Difco agar

Manufactured by BD

Sourced in United States

Difco agar is a solidifying agent used in the preparation of culture media for the growth and isolation of microorganisms. It is a purified agar derived from red algae, providing a nutrient-rich, gelling substrate for the cultivation of a wide range of microbial species.

Automatically generated - may contain errors

Lab products found in correlation

Bacto yeast extract, by BD (3 mentions) Sucrose for cell growth, by MP Biomedicals (2 mentions) Peptone, by Thermo Fisher Scientific (2 mentions) Copper grid, by Merck Group (1 mentions) Dextrose, by Merck Group (1 mentions) Bacto peptone, by BD (1 mentions) Adenine, by Merck Group (1 mentions) Top agar, by BD (1 mentions) Benzaldehyde, by Merck Group (1 mentions) Bacillus cereus, by RIKEN BioResource Center (1 mentions) Bond elut c18, by Agilent Technologies (1 mentions) Aspergillus niger, by American Type Culture Collection (1 mentions) Biotek synergy h1 microplate reader, by Agilent Technologies (1 mentions) Bacto malt extract, by BD (1 mentions) Fluconazole, by Merck Group (1 mentions) Ham s f12, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions)

Spelling variants of this product

Difco Bacto agar (11 citations) Difco tryptic soy agar (10 citations) Difco Middlebrook 7H10 Agar (8 citations) Difco marine agar 2216 (5 citations) DifcoTM brain heart infusion agar plates (4 citations) Difco plate count agar (4 citations) Difco Middlebrook 7H11 Agar (3 citations) Difco™ Technical Agar (2 citations) Difco oatmeal agar (2 citations) Difco nutrient agar (NA; (2 citations)

18 protocols using difco agar

Pseudomonas aeruginosa Cultivation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 13

Pseudomonas aeruginosa PA01 (ATCC, BAA-47) was used as the model bacterium in this study. Single colony on agar plates, freshly cultured from frozen glycerol stocks was sub-cultured on Nutrient Broth (NB) (Difco NB-BD diagnostics) agar plates (NB, 8 g L⁻¹supplemented with 14 g L⁻¹agar—Difco agar, BD diagnostics). The bacterial stock solution was prepared by growing the culture in nutrient broth (NB) (5 g L⁻¹NB, 2 g L⁻¹NaCl) with shaking at 150 rpm at room temperature for 24 hours. The bacteria cells were then harvested by centrifugation at 4000×g at 4° C. for 30 minutes. The pellets were subsequently washed and suspended in NaCl solution (2 g L⁻¹, the same concentration as the experimental condition) to achieve an optical density (OD600) of 0.1.

The stock solution of dead bacteria was prepared using the above procedure followed by heating in 80° C. for 2 hours to ensure that the bacteria were killed. Viable bacterial counts were carried out for the dead bacterial stock and no colonies were found to be formed on the plates even after 36 hours of incubation at 37° C.

US11192069B2. Method and apparatus for assessing a state of fouling of a reverse osmosis system (2021-12-07). Nanyang Technological University [SG]. Inventors: Hans Gerard Leonard Coster [SG], Anthony Gordon Fane [SG], Lee Nuang Sim [SG], Jia Shin Ho [SG], Jiun Hui Low [SG].

+ Open protocol

+ Expand

Soft Agar Colony Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Difco™ Agar (BD Biosciences, Erembodegem, Belgium) was used in the assay, 1000 cells per well were added in top agar and were cultured for 2 weeks. Colonies (⩾50 cells/colony) were counted.

Yang S., Liu Y., Li M.Y., Ng C.S., Yang S.L., Wang S., Zou C., Dong Y., Du J., Long X., Liu L.Z., Wan I.Y., Mok T., Underwood M.J, & Chen G.G. (2017). FOXP3 promotes tumor growth and metastasis by activating Wnt/β-catenin signaling pathway and EMT in non-small cell lung cancer. Molecular Cancer, 16, 124.

