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6220 lc tof ms

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The Agilent 6220 LC-TOF-MS is a high-resolution time-of-flight mass spectrometer designed for liquid chromatography-mass spectrometry (LC-MS) applications. It provides accurate mass measurements and high-speed data acquisition for a wide range of analytes.

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Lab products found in correlation

P 2000 polarimeter, by Jasco (9 mentions) Uv 1201 spectrophotometer, by Shimadzu (6 mentions) Avance 500 spectrometer, by Bruker (5 mentions) Spd m10a, by Shimadzu (3 mentions) Avance 500 or 600 spectrometers, by Bruker (3 mentions) M series ftir, by Shimadzu (2 mentions) J 815 spectrometer, by Jasco (2 mentions) J 815 circular dichroism spectrometer, by Jasco (2 mentions) Advance 600 spectrometer, by Bruker (1 mentions) J 810 spectropolarimeter, by Jasco (1 mentions) Lc 10at, by Phenomenex (1 mentions) Avance 500, by Bruker (1 mentions) Lc 10at, by Shimadzu (1 mentions)

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6220 TOF-MS (7 citations) TOF LC/MS (2 citations) 6220 oa-TOF LC/MS mass spectrometer (2 citations) 6220 Accurate-Mass TOF LC/MS (2 citations) 6220 oa-TOF LC/MS spectrometer (2 citations) 6220 Accurate-Mass TOF LC/MS system (2 citations)

10 protocols using 6220 lc tof ms

1

NMR and Mass Spectrometry Analysis

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1H and 13C NMR spectra were recorded using a Bruker Advance 600 spectrometer. Mass spectra were obtained with an Agilent 6220 LC-TOF-MS. Semi-preparative HPLC was performed using Shimadzu LC-10AT pumps coupled with a Sedex 75 Evaporative Light Scattering Detector (ELSD), a Shimadzu SPD M10A diode array detector, a SCL-10A system controller, and a Cogent Bidentate C18 column (250 × 10 mm or 75 × 4.6 mm).
Eaton A.L., Brodie P.J., Callmander M.W., Rakotondrajaona R., Rakotobe E., Rasamison V.E, & Kingston D.G. (2015). Bioactive Oleanane Glycosides from Polyscias duplicata from the Madagascar Dry Forest []. Natural product communications, 10(4), 567-570.
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2

Comprehensive Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2000 polarimeter. IR and UV spectra were measured on MIDAC M-series FTIR and Shimadzu UV-1201 spectrophotometers, respectively. ECD analysis was performed on a JASCO J-810 spectropolarimeter with a 0.1cm cell in MeOH at room temperature under the following conditions: speed 50 nm/min, time constant 1 s, band width 2.0 nm. 1H and 13C NMR spectra were recorded on a Bruker Avance 500 spectrometer in CDCl3 with TMS as internal standard. Mass spectra were obtained on JEOL JMS-HX-110 and Agilent 6220 LC-TOF-MS. Preparative HPLC was performed using Shimadzu LC-10AT pumps coupled with a semi-preparative Varian Dynamax C18 column (5 μm, 250x10 mm), a Shimadzu SPD M10A diode array detector (DAD) and a SCL-10A system controller.
Rakotondraibe L.H., Graupner P.R., Xiong Q., Olson M., Wiley J.D., Krai P., Brodie P.J., Callmander M.W., Rakotobe E., Ratovoson F., Rasamison V.E., Cassera M.B., Hahn D.R., Kingston D.G, & Fotso S. (2015). Neolignans and Other Metabolites from Ocotea cymosa from the Madagascar Rain Forest and their Biological Activities,. Journal of natural products, 78(3), 431-440.
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3

Spectroscopic Characterization of Compounds

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV and IR spectroscopic data were measured on a Shimadzu UV-1201 spectrophotometer and a MIDAC M-series FTIR spectrophotometer, respectively. ECD spectra were obtained on a JASCO J-815 spectrometer. NMR spectra were recorded in CDCl3 on Bruker Avance 500 or 600 spectrometers. The chemical shifts are given in δ (ppm), and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode.
Du Y., Pearce K.C., Dai Y., Krai P., Dalal S., Cassera M.B., Goetz M., Crawford T.D, & Kingston D.G. (2017). Antiplasmodial Sesquiterpenoid Lactones from Trichospira verticillata: Structure Elucidation by Spectroscopic Methods and Comparison of Experimental and Calculated ECD Data. Journal of natural products, 80(5), 1639-1647.
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4

Spectroscopic Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV and IR spectroscopic data were measured on a Shimadzu UV-1201 spectrophotometer and a MIDAC M-series FTIR spectrophotometer, respectively. CD spectra were obtained on a JASCO J-815 circular dichroism spectrometer. NMR spectra were recorded in CDCl3 on Bruker Avance 500 or 600 spectrometers. The chemical shifts are given in δ (ppm), and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode.
Du Y., Abedi A.K., Valenciano A.L., Fernández-Murga M.L., Cassera M.B., Rasamison V.E., Applequist W.L., Miller J.S, & Kingston D.G. (2017). Isolation of the New Antiplasmodial Butanolide Malleastrumolide A from a Malleastrum sp. (Meliaceae) from Madagascar. Chemistry & biodiversity, 14(12), 10.1002/cbdv.201700331.
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5

