Standard samples

Manufactured by Merck Group

Standard samples are reference materials used to calibrate and verify the performance of laboratory equipment and analytical methods. They provide a consistent and reliable basis for measurements, ensuring the accuracy and consistency of test results.

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Lab products found in correlation

Agilent 1200, by Agilent Technologies (1 mentions)

2 protocols using standard samples

Quantification of Blood Thiamine Metabolites

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Thiamine, TMP, and TDP levels in whole blood samples were measured using HPLC, based on the established method [4 ] with slight modification. Briefly, blood samples were collected using heparin-anticoagulated tubes; 150 ul sample was vibrated for 30 s with equal volume of 5.2% perchloric acid (PCA) added dropwise for deproteinization. Then, the mixture was stored at − 80 °C until assay within 1 month. The mixture was centrifuged at 12,000 rpm for 8 min at 4 °C; the supernatant was pipetted. Thiamine, TMP, and TDP in supernatant were derivatized into thiochromes using potassium ferricyanide and analyzed by gradient elusion with C₁₈ reversed-phase analytical column (250 * 4.6 mm). The derivatives were identified by HPLC fluoroscopy (1100, Agilent Technologies Inc., ex: 367 nm, em: 435 nm). The thiamine, TMP, and TDP levels were quantified using standard samples (Sigma-Aldrich, St. Louis, MO). The analyzers were blinded to samples information.

Wang C., Zhang S., Zou Y., Ma H., Jiang D., Sheng L., Sang S., Jin L., Guan Y., Gui Y., Xu Z, & Zhong C. (2020). A novel PET tracer 18F-deoxy-thiamine: synthesis, metabolic kinetics, and evaluation on cerebral thiamine metabolism status. EJNMMI Research, 10, 126.

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Quantitative Analysis of Catechins by HPLC

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The content and composition of catechins were studied on an analytical HPLC system consisting of a chromatograph Agilent 1200 with a diode array detector and a ChemStation system according to the protocol described in our previous study (Zheleznichenko et al. 2018 (link)). The individual compound quantitative estimations in the eluates were quantified according to an external standard method (Van Beek 2002 (link)). Standard samples (Sigma-Aldrich) were prepared at 10.0 μg mL⁻¹ concentration in ethyl alcohol. UF spectra of unidentified components were specific for catechins: the spectral range was 246 to 325 nm with λ max 270 to 280 nm. The amount of catechins was expressed as milligram of ± catechin equivalents per gram dry weight of the sample (mg CE g⁻¹).

Zheleznichenko T., Voronkova M., Asbaganov S., Kukushkina T., Filippova E., Protsenko M., Mazurkova N, & Novikova T. (2023). Impact of different Agrobacterium rhizogenes strains on secondary metabolites accumulation in Nitraria schoberi L. hairy roots and antiviral activity of their extracts against influenza virus of subtypes A (H5N1) and A (H3N2). In Vitro Cellular & Developmental Biology.

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