+ Open protocol

+ Expand

Visualizing Bacterial Cell Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Copper grids (400 mesh, 62 μm pitch; Sigma-Aldrich) were placed with the Formvar side down, for 10 sec on a 7-day-old culture of Lcr growing on BM7A medium containing 0.25% or 0.75% agar (w/v; Difco^™ Agar, BD diagnostics, VWR, Radnor, PA, USA). Grids were carefully lifted off and the adherent bacterial cells were fixed/stained in a drop of 2% (v/v) uranyl acetate for 60 sec. Excess uranyl acetate was then blotted off and the residue allowed to air dry. Grids were viewed at 100 kV accelerating voltage using a transmission electron microscope (Hitachi H-7000, Hitachi High-Technologies Corporation, Tokyo, Japan) equipped with a Veleta (2k×2k) CCD side mount camera.

Cai L., Jain M., Sena-Vélez M., Jones K.M., Fleites L.A., Heck M, & Gabriel D.W. (2021). Tad pilus-mediated twitching motility is essential for DNA uptake and survival of Liberibacters. PLoS ONE, 16(10), e0258583.

+ Open protocol

+ Expand

Yeast Strain Derivation and Culturing

Check if the same lab product or an alternative is used in the 5 most similar protocols

All Saccharomyces cerevisiae strains are derived from JKM 179 (57 (link)). Wild-type MATaade-1 leu2–3, 112 lys5 trp1::hisG ura3–52, hml::ADE1, hmr::ADE2, ade3::GALHO, and either rad50Δ::URA3 or nej1Δ::KanMX6. Yeast culturing and plating was done using YPAD broth or agar. YPAD was prepared in ddH₂O with 1% (w/v) Bacto-yeast extract (BD Biosciences, #212750), 2% (w/v) dextrose (Sigma, #D1912), 2% (w/v) Bacto peptone (BD Biosciences, #211677), and supplemented with 25 μg/ml adenine (Sigma, #A8626), with 2% (w/v) Difco agar (BD Biosciences, #214530) for plates. Yeast cultures were grown overnight in 1 ml of YPAD media in a rotary shaker at 30°C and 220 rpm.

Stanley F.K., Berger N.D., Pearson D.D., Danforth J.M., Morrison H., Johnston J.E., Warnock T.S., Brenner D.R., Chan J.A., Pierce G., Cobb J.A., Ploquin N.P, & Goodarzi A.A. (2020). A high-throughput alpha particle irradiation system for monitoring DNA damage repair, genome instability and screening in human cell and yeast model systems. Nucleic Acids Research, 48(19), e111.

+ Open protocol

+ Expand

Soft Agar Colony Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were suspended in a soft agar medium containing 0.33% Difco agar (BD Biosciences) in 2x EMEM medium (Lonza) supplemented with L-glutamine, antibiotics and 15% FBS, and plated onto 6-well plates over a layer of 0.5% soft agar medium. Cells were cultured under standard cell culture conditions and were fed with 0.2 mL of the regular medium twice a week. Plates were observed under a light microscope after 2 weeks, and colonies exceeding 50 μm in diameter were scored. Experiments were performed in triplicates; at least 5 fields of view for each replicate were evaluated.

Voronkova M.A., Luanpitpong S., Rojanasakul L.W., Castranova V., Dinu C.Z., Riedel H, & Rojanasakul Y. (2017). SOX9 Regulates Cancer Stem-Like Properties and Metastatic Potential of Single-Walled Carbon Nanotube-Exposed Cells. Scientific Reports, 7, 11653.

+ Open protocol

+ Expand

Soft Agar Colony Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pre-warmed 0.8% bottom agar (Difco™ Agar) (BD, Franklin Lakes, NJ, USA) was prepared with PBS. 1 mL of bottom agar was poured into each well in 12-well plate. Pre-warmed 0.4% top agar (BD) was prepared with culture medium for each cell type. After bottom agar was congealed, 1mL of 0.4% top agar (BD) containing 500 cells of U87MG or 1,000 cells of ahMNCs or hTERT-ahMNCs was poured on the bottom agar. After top agar is congealed, wells were incubated at 37°C and 5% CO₂ for 2 weeks. The medium was changed every 3 days.

