Specific binding to the receptors was defined as the difference between total binding and non-specific binding, as determined in the presence of an excess of unlabelled ligand. Binding isotherm curves were analysed to determine the equilibrium binding parameters, affinity (dissociation constant [Kd]) and maximal binding capacity of labelled ligand. Competition curves were analysed to assess the half-maximal inhibitory concentration (IC50). The affinity of test compounds (inhibition constant [Ki]) was then calculated according to the Cheng-Prusoff equation: Ki = IC50/(1 + [C]/Kd). Data were analysed by non-linear curve fitting using Prism for Windows version 6 software (GraphPad Software, La Jolla, CA, USA). The results of replicated curves were expressed as mean ± SEM.
Prism version 6 for windows
Manufactured by GraphPad
Sourced in United States
GraphPad Prism Version 6 for Windows is a data analysis and graphing software. It provides tools for data management, statistical analysis, and visualization. The software is designed to help researchers and scientists analyze and present their data effectively.
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Lab products found in correlation
13 protocols using prism version 6 for windows
1
Ligand Binding Assay Analysis
2
Statistical Analysis of Biological Data
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For statistics (unpaired t test with two-tailed p values, Mann-Whitney u test, column statistics) we used Prism for Windows version 6 software (GraphPad Software, La Jolla, CA, USA). p Values ≤0.05 were considered significant.
3
Analyzing Surgical Outcomes in Orthognathic Surgery
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The statistical analysis was performed using GraphPad Prism for Windows, version 6.0 (GraphPad Software, Inc., La Jolla, CA, USA). Holm-Sidak’s multiple comparisons test and two-way ANOVA test with a post hoc Sidak correction method were performed to determine significant differences in OCC and TV before and after orthognathic surgery. The OCC and TV reductions after orthognathic surgery were measured using linear regression analysis. The TV/OCC ratios from T0 to T3 were analyzed using one-way ANOVA and Geisser-Greenhouse correction. The results were considered significant at P < 0.001.
4
Statistical Analysis of Experimental Data
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These analyses were performed with the software GraphPad Prism for Windows, version 6.0 (GraphPad Software, Inc., La Jolla, CA, EUA). The parametric quantitative variables were compared through a T-test or One-way ANOVA with multiple comparisons through the Bonferroni test. The non-parametric quantitative variables were compared through the Mann-Whitney or by Kruskal-Wallis tests with multiple comparisons through the Dunn's test. A p-value (p < 0.05) represented a significant difference among two groups.
5
Statistical Analysis for Group Comparisons
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Means were compared across groups using one-way analysis of variance (ANOVA) for ≥ three groups or t-tests for two groups for data with normal distribution that met variance homogeneity. Significance was assessed using the Tukey criterion for pairwise mean comparisons under the ANOVA model. Normality was assessed using the Kolmogorov-Smirnov test. When homogeneity of variance was not met under the ANOVA model, a robust ANOVA was carried out. Two-tailed p-values <0.05 were considered significant. Values are expressed as mean ± SE. Computations were carried out using Graphpad Prism for Windows, version 6.0 (La Jolla, CA).
6
Statistical analysis of sheep data
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Statistical analysis was performed using GraphPad Prism for Windows Version 6.0 (GraphPad Software Inc, La Jolla, USA. Each parameter was assessed for Gaussian distribution both visually and using the D’Agostino & Pearson omnibus normality test. Comparisons between individual lobes within the same sheep and comparisons of pre- and post- treatment were performed using paired analyses. For normally distributed data, paired two-tailed t test were used. Wilcoxon matched-pairs signed rank test was used for data that did not meet assumptions required for parametric testing. To assess for associations between two measured parameters, Correlations were assessed by the Spearman correlation coefficient (rs) A P value of <0.05 was taken as significant. Values are reported as mean ± SEM, unless stated.
7
Comparative Sheep Lobe Analysis
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Statistical analysis was performed using GraphPad Prism for Windows Version 6.0 (GraphPad Software Inc., La Jolla, CA). Each parameter was assessed for Gaussian distribution both visually and using the D'Agostino and Pearson omnibus normality test. Comparisons between individual lobes within the same sheep and comparisons of before and after treatment were performed using paired analyses. For normally distributed data, paired two-tailed t tests were used. Wilcoxon matched-pairs signed rank test was used for data that did not meet assumptions required for parametric testing. Differences between the Senciapoctreated sheep and control group (between lobes of different animals) were assessed using a Student's t test for data with a normal distribution and a Mann-Whitney test for data with a nonparametric distribution. A P value less than 0.05 was taken as significant. Values are reported as mean (6SEM), unless otherwise stated.
8
Statistical Analysis of Experimental Data
9
Purification and Characterization of RelA
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DTT, BSA and antibiotics were purchased from Melford Laboratories; polyacrylamide-bis polyacrylamide [30% (w/v), 37:5:1], Bacto tryptone and yeast extract for culture media were purchased from Oxoid. Chelating fast flow resin and Superdex 200 resin were purchased from GE Healthcare; primers were purchased from Eurofins; restriction enzymes and E. coli strain K12 JM109 were purchased from New England Biolabs; pET16b plasmid was purchased from Merck Chemicals; E. coli RelA was expressed using a strain from the ASKA Clone library purchased from Shigen; E. coli MRE600 (C6) strain was purchased from NCTC. Francisella philomiragia was obtained from A.T.C.C.; mRNA was purchased from ATDBio. Unless stated otherwise all other reagents were purchased from Sigma–Aldrich or Fisher Scientific. Graphpad Prism version 6 for Windows was obtained from Graphpad Software.
10
Detailed Statistical Analysis Techniques for Nociceptive Behavior
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Two-way analysis of variance (ANOVA) with repeated measures followed by Bonferroni
post-hoc test was used to analyze the time course of mechanical hyperalgesia, according to
condition (i.e., sham/CCI-ION) and time as independent factors. Two-way ANOVA followed by
Bonferroni post-hoc test was performed to analyze the USV emission after d,l-amphetamine
treatment. Analyzes of western blot and immunofluorescence intensity were performed using
the unpaired two-tailed Student’s t-test. Sample size was determined based on the GPower
3.1 software31 (link)
defining a large standardized effect size of F = 0.5; power of 0.8; and α = 0.05,
and estimated six to eight rats per group in the different experimental designs. For c-Fos
detection, the sample size was based on previous studies32 (link),33 (link) to recruit the smallest number of rats.
All data were expressed as mean ± SEM, and the results were considered statistically
significant if p < 0.05. GraphPad Prism version 6 for Windows was used
for statistical analysis of data (GraphPad Software, San Diego, CA).
post-hoc test was used to analyze the time course of mechanical hyperalgesia, according to
condition (i.e., sham/CCI-ION) and time as independent factors. Two-way ANOVA followed by
Bonferroni post-hoc test was performed to analyze the USV emission after d,l-amphetamine
treatment. Analyzes of western blot and immunofluorescence intensity were performed using
the unpaired two-tailed Student’s t-test. Sample size was determined based on the GPower
3.1 software31 (link)
defining a large standardized effect size of F = 0.5; power of 0.8; and α = 0.05,
and estimated six to eight rats per group in the different experimental designs. For c-Fos
detection, the sample size was based on previous studies32 (link),33 (link) to recruit the smallest number of rats.
All data were expressed as mean ± SEM, and the results were considered statistically
significant if p < 0.05. GraphPad Prism version 6 for Windows was used
for statistical analysis of data (GraphPad Software, San Diego, CA).
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