PBEA was standardized with Eugenol by gas chromatography with flame ionization detector (Perkin Elmer GC clarus 500) and analyzed by TotalChrom Navigator clarus 500 software. Instrument is coupled with a split/splitless injector, run in a split-mode and ZB-5 capillary column (cross bond 5% diphenyl/95% dimethyl polysiloxane, 30 m × 250 μm) was used throughout the analysis. The GC-FID parameters were used in the analysis on the basis of the boiling point and affinity towards the stationary phase of the drug. Eugenol has a boiling point of about 254 °C. 1 μL sample was manually injected at an inlet temperature and detector temperature of 240 °C and 300 °C, respectively. After injection, the oven temperatures was increased quickly from 60 °C and hold for 2 min then programmed within 25min–310 °C at a rate of 10 °C per min and hold for 8 min. Carrier gas nitrogen (0.942 mL/min), synthetic air (450 mL/min), hydrogen (45 mL/min) were fed to the FID.
Clarus 500 gc
Manufactured by PerkinElmer
Sourced in United States
The Clarus 500 GC is a gas chromatography system designed for analytical applications. It is capable of performing precise and reliable separations of complex mixtures. The Clarus 500 GC utilizes advanced technology to provide accurate and reproducible results.
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Lab products found in correlation
Clarus 500 mass spectrometer, by PerkinElmer (4 mentions)
Porapak q 1 8, by Teknokroma (4 mentions)
Aoc 20i, by PerkinElmer (3 mentions)
Elite 5ms, by PerkinElmer (2 mentions)
Turbo mass gold, by PerkinElmer (2 mentions)
Aoc 20i autosampler, by PerkinElmer (2 mentions)
Elite 5ms capillary column, by PerkinElmer (2 mentions)
Turbomatrix 110, by PerkinElmer (1 mentions)
Elite 1 fused silica capillary column, by PerkinElmer (1 mentions)
Jms t100 gcv, by JEOL (1 mentions)
Db 5ms column, by Agilent Technologies (1 mentions)
Lambda 35 uv vis spectrophotometer, by PerkinElmer (1 mentions)
Zb 5msi column, by Phenomenex (1 mentions)
Clarus 500, by PerkinElmer (1 mentions)
Stabilwax capillary column, by Restek (1 mentions)
Authentic compounds, by Merck Group (1 mentions)
Clarus 500 system, by PerkinElmer (1 mentions)
Totalchrom software, by PerkinElmer (1 mentions)
Elite 225 column, by PerkinElmer (1 mentions)
Elite ffap column, by PerkinElmer (1 mentions)
42 protocols using clarus 500 gc
1
Standardization of PBEA by GC-FID
2
GC-MS Analysis of Epaltes divaricata L.
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GC-MS analysis of the ethyl acetate extracts found to be better than other solvents of Epaltes divaricata L. was performed using a perkin Elmer GC clarus 500 system comprising AOC-20i auto-sampler and a Gas chromatograph interfaced to a Mass spectrometer (GC-MS) equipped with a Elite-5MS (5% Diphenyl/95% Dimethyl Poly Siloxane) fused capillary column (30 × 0.25 μm 1D × 0.25 μm dF). For GC-MS detection, an electron ionization system was operated in electron impact mode with ionization system operated in electron impact mode with ionization energy of 70ev. Helium gas (99.999%) was used as carrier gas at a constant flow rate of 1 ml/min, and an injection volume of 2 μl was employed (split ratio of 10:1). The relative percentage amount of each component was calculated by comparing its average peak area to the total areas. The mass detector used in this analysis was Turbo-Mass Gold-Perkin Elmer and the software adopted to handle mass spectra and chromatograms was a Turbo-Mass ver 5.2. GC-MS was conducted using the database of central electrochemical research institute characterization and measurement laboratory having more than 62,000 patterns. The spectrum of the unknown components was compared with the spectrum of known components stored in the data bank, Central Electrochemical Research Institute Characterization and Sargam metals, Chennai, India.
3
GC-MS Analysis of Compound Extracts
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Gas Chromatography-mass spectrometry(GC-MS) is used in identification of several compounds including volatile, non-volatile and thermally stable compounds. Dried extract of CE was dissolved in 95% v/v methanol and analyzed using GC Clarus 500, PerkinElmer, USA and equipped with Turbo mass gold-pekin Elmer Detector and split injection system. 2µl of sample was injected for analysis and the analysis was carried out.The unknown component was compared to NIST library (Madhuri et al., 2009).
4
GC-MS Analysis of Plant Samples
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GC-MS of the plant samples was performed using a PerkinElmer GC Clarus 500 system and chromatograph coupled to a mass spectrometer equipped with an Elite-I, fused silica capillary column (30 mm 90.25 mm ID 91 IMdf, consisting of 100% dimethyl polysiloxane). The sample was dissolved in n-hexane and GC-MS identification was operated in electron impact mode with an ionization energy of 70 eV. The National Institute of Standard and Technology (NIST) library database was used for the interpretation of the compounds detected on a mass spectrum (GC-MS) through which the weighed, molecular formula and structural formula of the samples were determined (55 (link)).