Lee K.H., Nam H., Jeong D.E., Kim S.S., Song H.J., Pyeon H.J., Kang K., Hong S.C., Nam D.H, & Joo K.M. (2016). Sensitive Tumorigenic Potential Evaluation of Adult Human Multipotent Neural Cells Immortalized by hTERT Gene Transduction. PLoS ONE, 11(7), e0158639.

+ Open protocol

+ Expand

Arabidopsis-Colletotrichum Co-cultivation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Co-cultivation of C. tofieldiae and A. thaliana was performed as previously described (Frerigmann et al. 2021 ). Therefore, fungi and plants were co-cultivated on half-strength Murashige and Skoog medium (750 µM MgSO₄, 625 or 100 µM KH₂PO₄, 10.3 mM NH₄NO₃, 9.4 mM KNO₃, 1.5 mM CaCl₂, 55 nM CoCl₂, 53 nM CuCl₂, 50 µM H₃BO₃, 2.5 µM KI, 50 µM MnCl₂, 520 nM Na₂MoO₄,15 µM ZnCl₂,75 µM Fe-EDTA, and 500 µM morpholineethanesulfonic acid-KOH, pH 5.5) with either high Pi (625 µM Pi) or low Pi (100 µM Pi) concentration. The medium was supplemented with 1% nutrient-poor granulated Difco Agar (BD Biosciences). C. tofieldiae was pregrown on PDA media at 25 °C for at least one day and fungal suspension (50 mg/ml) was prepared as previously described (Frerigmann et al. 2021 ). For co-cultivation, stratified sterile Arabidopsis seeds were resuspended in 250 µl sterile water and 20 µl fungal suspension was added. After washing two times with 1 ml of 20 mM MgCl₂, infected seeds were transferred to high or low Pi plates. For each condition four to five replicate plates with seven seeds per plate were prepared. Plates were placed into a phytochamber and plants were grown at short-day conditions (10 h at 21 °C and 14 h at 19 °C).

Zimmermann S.E., Blau S., Frerigmann H, & Krueger S. (2021). The phosphorylated pathway of serine biosynthesis is crucial for indolic glucosinolate biosynthesis and plant growth promotion conferred by the root endophyte Colletotrichum tofieldiae. Plant Molecular Biology, 107(1-2), 85-100.

+ Open protocol

+ Expand

Chemotaxis Assay Protocol for C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemotaxis assays were performed essentially as previously described (82 (link)). Briefly, assay plates were prepared the day of the assay by adding 10 mL of 2% Difco-agar (BD) in assay buffer (5 mM potassium phosphate, pH 6.0, 1 mM CaCl₂, and 1 mM MgSO₄) to 10-cm round petri dishes. After agar was solidified, 1 μL of 1M sodium azide was placed at opposite ends of the assay plate and allowed to soak in. Young adult hermaphrodites were washed off their growth plates with cholesterol-free S Basal into 1.5-mL microcentrifuge tubes and allowed to settle to the bottom. The wash step was repeated twice with S-Basal before animals were resuspended in Milli-Q water, and approximately 200 worms were placed at the origin, which was equidistant from two sodium azide spots. One microliter of the odorant (benzaldehyde, Sigma) dissolved in ethanol (200 proof, Sigma) or solvent control (ethanol) were added to the sodium azide spots, the petri-dish lid was closed, and animals within a 2-cm radius of the odorant and solvent spots were counted 60 minutes later. Chemotaxis index (CI) was calculated as follows:
Assays were performed in triplicate with all genotypes tested in parallel and repeated on at least three separate days.

