Mcf 7 cell

A549 cells were cultured in Minimum Essential Medium Eagle (MEM) with L-glutamine and sodium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA) in the presence of 10% fetal calf serum, 1 mM pyruvate, 10 mM HEPES, 50 μg/mL penicillin, and 50 μg/mL streptomycin (all supplements from Life Technologies, Grand Island, NY, USA). MCF-7 cells were maintained in MEM alpha modification with L-glutamine and sodium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal calf serum, 1 μg/mL insulin, 50 μg/mL penicillin, and 50 μg/mL streptomycin (all reagents from Life Technologies, Grand Island, NY, USA). HepG2 were propagated in Dulbecco’s Modified Eagle’s Medium DMEM—a high glucose medium (Sigma-Aldrich, St. Louis, MO, USA)—supplemented with 10% (v/v) fetal bovine serum, 2 mM L-glutamine, 1 mM pyruvate, 10 mM HEPES, 10 µL/mL MEM non-essential amino acids, 50 µg/mL penicillin, and 50 µg/mL streptomycin (all reagents from Life Technologies, Grand Island, NY, USA). A549, MCF-7, and HepG2 cells were purchased from the European Collection of Cell Cultures (ECACC, Salisbury, UK). The cell cultures were maintained under standard cell culture conditions at 37 °C in a humidified incubator under an atmosphere of 5% CO₂ and 95% air. Cells were passaged every 2–3 days to obtain exponential growth. The cells were maintained in the culture for no more than 20 passages.

MCF-7 and MDAMB-231 cells were obtained from American Type Culture Collection (ATCC, USA). MCF-7 cells were maintained in DMEM and MDAMB-231 cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) 100 U/ml, Penicillin and 100 mg/ml streptomycin sulfate (Sigma) at 37 °C with 5% CO2 in a 95% humidified atmosphere.

MCF-7 cells were purchased from the American Type Culture Collection (ATCC) and MFM223 cells from Sigma-Aldrich. MDA-MB-231(SA) cells were a gift from Dr. Guise (University of Texas, USA). MDA-MB-231(SA) is a variant of MDA-MB-231 that effectively forms bone metastases, but does not otherwise markedly differ from the parental cell line [22 (link), 23 (link)]. MCF-7 cells were grown in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (iFBS, Gibco), GlutaMAX (Gibco), estrogen (10^–9 M) and insulin (4 μg/ml). MDA-MB-231(SA) cells were grown in 10% iFBS/DMEM (Sigma-Aldrich) supplemented with GlutaMAX and non-essential amino acids (Gibco). MFM223 cells were grown in 10% iFBS/DMEM. The FGFRis TKI258 (S2769), BGJ398 (S2183) and AZD4547 (S2801) were purchased from Selleck Chemicals as DMSO stocks (IC50 values are listed in Supplementary Table S1).

T-47D and MCF-7 cells were purchased from American Type Culture Collection (ATCC). T-47D cells were cultured in RPMI medium (Sigma-Aldrich) and MCF-7 cells were cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Genesee Scientific) and 2% Penicillin–Streptomycin (Sigma-Aldrich) in a humidified incubator with 5% CO₂. Generation of ESR1 mutant (Y537S and D538G) MCF-7 and T-47D cells were previously described^{8 (link),23 (link)}. Tamoxifen-resistant MCF-7 cells were provided by Dr. Carlos Arteaga. Cells were assessed for their viability and counted with a Countess II FL Automated Cell Counter (Life Technologies, Gaithersburg, MD). The cells were regularly tested for Mycoplasma contamination with PlasmoTest (Invivogen).

MCF-7 cells were purchased from ATCC (American Type Culture Collection), (Manassas, VA, USA). MCF-7 cells were cultured in DMEM (Dulbecco’s modified Eagle’s medium) (Sigma) and complemented with fetal bovine serum (FBS10%) (Sigma St. Louis, MO), 100 I.U/mL penicillin, and 100 μg/mL streptomycin (Sigma St. Louis, MO). The cells were cultivated at 37 °C and 5% CO₂ in a humidified incubator.