Samples from the obtained heart tissues were homogenized in 10% phosphate buffer to measure numerous biochemical parameters. ELISA kits were consumed to measure cardiac enzymes; creatine phosphokinase (CPK; ab187396), creatine phosphokinase heart (CK-MB; ab187396), cardiac troponin T (cTnT; ab246529), and cardiac troponin I (cTnI; ab246529); apoptotic markers; caspase 3 (ab39401), Caspase 9 (ab65608), and inflammatory mediators; tumor necrosis factor-alpha (TNF-α; ab46070), interleukin 6 (IL-6; ab100772), interleukin 1 beta (IL-1β; ab100768), and nuclear factor-κ-B (NFκB; ab176648) (all obtained from Abcam, Eugene, OR, USA), following the manufacturer’s protocols and using a microplate reader (SpectraMax i3x, Molecular Devices, USA).
Ab187396
Manufactured by Abcam
Sourced in United States
Ab187396 is a lab equipment product offered by Abcam. It is a device designed for use in scientific research applications. The core function of this product is to facilitate specific tasks or procedures within a laboratory setting. Detailed technical specifications and intended use are not provided in this response.
Automatically generated - may contain errors
Lab products found in correlation
Ab246529, by Abcam (2 mentions)
Elisa kit, by R&D Systems (1 mentions)
Ab65608, by Abcam (1 mentions)
Ab100768, by Abcam (1 mentions)
Ab100772, by Abcam (1 mentions)
Ab176648, by Abcam (1 mentions)
Ab46070, by Abcam (1 mentions)
Ab39401, by Abcam (1 mentions)
Spectramax i3x, by Molecular Devices (1 mentions)
Ab108667, by Abcam (1 mentions)
4 protocols using ab187396
1
Comprehensive Cardiac Biomarker Analysis
2
Myocardial Injury Biomarker Quantification
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Levels of creatine kinase-MB (CK-MB) (354798; USBiological, MA, Swampscott, USA), creatine kinase (CK) (ab187396; Abcam, Cambridge, UK), cardiac troponin I (cTnI) (ab246529; Abcam), and myoglobin (MB) (ab260068; Abcam) in myocardial tissues and cardiomyocytes with H/R were measured using corresponding enzyme-linked immunosorbent assay kits by following the protocols of the manufacturers.
3
Cardiac Enzymes and Cytokine Profiling
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ELISA kits were consumed to measure cardiac enzymes; CPK (ab187396), CK-MB (ab187396), cTnT (ab246529) and cTnI (ab246529) (all obtained from Abcam, Eugene, OR, USA), following the manufacturer’s protocols [45 (link)]. The concentration of serum cytokines TNF-α, IL-17, IL-10, and IL-6 was determined using ELISA kits (R&D Systems; Minneapolis, MN, USA). Serum IL-2 and IL-4 were determined by assay kits (Eagle Biosciences, Inc., Amherst, NH, USA).
4
Hormonal Biomarkers in Plasma and Serum
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