Anti flag tag

Manufactured by BioLegend

The Anti-FLAG tag is a lab equipment product that recognizes and binds to the FLAG peptide sequence, a commonly used protein tag. It provides a reliable method for the detection and purification of FLAG-tagged proteins.

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Lab products found in correlation

Ab218810, by Abcam (1 mentions) Anti human cd3, by Abcam (1 mentions) Anti rabbit hrp, by Jackson ImmunoResearch (1 mentions) Anti rabbit biotin, by Jackson ImmunoResearch (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Mouse anti biotin, by Jackson ImmunoResearch (1 mentions) Anti mica, by R&D Systems (1 mentions) Anti ulbp 2 5 6, by R&D Systems (1 mentions) Anti mouse hrp, by Jackson ImmunoResearch (1 mentions) Anti ulbp1, by R&D Systems (1 mentions) Anti ulbp3, by R&D Systems (1 mentions) Anti micb clone 236511, by R&D Systems (1 mentions) Anti vinculin, by Abcam (1 mentions) Anti mica, by Abcam (1 mentions) Anti mouse alexafluor 647, by Jackson ImmunoResearch (1 mentions) Anti mouse pe, by Jackson ImmunoResearch (1 mentions) Streptavidin horseradish peroxidase hrp, by Jackson ImmunoResearch (1 mentions) Anti rat hrp, by Jackson ImmunoResearch (1 mentions) Anti rat 488, by Jackson ImmunoResearch (1 mentions) Anti rabbit cy3, by Jackson ImmunoResearch (1 mentions)

2 protocols using anti flag tag

Comprehensive Antibody Panel for Immunoanalysis

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The following primary antibodies were used for flow cytometry: anti-MICA (clone 159227; R&D Systems), anti-MICB (clone 236511, R&D Systems), anti-ULBP1 (clone 170818; R&D Systems), anti-ULBP2/5/6 (clone 165903; R&D Systems) and anti-ULBP3 (clone 166514, R&D Systems).
The following primary antibodies were used for immunofluorescence: anti-PDI (ab3672, Abcam), anti-FLAG tag (Clone L5, Biolegend) and anti-MICA (clone 159227, R&D Systems).
The following primary antibodies were used for western blotting: anti-MICA (Clone EPR6568, Abcam), anti-FLAG tag (Clone L5, Biolegend), anti-GAPDH (clone 6C5, Santa Cruz) and anti-vinculin (clone EPR8185, Abcam).
The following secondary antibodies were used: anti-mouse AlexaFluor 647, anti-mouse PE, anti-mouse biotin, anti-rabbit biotin, anti-rat biotin, anti-rabbit Cy3, anti-rat 488, streptavidin-AlexaFluor 647, streptavidin-horseradish peroxidase (HRP), anti-mouse-HRP, anti-rat-HRP and anti-rabbit-HRP, all purchased from Jackson Laboratories.

Seidel E., Dassa L., Schuler C., Oiknine-Djian E., Wolf D.G., Le-Trilling V.T, & Mandelboim O. (2021). The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing. PLoS Pathogens, 17(5), e1008807.

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Immunohistochemical and Immunofluorescence Analysis of Mouse Liver

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Mouse liver was fixed in formalin, embedded in paraffin, sectioned and stained with the indicated antibodies. Briefly, the slides were stained with anti-human HER2/ERBB2 (Cat #: 10004-T56, SinoBiological) or anti-human CD47 (ab218810, Abcam) for immunohistochemistry (IHC), and immunostained with anti-human CD3 (Cat #: ab11089, Abcam), anti-FLAG tag (Cat #: 637301, Biolegend) antibodies and DAPI for immunofluorescence, which performed by the immunoassay platform at the Shanghai Institute for Emerging and Re-emerging Infectious Diseases of SPHCC.

Liao Q., Liu Z., Zhu C., He H., Feng M., Jiang L., Ding X., Sun R., Zhang X, & Xu J. (2022). Rapid generation of a mouse model for evaluating on-target normal tissue toxicity of human CAR-T cells using replication-defective recombinant adenovirus. Journal of Advanced Research, 47, 163-171.

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