Azoxymethane (AOM) was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dextran sodium sulfate (DSS) was purchased from MP Biomedicals (Santa Ana, CA, USA). Antibodies of anti-P53, anti-gamma H2AX (phospho S139), anti-OXoguanine8, and anti-PCNA were purchased from Abcam (Cambridge, UK). Goat anti-mouse IgG (H+L) and goat anti-rabbit IgG (H+L) were purchased from Jackson Immuno-Research (West Grove, PA, USA). The Beta actin antibody was obtained from ZSGB-BIO (Beijing, China). In situ cell death detection kits, POD, were obtained from Roche Ltd. (Laval, QC, Canada). Phosphate buffer saline was obtained from Amresco (Solon, OH, USA). Liquid paraffin and neutral formalin were purchased from Beijing Chemical Works (Beijing, China).
Beta actin antibody
Manufactured by ZSGB-BIO
Sourced in China
The Beta actin antibody is a primary antibody used to detect the presence of the beta actin protein, a highly conserved cytoskeletal protein found in all eukaryotic cells. It serves as a reliable loading control in western blot analyses to normalize protein expression levels across samples.
Automatically generated - may contain errors
Lab products found in correlation
Neutral formalin, by Beijing Chemical Works (1 mentions)
Goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions)
Azoxymethane aom, by Merck Group (1 mentions)
Goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions)
Liquid paraffin, by Beijing Chemical Works (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Anti pcna, by Abcam (1 mentions)
Cell lysis kit, by Bio-Rad (1 mentions)
Rip1 antibody, by Abcam (1 mentions)
Rip3 antibody, by Abcam (1 mentions)
2 protocols using beta actin antibody
1
Azoxymethane and Dextran Sulfate Sodium Colorectal Cancer Model
2
Western Blot Analysis of Necroptosis Proteins
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Tissue (30 mg) was rapidly sampled from the harvest flap on ice. Protein was extracted through the tissue with a cell lysis kit (Bio-Rad Laboratories, Hercules, CA). The protein samples were electrophoresed using 10% SDS-PAGE for 1.5 h at 100 V. Following transfer to nitrocellulose membranes, blots were blocked with BSA for 1.5 h. Beta Actin antibody (1:1000, ZSGB-BIO, Beijing, China), RIP1 antibody (1:1000, Abcam, Cambridge, Britain), and RIP3 antibody (1:1000, Abcam, Cambridge, Britain) were incubated overnight at 4 °C in blocking buffer. Blots were washed three times in TBS + 0.05% Tween, followed by incubation with secondary antibodies (1:10000, LI-COR, Lincoln, NE) in the dark for 1 h at room temperature. Immunoblots were read on an Odyssey Imager (Odyssey, Lincoln, NE).
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