Human mao a

Manufactured by Merck Group

Human MAO-A is a recombinant enzyme that catalyzes the oxidative deamination of monoamine neurotransmitters. It is used in research applications to study the metabolism and functions of these neurotransmitters.

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Lab products found in correlation

Human mao b, by Merck Group (1 mentions) Ampliflu red, by Merck Group (1 mentions) Mao b, by Merck Group (1 mentions) Filtermax f5, by Molecular Devices (1 mentions) Prism 5, by GraphPad (1 mentions)

2 protocols using human mao a

Kinetic Analysis of MAO-B Enzyme Inhibition

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The reagents and enzymes (Human MAO-B and Human MAO-A) applied in this experiment were purchased from Sigma-Aldrich. According to the manuals, all components (substrate solution and enzyme solution) are diluted and placed aside for use. The detailed process has been mentioned in our early work³⁴ (link).
To further examine the interaction mode of compound 17d, the type of enzyme inhibition was determined by Michealis–Menten kinetic experiments. The catalytic rates of MAO‐B enzyme were measured at six different concentrations of substrate p‐tyramine (0.05, 0.1, 0.25, 0.5, 1.0, and 1.25 mM) in the absence and in the three different concentrations (33.3, 100, and 300 nM) of compound 17d. The corresponding dose‐response curves and the nonlinear/linear regression analysis were performed using GraphPad Prism version 6 software (Graphpad Software Inc., La Jolla, CA).

Zhang C., Zhang Y., Lv Y., Guo J., Gao B., Lu Y., Zang A., Zhu X., Zhou T, & Xie Y. (2022). Chromone-based monoamine oxidase B inhibitor with potential iron-chelating activity for the treatment of Alzheimer’s disease. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 100-117.

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Fluorometric Assay for MAO-A and MAO-B

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Ampliflu™ Red (10-acetyl-3,7-dihydroxyphenoxazine), kynuramine, horseradish peroxidase, human MAO-A and MAO-B (expressed in insect cell membranes) were purchased from Sigma-Aldrich. human MAO-A from heterologous expression in yeast was purified as previously reported. 51 Compounds were dissolved in DMSO and stored in glass vials at 4°C in the dark. Control and inhibitor assays contained the same amount of DMSO (1% (v/v)). A multi-mode microplate reader (Molecular Devices FilterMax F5) was used for fluorometric assays with HRP and Ampliflu™ Red. A Uvikon 2101 spectrophotometer was used for absorbance assays with MAO-A and MAO-B. The IC 50 values for selected compounds were determined by plotting remaining activity of enzyme against the log of compound concentration. Data analysis was performed with GraphPad Prism 5 software using the three parameter equation.

Hroch L., Guest P., Benek O., Soukup O., Janockova J., Dolezal R., Kuca K., Aitken L., Smith T.K., Gunn-Moore F., Zala D., Ramsay R.R, & Musilek K. (2017). Synthesis and evaluation of frentizole-based indolyl thiourea analogues as MAO/ABAD inhibitors for Alzheimer's disease treatment. Bioorganic & medicinal chemistry, 25(3).

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