Microfuge 16 centrifuge

Manufactured by Beckman Coulter

Sourced in United States, Germany

The Microfuge® 16 Centrifuge is a compact, benchtop centrifuge designed for a variety of laboratory applications. It features a maximum speed of 16,000 rpm and a maximum relative centrifugal force (RCF) of 21,130 x g. The Microfuge® 16 Centrifuge accommodates a range of sample tube sizes and can be used for various sample preparation and separation procedures.

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Lab products found in correlation

Nanodrop 2000, by Thermo Fisher Scientific (2 mentions) Genelute bacterial genome dna kit, by Merck Group (1 mentions) Eps 301, by GE Healthcare (1 mentions) Cfx96 real time pcr system, by Bio-Rad (1 mentions) Flat bottom 96 well plate, by Greiner (1 mentions) Geno grinder, by SPEX (1 mentions) Thermoshaker, by Avantor (1 mentions)

Close spelling variants of this product

Microfuge 16 (21 citations) Microfuge (10 citations)

6 protocols using microfuge 16 centrifuge

Genome Sequencing of Mutant Lactococcus Strains

Cited 1 time

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The mutated IPLA838 cells (MUTproducers) selected by FACS were isolated on M17-agar plates. A single colony of each strain was grown as standing culture in 5 mL of M17 broth, supplemented with 0.5% (w/v) lactose, and incubated overnight at 30 °C. Cells from the three cultures were collected by centrifugation at 10,000 rpm for 3 min in a Microfuge 16 centrifuge (Beckman Coulter, Woerden, The Netherlands). Genomic DNA was isolated with a GenElute bacterial genome DNA kit (Sigma-Aldrich, Munich, Germany) according to the manufacturer’s instructions.
The genomes of all different colonies were paired-end sequenced at the Beijing Genomics Institute (BGI, Copenhagen N, Denmark) on a BGISEQ-500 platform. A total of 5 million paired-end reads (150 bp) were generated. FastQC version 0.11.589 was used to examine the quality of the reads. Identification of mutations was performed with Breseq (Deatherage and Barrick, 2014 (link)), using the complete genome of Lactococcus lactis subsp. lactis IPLA838, as a reference sequence.

Hernandez-Valdes J.A., aan de Stegge M., Hermans J., Teunis J., van Tatenhove-Pel R.J., Teusink B., Bachmann H, & Kuipers O.P. (2020). Enhancement of amino acid production and secretion by Lactococcus lactis using a droplet-based biosensing and selection system. Metabolic Engineering Communications, 11, e00133.

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Determining Optimal Bacteriophage Infection MOI

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To determine the optimal MOI, the bacterial culture was infected with the bacteriophages at 5 different MOI ratios: 0.001, 0.01, 0.1, 1 and 10. The infected cells were incubated for 6 h at 37 °C with mild agitation (170 rpm) after incubation; the suspension was centrifuged for 10 min at 10,000 rpm using Beckman Coulter^® Microfuge^® 16 Centrifuge to obtain clear supernatants for the bacteriophage titer assay. The MOI with the highest bacteriophage titer was taken as the optimal MOI. All the experiments were performed in triplicate and the results were taken as the average of the three replicates [22 (link)].

Baqer A.A., Fang K., Mohd-Assaad N., Adnan S.N, & Md Nor N.S. (2022). In Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-Resistant Klebsiella pneumoniae. Viruses, 15(1), 117.

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Thermophilic Bacterium Cultivation Protocol

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A. kamchatkensis NASTPD13 was cultured on nutrient agar at 60°C for 18 h. Single colony was transferred into the nutrient broth and incubated at 60°C at 200 rpm for 18 h. Cells were harvested by centrifugation at 10,000 × g for 5 min using a Microfuge® 16 centrifuge (Beckman Coulter, Brea, CA, USA).

Yadav P., Sharma S., Bhattarai T., Sreerama L., Prasad G.S., Sahni G, & Maharjan J. (2021). Whole-Genome Sequence Data Analysis of Anoxybacillus kamchatkensis NASTPD13 Isolated from Hot Spring of Myagdi, Nepal. BioMed Research International, 2021, 1869748.

