Ciprofloxacin, Cephalexin, Doxycycline, Streptomycin, Oxacillin, Erythromycin, Tetracycline, Chloramphenicol and Nalidixic acid (Hi Media, India). Antimicrobial testing results were recorded as susceptible, intermediate and resistant according to zone diameter interpretive standards provided by CLSI (2010 ).

Ali E., Islam M.S., Hossen M.I., Khatun M.M, & Islam M.A. (2021). Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry. Veterinary Medicine and Science, 7(5), 1921-1927.

+ Open protocol

+ Expand

Phytochemical Analysis and Antioxidant Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murashige and Skoog (MS) medium, kinetin, naphthalene acetic acid, pectin, MnCl₂, methanol, toluene, chloroform, ethanol, diethyl ether, benzene, streptomycin, cipr ofloxacin, doxycycline, ampicillin, ofloxacin, dimethyl sulfoxide, DPPH (1,1-diphenyl-2-picrylhydrazyl), sodium acetate, glacial acetic acid, hydrochloric acid, TPTZ (2,4,6-tri[2-pyridyl]-s-triazine), ferrous sulphate, ferric chloride, nitro blue tetrazolium, ethylenediaminetetraacetic acid (EDTA), riboflavin, hydrogen peroxide, methionine, dipotassium phosphate, monopotassium phosphate, and thymol (THY) were purchased from HIMEDIA; thymoquinone (TQ) was purchased from Sigma Aldrich.

Chaudhry H., Fatima N, & Ahmad I.Z. (2015). Evaluation of Antioxidant and Antibacterial Potentials of Nigella sativa L. Suspension Cultures under Elicitation. BioMed Research International, 2015, 708691.

+ Open protocol

+ Expand

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antimicrobial susceptibility testing was performed using the standard Kirby-Bauer disk diffusion method [41 ]. For fastidious organisms, Muller-Hinton agar with 5% sheep blood was used and incubated in 5% CO₂; GC agar and Haemophilus Test Medium were used for Neisseria gonorrhoeae and Haemophilus influenzae, respectively [42 ]. The following antibiotics were used: trimethoprim-sulfamethoxazole (SXT; 1.25/23.75 μg), erythromycin (15 μg), clarithromycin (15 μg), chloramphenicol (30 μg), clindamycin (2 μg), tetracycline (30 μg), doxycycline (30 μg), amikacin (30 μg), gentamicin (10 μg), ciprofloxacin (5 μg), ceftriaxone (30 μg) and cefoxitin (30 μg) (HIMEDIA). The zones of inhibition of the antimicrobial agents were measured using calipers; bacteria were described as susceptible or resistant based on CLSI guideline [42 ]. Multidrug resistance was defined as non-susceptible to ≥1 agent in ≥3 antimicrobial categories according to the definition of Magiorakos and colleagues [43 (link)].

Teweldemedhin M., Saravanan M., Gebreyesus A, & Gebreegziabiher D. (2017). Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates. BMC Infectious Diseases, 17, 207.

+ Open protocol

+ Expand

Antibiotic Susceptibility of A. baumannii

Check if the same lab product or an alternative is used in the 5 most similar protocols

The susceptibility of A. baumannii isolates against different antibiotics was determined by the modified Kirby–Bauer disk diffusion method on Mueller-Hinton agar and interpreted following standard procedures recommended by the Clinical and Laboratory Standards Institute (CLSI), Wayne, USA.11 The antibiotic sensitivity profile of all the isolates of A. baumannii were determined by testing against ampicillin-sulbactam (10/10 μg), ceftazidime (30 μg), gentamicin (10 μg), ciprofloxacin (5 μg), levofloxacin (5 μg), meropenem (10 μg), and imipenem (10 μg). The isolates that were resistant to at least one antimicrobial from three different groups of above-mentioned antibiotics (ie, MDR isolates) were also tested against piperacillin (100 μg), piperacillin-tazobactam (100/10 μg), cefotaxime (30 μg), cefepime (30 μg), cotrimoxazole (25 μg), amikacin (30 μg), doxycycline (30 μg), polymyxin B (300 units), and colistin sulfate (10 μg) from HiMedia Laboratories, India.

Yadav S.K., Bhujel R., Hamal P., Mishra S.K., Sharma S, & Sherchand J.B. (2020). Burden of Multidrug-Resistant Acinetobacter baumannii Infection in Hospitalized Patients in a Tertiary Care Hospital of Nepal. Infection and Drug Resistance, 13, 725-732.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!