5
Chromatographic Analysis of Biodegraded Crude Oil
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Extraction of crude oil carried out according to pervious method, and alterations in its chemical composition were studied by chromatographic analysis, such as gas chromatography-mass spectrometry (GC-MS). The oil extracted from the biodegraded crude oil was monitored using a Perkin–Elmer GC Clarus 500 system (PerkinElmer: Boston, MA, US) flame ionization detector. Crude oil was separated using a capillary column (30 m × 0.25 mm × 0.25 µm) under the conditions described in a previous study [25 (link)]. The inlet temperature program was 50 °C/min. The initial temperature of the oven was maintained at 60 °C for 2 min and increased linearly at a rate of 6 °C/min and was maintained at a final temperature of 300 °C. The operating temperatures of the injector and detector were 300 °C and 320 °C, respectively. The detector temperature was 300 °C. Then, 1 µL of sample was injected with a 1:50 split ratio, and the total run time was less than 35 min.
6
GC-MS Analysis of Bioactive Extract
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GC-MS analysis of bioactive extract was carried out on GC Clarus 500 Perkin Elmer system comprising AOC-20i auto sampler. The spectrums of unknown components present in the bioactive extract were identified by compared with known components spectrum which stored in the NIST and WILEY libraries. The name, molecular weight and structure of the components present in the bioactive extract were ascertained. The GC-MS analysis was carried out at Sophisticated Analytical Instrument Facility (SAIF), Indian Institutes of Technology, Chennai, India.
7
Quantifying Acetaldehyde in Biological Samples
8
GC-MS Analysis of CQ Extract
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GC/MS analysis was carried by employing 2 μl of the CQ extract. Gas chromatograph interfaced to a mass spectrometer (GC-MS) analysis was performed on a GC clarus 500 Perkin Elmer system comprising a AOC-20i autosampler and GC-MS instrument employing the following conditions: Column elite-1 fused silica capillary column (30 × 0.25 mm ID × 1 EM df, composed of 100% dimethyl poly siloxane), operating in electron impact mode at 70 eV, helium (99.999%) was used as carrier gas at a constant flow of 1 ml/min and an injection volume of 0.5 EI was employed (split ratio of 10:1) injector temperature 270°C, ion-source temperature 230°C. The oven temperature was programmed from 110°C (isothermal for 2 min), with an increase of 10°C/min, to 200°C/min, then 5°C/min to 280°C/min, ending with a 9 min isothermal at 280°C. Mass spectra were taken at 70 eV; a scan interval of 0.5 s and fragments from 40 to 550 Da.
9
GC-MS Analysis of Chromatographic Fractions
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GC-MS analysis was carried out on column chromatographic fractions. The analysis of the fractions and formulations was performed using the GC Clarus 500 Perkin Elmer system comprising an AOC-20i auto sampler and gas chromatographer interfaced to a mass spectrometer instrument equipped with an Elite-5MS fused capillary column (length: 300 m, diameter: 0.25 m, and film thickness: 0.25 μm). For GC-MS detection, an electron ionization energy of 70 eV was used. Helium (99.9%) was used as the carrier gas at a constant flow rate of 30 cm/second, and an injection volume of 3 μL was employed (split ratio: 10:1). The injector and ion-source temperature were 250 and 280 °C, respectively. The oven temperature was programmed to start at 110 °C (isothermal for 2 min), and then increase at 10 °C/min to 200 °C, before changing at 5 °C/minto 280 °C, ending with an isothermal condition at 280 °C for the acquisition of mass spectra and chromatograms with a Turbomass 5.2 (PerkinElmer, Inc., Shelton, CT, USA).
10
GC-MS Analysis of Volatile Compounds
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GC-MS analysis was carried out on a GC clarus 500 Perkin Elmer system (USA) comprising a AOC-20i auto sampler and gas chromatograph interfaced to a mass spectrometer instrument employing the following conditions: column Elite-1 fused silica capillary column (30 × 0.25 mm ID × 1μ Mdf, composed of 100% Dimethyl polysiloxane), operating in electron impact mode at 70 eV; Helium gas (99.999%) was used as carrier gas at a constant flow of 1 mL/min and an injection volume of 0.5 μl was employed (split ratio of 10:1) injector temperature 250 °C, ion-source temperature 280 °C. The oven temperature was programmed from 110 °C (isothermal for 2 min), with an increase of 10 °C/min, to 200 °C, then 5 °C/min to 280 °C, ending with a 9 min isothermal at 280 °C. Mass spectra were taken at 70 eV; a scan interval of 0.5 s and fragments from 40 to 450 Da. Total GC running time is 36 min. The relative percentage amount of each component was calculated by comparing its average peak area to the total areas. TurboMass Ver 5.2.0 software was employed to handle mass spectra and chromatograms.18
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