Comprehensive Analytical Characterization

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Optical rotations were obtained using a JASCO P-2000 polarimeter. ECD spectra were acquired on JASCO J-815 spectrometer. UV spectra were measured on a Shimadzu UV-1201 spectrophotometer. NMR spectra were recorded on a Bruker Avance 500 spectrometer with deuterated solvent as indicated. High resolution electrospray ionization mass spectra (HRESIMS) were obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode. Unless otherwise stated, HPLC was carried out on a Phenomenex Luna 5μ C18 4.6 x 250 mm column at a flow rate of 1 mL/min.
Fuentes R.G., Pearce K.C., Du Y., Rakotondrafara A., Valenciano A.L., Cassera M.B., Rasamison V.E., Crawford T.D, & Kingston D.G. (2018). Phloroglucinols from the Roots of Garcinia dauphinensis and their Antiproliferative and Antiplasmodial Activities. Journal of natural products, 82(3), 431-439.
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6

Spectroscopic Analysis of Compound Characterization

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV spectra were measured on a Shimadzu UV-1201 spectrophotometer. IR spectra were measured on a MIDAC M-series FTIR spectrometer. NMR spectra were recorded in CDCl3 on a Bruker Avance 500 spectrometer. Chemical shifts are given in δ (ppm), and coupling constants are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode. Open column chromatographies were performed using Sephadex LH-20 (3 × 50 cm, 150 g) and C18 (2.5 × 10 cm, 40–63 µm, 45 g). Semi-preparative HPLC was performed on a Shimadzu LC-10AT instrument with a semipreparative C18 Varian Dynamax column (5 µm, 250 ×10 mm).
Su Q., Krai P., Goetz M., Cassera M.B, & Kingston D.G. (2015). Antiplasmodial Isoflavanes and Pterocarpans from Apoplanesia paniculata. Planta medica, 81(0), 1128-1132.
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7

Analytical Characterization of Compounds

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV and IR spectroscopic data were measured on a Shimadzu UV-1201 spectrophotometer and a MIDAC M-series FTIR spectrophotometer, respectively. CD spectra were obtained on a JASCO J-815 circular dichroism spectrometer. NMR spectra were recorded in CDCl3 on Bruker Avance 500 or 600 spectrometers. The chemical shifts are given in δ (ppm), and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS in the positive-ion mode.
Du Y., Valenciano A.L., Dai Y., Zheng Y., Zhang F., Zhang Y., Clement J., Goetz M., Kingston D.G, & Cassera M.B. (2019). Anibamine and its Analogues; Potent Antiplasmodial Agents from Aniba citrifolia. Journal of natural products, 83(3), 569-577.
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8

Comprehensive Characterization of Isolated Compounds

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IR and UV spectra were measured on MIDAC M-series FTIR and Shimadzu UV-1201 spectrophotometers, respectively. 1D and 2D NMR spectra were recorded on a Bruker Avance 500 spectrometer in CD3OD; chemical shifts are given in δ (ppm), and coupling constants are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF–MS in the positive ion mode. Optical rotations were recorded on a JASCO P-2000 polarimeter. Open column chromatography was performed using Sephadex LH-20 and silica gel (40–63 µm, Silicycle Co. USA). HPLC was performed on a Shimadzu LC-10AT instrument with a semipreparative C18 (Phenomenex Luna column, 5 µm, 250 Χ10 mm), a Shimadzu SPD M10A diode array detector, and a SCL-10A system controller. All isolated compounds were purified to 95 % purity or better, as judged by HPLC (both UV and ELSD detection) before determining bioactivity.
Su Q., Brodie P.J., Liu Y., Miller J.S., Andrianjafy N.M., Antsiferana R., Rasamison V.E, & Kingston D.G. (2016). Antiproliferative Triterpenoid Saponins from Leptaulus citroides Baill. from the Madagascar Rain Forest. Natural Products and Bioprospecting, 6(1), 31-39.
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9

Characterization of Chemical Compounds

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV
spectroscopic data were measured on a Shimadzu UV-1201 spectrophotometer. NMR
spectra were recorded in CDCl3 on Bruker Avance 500 or 600
spectrometers. Chemical shifts are given in δ (ppm), and
coupling constants (J) are reported in Hz. Mass spectra were
obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode.
Du Y., Martin B.A., Valenciano A.L., Clement J.A., Goetz M., Cassera M.B, & Kingston D.G. (2020). Galtonosides A-E: Antiproliferative and Antiplasmodial Cholestane Glycosides from Galtonia regalis. Journal of natural products, 83(4), 1043-1050.
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10

Analytical Techniques for Natural Product Isolation

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Optical rotations were recorded on a JASCO P-2000 polarimeter. UV spectra were measured on a Shimadzu UV-1201 spectrophotometer. IR spectra were measured on a MIDAC M-series FTIR spectrometer. NMR spectra were recorded in CDCl3 on a Bruker Avance 500 spectrometer. Chemical shifts are given in δ (ppm), and coupling constants are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS in the positive ion mode. Open column chromatographies were performed using Sephadex LH-20 (I.D.×L 3×50 cm) and Si-diol (I.D.×L 2.5 ×35 cm, 40–63 µm). HPLC separation was performed on a Shimadzu LC-10AT instrument with a semipreparative C18 Phenomenex Luna column (5 µm, 250 × 10 mm).
Su Q., Dalal S., Goetz M., Cassera M.B, & Kingston D.G. (2016). New Antiplasmodial Diterpenes from Gutierrezia sarothrae. Natural product communications, 11(6), 719-721.
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