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Trimethyl h3 k27

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Trimethyl-H3-K27 is a modified histone H3 protein that is trimethylated at the lysine 27 residue. It is a commonly used marker in epigenetic research to study chromatin organization and gene regulation.

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Lab products found in correlation

Sodium salt of isobutyric acid, by Merck Group (2 mentions) Isovaleric acid, by Merck Group (2 mentions) Sodium propionate, by Merck Group (2 mentions) Cd19 pe, by BioLegend (2 mentions) Antibodies to acetyl h3 k9, by ABclonal (2 mentions) Acetyl h3 k18, by ABclonal (2 mentions) Trimethyl h3 k9, by Beyotime (2 mentions) Easysep human cd3 selection cocktail 2, by STEMCELL (2 mentions) Human cd19 selection cocktail 2, by STEMCELL (2 mentions) Cd2 apc, by BioLegend (2 mentions) Ecl reagent kit, by Beyotime (2 mentions) Chemiscope 6000 touch imaging system, by Clinx (2 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Merck Group (2 mentions) Primescript rt master mix, by Takara Bio (2 mentions) Sodium acetate, by Merck Group (2 mentions) Sodium butyrate, by Merck Group (2 mentions) Gapdh, by Abcam (2 mentions) Sybr green pcr master mix, by Vazyme (2 mentions)

2 protocols using trimethyl h3 k27

1

Epigenetic Regulation Experimental Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium acetate (catalog no. S2889), sodium butyrate (catalog no. 303410), sodium propionate (catalog no. P5436), sodium salt of isobutyric acid (catalog no. I1754), and isovaleric acid (catalog no. 129542) were purchased from Sigma-Aldrich. The compounds were used in the form of sodium salt. Antibodies to acetyl-H3-K9 (Abclonal; catalog no. A7255), acetyl-H3-K18 (Abclonal; catalog no. A7257), trimethyl-H3-K9 (Beyotime; catalog no. AF5707), trimethyl-H3-K27 (Beyotime; catalog no. AF5710), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (Abcam; catalog no. AB8245) were obtained commercially. PrimeScript RT master mix (TaKaRa; catalog no. RR047A) and SYBR green PCR master mix (catalog no. Q141-02/03; Vazyme, Nanjing, China) were obtained commercially. Lymphocyte separation reagent was purchased from Tianjin Haoyang TBD (catalog no. LTS1077). EasySep human CD3+ selection cocktail II (Stem Cell; catalog no. 17851), human CD19+ selection cocktail II (Stem Cell; catalog no. 17854), CD2-APC (BioLegend; catalog no. 300311), and CD19-PE (BioLegend; catalog no. 302207) were purchased from commercial sources. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Sigma-Aldrich. Immunoblots were detected using an ECL reagent kit (Beyotime) and the ChemiScope 6000 Touch imaging system (Clinx, China).
Wang L., Xu D., Huang Q., Yang G., Zhang M., Bi J., Shan J., Li E, & He S. (2021). Characterization of Tonsil Microbiota and Their Effect on Adenovirus Reactivation in Tonsillectomy Samples. Microbiology Spectrum, 9(2), e01246-21.
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2

Epigenetic Regulation Experimental Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium acetate (catalog no. S2889), sodium butyrate (catalog no. 303410), sodium propionate (catalog no. P5436), sodium salt of isobutyric acid (catalog no. I1754), and isovaleric acid (catalog no. 129542) were purchased from Sigma-Aldrich. The compounds were used in the form of sodium salt. Antibodies to acetyl-H3-K9 (Abclonal; catalog no. A7255), acetyl-H3-K18 (Abclonal; catalog no. A7257), trimethyl-H3-K9 (Beyotime; catalog no. AF5707), trimethyl-H3-K27 (Beyotime; catalog no. AF5710), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (Abcam; catalog no. AB8245) were obtained commercially. PrimeScript RT master mix (TaKaRa; catalog no. RR047A) and SYBR green PCR master mix (catalog no. Q141-02/03; Vazyme, Nanjing, China) were obtained commercially. Lymphocyte separation reagent was purchased from Tianjin Haoyang TBD (catalog no. LTS1077). EasySep human CD3+ selection cocktail II (Stem Cell; catalog no. 17851), human CD19+ selection cocktail II (Stem Cell; catalog no. 17854), CD2-APC (BioLegend; catalog no. 300311), and CD19-PE (BioLegend; catalog no. 302207) were purchased from commercial sources. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Sigma-Aldrich. Immunoblots were detected using an ECL reagent kit (Beyotime) and the ChemiScope 6000 Touch imaging system (Clinx, China).
Wang L., Xu D., Huang Q., Yang G., Zhang M., Bi J., Shan J., Li E, & He S. (2021). Characterization of Tonsil Microbiota and Their Effect on Adenovirus Reactivation in Tonsillectomy Samples. Microbiology Spectrum, 9(2), e01246-21.
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