Agilent 6490 qqq

LC-MS/MS was obtained from Agilent 6490 QQQ (Agilent Technologies, Santa Clara, CA, USA) mass-spectrometer equipped with Agilent 1290 series Rapid Resolution LC system with Agilent Jet-Stream ESI interface (Agilent Technologies, Santa Clara, CA, USA). Mycotoxin separation was achieved on a reversed phase C18 column (ODS) (150 mm × 2.1 mm; 5 µm). Data acquisition, processing, and instrument control were performed through the Mass Hunter Workstation B.06.01 software. MS parameters were optimized by using mycotoxin mixture that contained 100 µg/L AFB1, AFG1, and OTA standards, and 30 µg/L of AFB2 and AFG2 standards in MeOH.

In the present study, sample concentrations from in vitro or in vivo pharmacokinetic studies were determined using LC-MS/MS in positive electrospray ionization mode. The LC-MS/MS system consisted of an Agilent 6490 QQQ with a 1290 Infinity HPLC system (Agilent Technologies, Santa Clara, CA, USA). To separate the analytes, SFN and SFN-NAC, from endogenous substances, a SynergiTM 4 μm polar RP 80A column (150 × 2.0 mm, Phenomenex, Torrance, CA, USA) was employed. The LC conditions were as follows: mobile phase, 0.1% formic acid and acetonitrile (50:50); flow rate, 0.2 mL/min; injection volume, 2 μL. Multiple reaction monitoring was achieved with m/z 178.1→114.1 for SFN, m/z 340.9→178.1 for SFN-NAC, and m/z 176.1→111.9 for an internal standard (sulforaphene). Data acquisition and processing were performed using the MassHunter software (version A.06.00, Agilent Technologies). The limit of quantitation was 0.2 ng/mL for SFN and 0.5 ng/mL for SFN-NAC.