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Wyatt mp 030s5 hplc size

Manufactured by Wyatt Technology
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The Wyatt MP-030S5 is a high-performance liquid chromatography (HPLC) size-exclusion chromatography (SEC) system. It is designed to separate and analyze the size distribution of macromolecules and particles in solution.

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Lab products found in correlation

Akta micro, by GE Healthcare (2 mentions) Dawn 8, by Wyatt Technology (2 mentions) Optilab t rex, by Wyatt Technology (2 mentions)

2 protocols using wyatt mp 030s5 hplc size

1

Size-Exclusion Chromatography of Ctf4 Protein

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Around 100 μg of Ctf4 FL protein was loaded onto a Wyatt MP-030S5 HPLC size-exclusion chromatography column (Wyatt) mounted on an AKTA Micro (GE Healthcare) chromatography. The column was equilibrated in a buffer containing 150 mM NaCl, 20 mM Tris pH 8.0, 1 mM DTT. The chromatography system was coupled to an 8-angle light scattering detector (DAWN 8+) and a refractive index detector (OptiLab TRex) (Wyatt Technology). Data were collected every 0.5 seconds. Data analysis was carried out using ASTRA VI.
Simon A.C., Zhou J.C., Perera R.L., van Deursen F., Evrin C., Ivanova M.E., Kilkenny M.L., Renault L., Kjaer S., Matak-Vinković D., Labib K., Costa A, & Pellegrini L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome. Nature, 510(7504), 293-297.
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2

Size-Exclusion Chromatography of Ctf4 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Around 100 μg of Ctf4 FL protein was loaded onto a Wyatt MP-030S5 HPLC size-exclusion chromatography column (Wyatt) mounted on an AKTA Micro (GE Healthcare) chromatography. The column was equilibrated in a buffer containing 150 mM NaCl, 20 mM Tris pH 8.0, 1 mM DTT. The chromatography system was coupled to an 8-angle light scattering detector (DAWN 8+) and a refractive index detector (OptiLab TRex) (Wyatt Technology). Data were collected every 0.5 seconds. Data analysis was carried out using ASTRA VI.
Simon A.C., Zhou J.C., Perera R.L., van Deursen F., Evrin C., Ivanova M.E., Kilkenny M.L., Renault L., Kjaer S., Matak-Vinković D., Labib K., Costa A, & Pellegrini L. (2014). A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome. Nature, 510(7504), 293-297.
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