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Molecular Techniques for Biomedical Research

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KS12 biological safety cabinet (Thermo Fisher Scientific, Dreieich, Germany); Lab Dancer (IKA, Königswinter, Germany); Microfuge^® 16 centrifuge (BECKMAN, Brea, CA, USA); DK-S26 electric thermostatic water bath (Shanghai Senxin Experimental Instrument Co., Ltd., Shanghai, China); Nanodrop 2000 (ThermoFisher Scientific, Germany); Biometra Tadvanced 96 SG (Biometra, Göttingen, Germany); Lab cycler Gradient (SensoQuest, Göttingen, Germany); CFX96 Real-Time PCR system (Bio-RAD, Hercules, CA, USA); Automatic Gel Imaging analysis SystemZF-258 (Shanghai Jiapeng Technology Co., Ltd., Shanghai, China); Electrophoresis power supply EPS 301 (GE, Boston, MA, USA).

Ding L., Xu X., Wang X., Chen X., Lu Y., Xu J, & Peng C. (2023). Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods. Foods, 12(19), 3681.

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Chlorophyll Quantification in Leaf Samples

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Freeze-dried leaf samples were ground to fine powder using a ball-mill grinder (2010 Geno/Grinder^®, SPEX SamplePrep). Chlorophylls were extracted in cold 100% methanol by shaking for 3 h; samples were then centrifuged for 15 min at 1500 rpm (Microfuge^® 16 Centrifuge, Beckman Coulter^®, Indianapolis, IN, USA) and supernatants were collected, all at 4 °C. Measurements were taken on 200 µl of the sample (or blank) in a 96-well flat-bottom plate (Greiner Bio-One, Frickenhausen, Germany) using a microplate reader (Multiscan^® Spectrum Microplate Spectrophotometer, Thermo Electron Corporation, Vantaa, Finland) at wavelengths of 470, 652.4, and 665 nm. Chl a and b concentrations (Supplementary Fig. S9) were calculated using the equations as described by Wellburn (1994) , with modifications to the equations for the pathlength of the microplate reader (with 200 µl) following the method of Warren (2008) .

Kotula L., Clode P.L., Jimenez J.D, & Colmer T.D. (2019). Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells. Journal of Experimental Botany, 70(18), 4991-5002.

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Optimized Extraction and Analysis of Dye Mixtures from Polyester Fibers

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For optimization and validation of the developed method, methanol stock solutions of commercially available dyeing mixtures (D1–D9, Table 6) at a concentration of 1 mg·mL⁻¹ were used as standards. In total, 10 μL of each of the stock solutions were placed in a PCR tube, then the solvent was evaporated under a nitrogen atmosphere at 40 °C using a sample concentrator (Liebisch, Germany). Subsequently, the residues were dissolved in 50 µL of BGE, centrifuged for 5 min at 12,000× g rpm (Microfuge 16 Centrifuge, Beckman Coulter, Krefeld, Germany), and analyzed.
Polyester threads of 1 or 4 cm length (consisting of approximately 50 single fibers) were used as a sample. Extraction was carried out in glass vials closed with caps with 50 μL of chlorobenzene at 100 °C in a thermoshaker (VWR, Radnor, PA, USA) operating at 1000 rpm for 0.5, 1, or 6 h, depending on the experiment. The preparation of five samples (P10, P13a, P13b, P17, P18) required some adjustments (see Table 8 for details).
The extracts were transferred to PCR tubes with the use of a micro syringe to avoid transferring microfibers. The extracts were then evaporated to dryness under gentle nitrogen flow (40 °C) and dissolved similarly to dye standards.

Sałdan A., Król M., Śmigiel-Kamińska D., Woźniakiewicz M, & Kościelniak P. (2022). Development of the Microemulsion Electrokinetic Capillary Chromatography Method for the Analysis of Disperse Dyes Extracted from Polyester Fibers. Molecules, 27(20), 6974.

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