Methylglyoxal (MG), propidium iodide, [3(4,5-dimethylthi-azol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT), neutral red, carbenoxolone disodium salt (CBX), aminoguanidine hemisulfate salt (AG), standard glutathione, o-phthaldialdehyde, metaphosphoric acid, L-glutamate, N-methyl-D-glucamine, 4-(2-hydroxyethyl) piperazine-L-ethanesulfonic acid (HEPES), and cell culture materials were purchased from Sigma (Saint Louis, MO, USA). Dulbecco's modified Eagle medium (DMEM) and Dulbecco's phosphate-buffered saline (DPBS) were purchased from Gibco BRL (Carlsbad, CA, USA). Fetal bovine serum was obtained from Cultilab (Campinas, SP, Brazil), and L-[2,3-3H] glutamate was purchased from Amersham International (United Kingdom). Polyclonal anti-EAAT1 (GLAST) and anti-EAAT2 (GLT-1) were purchased from Abcam (Cambridge, MA, USA), anti-EAAT3 was purchased from Novus Biologicals (Littleton, CO, USA), and polyclonal anti-glyoxalase 1 was purchased from Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). All other chemicals were purchased from local commercial suppliers.
Carbenoxolone disodium salt
Manufactured by Merck Group
Sourced in United States
Carbenoxolone disodium salt is a laboratory reagent used in various research applications. It is a sodium salt derivative of carbenoxolone, which is a synthetic compound with potential pharmacological properties. The core function of this product is to serve as a research tool for investigating biological processes and molecular interactions in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Bicuculline methiodide, by Merck Group (2 mentions)
Probenecid, by Thermo Fisher Scientific (2 mentions)
Hbss 1x, by Thermo Fisher Scientific (2 mentions)
Metaphosphoric acid, by Merck Group (1 mentions)
Cell culture material, by Merck Group (1 mentions)
Aminoguanidine hemisulfate salt, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Cultilab (1 mentions)
Standard glutathione, by Merck Group (1 mentions)
Neutral red, by Merck Group (1 mentions)
L glutamate, by Merck Group (1 mentions)
N methyl d glucamine, by Merck Group (1 mentions)
4 2 hydroxyethyl piperazine l ethanesulfonic acid hepes, by Merck Group (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
O phthaldialdehyde, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Methylglyoxal, by Merck Group (1 mentions)
Apyrase, by Merck Group (1 mentions)
Brilliant blue g, by Merck Group (1 mentions)
Mrs2179, by Merck Group (1 mentions)
12 protocols using carbenoxolone disodium salt
1
Methylglyoxal Toxicity Assay Protocol
2
Antagonists of P2 Purinergic Receptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
P2 receptor antagonists: Pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid) tetrasodium salt hydrate (PPADS), MRS2211, MRS2179, MRS2159, A438079, Brilliant Blue G, ATP-2′, 3′-dialdehyde (OxATP), niflumix acid, carbenoxolone disodium salt, and apyrase were purchased from Sigma-Aldrich (St. Louis, MO, United States). NF449, NF023, and Evans Blue tetrasodium salt were purchased from Tocris Bioscience (Abingdon, United Kingdom). Suramin hexasodium salt was purchased from Aladdin (Shanghai, China). Anti-His monoclonal antibody was purchased from EARTHOX Life Sciences (Millbrae, CA, United States). Anti-ETX monoclonal antibody was developed previously by colleagues in our laboratory. HRP-coupled goat anti-mouse IgG antibody was purchased from TransGen Biotech (Beijing, China). The ATP assay kit was purchased from Calbiochem-Merck (Darmstadt, Germany). MTS (3-(4, 5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl) -2-(4-sulfopheny)-2H-tetrazolium, inner salt) was purchased from Promega Corporation (Madison, WI, United States).