Campagna C.M., McMahon H, & Nechipurenko I. (2023). The G protein alpha Chaperone and Guanine-Nucleotide Exchange Factor RIC-8 Regulates Cilia Morphogenesis in Caenorhabditis elegans Sensory Neurons. bioRxiv.

+ Open protocol

+ Expand

Microbial Identification and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Difco™ agar and Luria broth (LB) culture medium were obtained from BD Biosciences (Franklin Lakes, NJ, USA). The polymerase chain reaction (PCR) primers for 16S ribosomal (r)RNA and DNA gyrase, subunit B (gyrB) genes were synthesized by MD Bio (Taipei, Taiwan). The API^® 50 CHB system assay kit was obtained from bioMerieux (Marcy-l'Étoile, France). Bond Elut^® C18 was purchased from Agilent Technologies (Santa Clara, CA, USA). The polytetrafluoroethylene membrane (JP020) was obtained from Advantec Co., Ltd. (Tokyo, Japan). The indicator strains Staphylococcus epidermidis and Bacillus cereus were obtained from Bioresource Collection and Research Center (Hsinchu, Taiwan). The indicator strains Streptococcus pyogenes, Listeria monocytogenes, Clostridium tyrobutyricum, Escherichia coli, Helicobacter pylori, Salmonella typhimurium, Pseudomonas aeruginosa, Aspergillus flavus var. flavus, Aspergillus niger and Penicillium pinophilum were obtained from American Type Culture Collection (Manassas, VA, USA). MRSA HCT20 was kindly provided by the Department of Pathology and Laboratory Medicine, Taichung Veterans General Hospital (Taichung, Taiwan). The iturin A and surfactin standards were purchased from Sigma-Aldrich; Merck Millipore (Darmstadt, Germany).

Chen J.N., Wei C.W., Liu H.C., Chen S.Y., Chen C., Juang Y.M., Lai C.C, & Yiang G.T. (2016). Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Molecular Medicine Reports, 14(6), 5155-5163.

+ Open protocol

+ Expand

Cariogenic Streptococcus mutans Biofilm Growth Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SM was acquired from American Type Culture Collection
(ATCC Catalog#25175, Manassas, VA, USA). This
ATCC strain of SM has been isolated from carious dentin and has been
extensively used in dental caries research.^{24 (link),27} BBL^™ Brain heart infusion
(BHI) media and Difco^™ agar were acquired
from BD Biosciences (San Jose, CA, USA). Sucrose for cell growth was
acquired from MP Biomedicals, LLC (Irvine, CA, USA). Phosphate-buffered
saline (PBS, 1x, phosphate buffer 10 mM, potassium chloride 2.7
mM, sodium chloride 137 mM, pH 7.4) was used from Bioland Scientific
(Paramount, CA, USA). SM biofilms were grown for 24hours. BHI liquid culture
was inoculated with an isolated SM colony. The liquid culture was diluted
1:50 in BHI media (1.0%w/v sucrose) in each well in a 96-well plate
containing either control or AHA substrates. The plate was then incubated in
the dark at 37°C and 5.0% carbon dioxide for 24hours. The growth
curves generated were obtained by seeding an overnight liquid culture of SM
(5.6×10⁸±1.4×10⁸ CFUs/mL) at
a 1:50 dilution in fresh BHI and 1% Sucrose on 1.5, 2.0wt.% or control
substrate. The optical density of these cultures was monitored at
37°C under 5% CO2 with a Biotek Synergy H1 Microplate reader with Gen
5^™ Microplate Data Collection and Analysis software
(Thermo Fisher Scientific^™, Pittsburgh, PA, USA) over a
24hour interval to observe SM growth and proliferation.

Mori D.I., Powell A., Kehe G.M., Schurr M.J., Nair D.P, & Puranik C.P. (2021). Acrylated hydroxyazobenzene copolymers in composite-resin matrix inhibits S. mutans biofilms in vitro. Pediatric dentistry, 43(6), 484-491.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!