Serum samples were analyzed in duplicates according to the manufacturers’ instructions using a MSD Mouse Proinflammatory panel 1 V-Plex kit (MULTI-ARRAY®, Meso Scale Discovery, Gaithersburg, MD) and a Bio-Plex Pro Mouse Diabetic Assay (MAGPIX, Bio-Rad, Copenhagen, Denmark). Serum cytokines, chemokines and hormones analyzed were IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, KC/GRO, and TNF-α using MSD and glucagon, insulin, leptin, monocyte chemotactic protein-1 (MCP-1), and regulated upon activation, normal T cell expressed and secreted (RANTES) using Bio-Plex (Bio-Rad).
Msd mouse proinflammatory panel 1 5 plex kit
Manufactured by Mesoscale
Sourced in United States
The MSD Mouse Proinflammatory panel 1 V-Plex kit is a multiplex assay designed to measure the levels of specific inflammatory biomarkers in mouse samples. The kit utilizes Mesoscale's proprietary electrochemiluminescence technology to quantify the concentrations of these analytes in a single sample.
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Lab products found in correlation
4 protocols using msd mouse proinflammatory panel 1 5 plex kit
1
Multiplex Serum Cytokine Analysis
2
Comprehensive Metabolic and Inflammatory Profiling
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Serum cholesterol, low-density lipoprotein cholesterol and very low-density lipoprotein (LDL/VLDL), glucose, triacylglycerol (TAG), total protein concentrations, uric acid, non-esterified fatty acids (NEFA; D07940, Dialab, Austria) and total antioxidant status (TAS; NX2332 Randox Total Antioxidant Status, Randox Laboratories Ltd., UK) were analysed using conventional enzymatic kits and a MaxMat PLII bioanalyser (MaxMat PL, Montpellier, France). Unless otherwise stated all kits were from MaxMat PL (Montpellier, France). Serum oxidized LDL (Ox-LDL) was quantified in duplicates according to the manufacturers’ instruction with a murine ELISA-kit (E90527Mu, USCN Life Science Inc., Texas, US). Serum samples were analysed in duplicates for the following cytokines: interferon gamma (IFNγ), interleukin 10 (IL-10), interleukin 1 beta (IL-1β), interleukin 2 (IL-2), interleukin 5 (IL-5), interleukin 6 (IL-6), keratinocyte chemoattractant growth-regulated oncogene (KC-GRO), and tumour necrosis factor alpha (TNF-α). These analyses were performed according to the manufacturers’ instructions using a MSD Mouse Proinflammatory panel 1 V-Plex kit (MULTI-ARRAY®, Meso Scale Discovery, Gaithersburg, MD).
3
Profiling Peripheral Inflammation in Mice
4
Peripheral Inflammation Biomarkers Profiling
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Plasma samples collected from three time points: 1) two hours after the first chronic injection (acute), 2) two hours after the final chronic injection (chronic), and 3) from trunk blood at the time of tissue collection (terminal; seven to eight days after a trigger LPS injection used for cognitive testing) to assess markers of peripheral inflammation. Circulating concentrations of IFN-γ, IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, KC/GRO, and TNF-α were assessed using the MSD V-Plex Proinflammatory Panel 1 Mouse Kit (Meso Scale Diagnostics, Rockville, MD, USA; Cat. #: K15048D-2) and read using the MESO QuickPlex SQ 120 (Meso Scale Diagnostics). A standard curve was completed to determine the required dilutions for analysis.
Based on results from the standard curve, samples from saline treated mice were diluted 1:8 for both the acute and chronic time points. Samples from LPS treated mice were diluted 1:20 and 1:30 for acute and chronic time-points respectively. All terminal time points regardless of treatment history were diluted 1:2. MSD workflow was completed according to manufacturer instructions.
Based on results from the standard curve, samples from saline treated mice were diluted 1:8 for both the acute and chronic time points. Samples from LPS treated mice were diluted 1:20 and 1:30 for acute and chronic time-points respectively. All terminal time points regardless of treatment history were diluted 1:2. MSD workflow was completed according to manufacturer instructions.
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