3
Cyclophosphamide Metabolism Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cyclophosphamide (CYP) was purchased from LKT laboratories, Inc., (St. Paul, MN, USA). Corn oil, 18α-glycyrrhetinic acid (GA) and carbenoxolone disodium salt (CBX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bay K8644 was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
4
Pharmacological Modulation of Neurotransmission
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbachol (carbamylcholine chloride), kainate (kainic acid monohydrate), quinpirole, (+)- sulpiride, (+)-SKF-38393, PD168,077, carbenoxolone disodium salt, picrotoxin were purchased from Sigma (Sigma-Aldrich, Buchs, Switzerland); SYM2206 and L745.870 were from Tocris Bioscience (Bristol, UK); AP-5 (DL-2-amino-5-phosphopentanoic acid) was from Alexis Biochemicals (San Diego, CA); R(+)-SCH23390 HCl was from Research Biochemical International (Sigma-Aldrich, Buchs, Switzerland).
5
HPLC Extraction of Glucocorticoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All HPLC grade solvents used in the extraction protocol and glucose-6-phosphate were obtained from Merck (Darmstadt, Germany). Ultrapure water was obtained using an Arium®-pro ultrapure system (Sartorius, Goettingen, Germany). Dimethyl sulfoxide (99.9%), cortisone, hydrocortisone, carbenoxolone disodium salt, and glucose 6-phosphate dehydrogenase were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mixed gender pooled human liver microsomes were purchased from Sekisui XenoTech (Kansas, KS, USA). NADP+/H was obtained from Cayman Chemical (Ann Arbor, MI, USA).
6
Carbenoxolone and Probenecid Stock Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbenoxolone disodium salt (≥98%; Sigma Aldrich) and water-soluble Probenecid (77 mg/ml; Invitrogen) were dissolved in Hanks’s Balanced Salt Solution (HBSS 1X, Life Technologies; calcium chloride, magnesium chloride, magnesium sulfate) to develop stock concentrations of each compound. Spironolactone was purchased from Selleckchem (#52-01-7).
7
Assessing Carbenoxolone and Probenecid Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbenoxolone disodium salt (≥98%; Sigma Aldrich) and water-soluble Probenecid (77 mg/mL; Thermo Fisher Scientific) were dissolved in Hanks’s Balanced Salt Solution (HBSS 1X, Gibco, Thermo Fisher Scientific); with calcium chloride, magnesium chloride, magnesium sulfate) to develop stock solutions of each compound. For in vitro assays, the blockers were added to culture medium only once, on day 0, whereas for chick-CAM assays, the blockers were applied once daily for 7 days. Final concentrations of 100 μM CBX or 1mM PBN were used for each assay.
8
Carbenoxolone Inhibits 11β-HSD Enzymes
9
Pharmacological Modulation of Olfactory Bulb Oscillations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were initially anesthetized using isoflurane and implanted with a guide-electrode complex in the left and right OB. Following implantation, isoflurane was substituted for KX anesthesia (see above for further information). LFPs were recorded bilaterally and when a stable fast oscillation was visible (80–130 Hz). NBQX (2 μg, Sigma, n = 7), Bicuculline methiodide (0.05 μg, Sigma, n = 5) and Carbenoxolone disodium salt (1 μg, Sigma, n = 4) were infused into the bulb. Saline was infused to the opposite bulb. For infusion, internal cannulae (28 gauge, Bilaney) that extended 2 mm below the tip of the guides were inserted for 60 s followed by 60 s infusion of NBQX, bicuculline, CBX or saline (volume 0.5 μl). Rats were recorded for up to 30 min post infusion.
10
Characterizing Apoptosis Pathway Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbenoxolone disodium salt was purchased from Sigma (St. Louis, MO, USA), and a 100 mmol/L stock solution was prepared in H2O. Probenecid was purchased from Sigma, and a 200 mmol/L stock solution was prepared in DMSO. The drugs used for the flow cytometry experiments were QVD-OPh (#OPH001, R&D Systems, Minneapolis, MN, USA, 10 mmol/L stock solution in DMSO), S63-845 (Chemietek, Indianapolis, IN, USA, 10 mmol/L stock solution in DMSO), and ABT737 (Selleckchem, Houston, TX, USA, 10 mmol/L stock solution in DMSO).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!