Vega3 lmu scanning electron microscope

Manufactured by TESCAN

Sourced in Czechia

The Vega3 LMU scanning electron microscope is a powerful imaging tool designed for high-resolution analysis of a wide range of materials. It features a large specimen chamber, flexible sample manipulation, and advanced imaging capabilities to provide detailed information about the surface structure and composition of samples.

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Lab products found in correlation

Conductive silver paint, by Agar Scientific (3 mentions) Em cpd300, by Leica (3 mentions) S150b sputter coater, by TESCAN (2 mentions) K550x sputter coater, by Emitech (1 mentions) Camedia c 5060, by Olympus (1 mentions) Bx41 light microscope, by Olympus (1 mentions)

12 protocols using vega3 lmu scanning electron microscope

Scanning Electron Microscopy Analysis of HAP Nanoparticles

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The survey was carried out using a TESCAN Vega 3 LMU scanning electron microscope (TESCAN, Brno–Kohoutovice, Czech Republic) with a tungsten cathode. The HAP NPs dispersion was treated with ultrasound with a power of 65 W for 30 min for destruction of the possibly formed agglomerates. A drop of dispersion was applied to an electrically conductive graphite tape with an automatic pipette with disposable tips, and it was then dried in vacuum without heating. The survey was carried out at a 10 kV accelerating voltage and 4 mm working distance.
The composites were studied using a TESCAN Vega 3 LMU scanning electron microscope with a tungsten cathode. A thin layer of the sample was placed on a graphite substrate. The survey was carried out at an accelerating voltage of 5 kV, and a working distance of 3.1 mm.
The size distribution of HAP NPs was determined based on statistical data.
For each concentration of surfactants used in the synthesis, the size of at least 100 formed particles was estimated. Histograms of the particle size quantitative distribution were constructed based on the data obtained. In the case of lamellar morphology particles, the length and thickness of the particles were evaluated; in the case of rod-shaped particles, the length and diameter were evaluated.

Gordienko M., Karakatenko E., Menshutina N., Koroleva M., Gilmutdinova I, & Eremin P. (2021). Composites Composed of Hydrophilic and Hydrophobic Polymers, and Hydroxyapatite Nanoparticles: Synthesis, Characterization, and Study of Their Biocompatible Properties. Journal of Functional Biomaterials, 12(4), 55.

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Scanning Electron Microscopy of C. albicans

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The C. albicans cells were incubated at 37 °C for 30 min without (control) or with AmB at the concentration of 20 µM. Next, the cells were fixed with 4% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) for 2 h at a low temperature (4 °C). Then, the samples were carefully washed with 0.1 M phosphate buffer (pH 7.2). Post-fixation was carried out for 2 h, with 1% osmium tetroxide at 4 °C. Then the cells were rinsed with 0.1 M phosphate buffer (pH 7.2). After washing, dehydration in the series of ethanol gradients: 30%, 50%, 70%, 90%, and 100% (each for 10 min), was performed. Subsequently, the specimens were chemically dried with application of 98% hexamethyldisilazane (HMDS). Finally, the specimens were coated with gold in Emitech K550X Sputter Coater. The samples were imaged with TESCAN vega 3 LMU scanning electron microscope (Czech Republic).

Grela E., Zdybicka-Barabas A., Pawlikowska-Pawlega B., Cytrynska M., Wlodarczyk M., Grudzinski W., Luchowski R, & Gruszecki W.I. (2019). Modes of the antibiotic activity of amphotericin B against Candida albicans. Scientific Reports, 9, 17029.

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Particle Characterization Using Microscopy

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The particles were examined with the use of an Olympus BX41 light microscope (Olympus, Hamburg, Germany). Images were registered with a photographic camera (Olympus Camedia C-5060, Hamburg, Germany). Images obtained at 40× magnification were binarized and segmented (Fiji image processing). ImageJ software was used to measure particle size parameters.¹² Feret diameter and circularity were selected as the most representative shape descriptors. The results were analyzed in GrapPad Prism 5.0 (GrapPad Software, San Diego, CA). The surface layer of the samples was observed with a TESCAN VEGA3 LMU scanning electron microscope (SEM) (Tescan, Brno, Czech Republic). A graphite double-sided adhesive tape was covered with the sample; afterwards, the sample was dehydrated and sputter-coated with gold (Emitech K550X, Quorum Technologies, UK). The analyses were performed at acceleration voltage of 30 kV.

Danielewicz A., Wójciak M., Sawicki J., Dresler S., Sowa I, & Latalski M. (2020). Comparison of Different Surgical Systems for Treatment of Early-onset Scoliosis in the Context of Release of Titanium Ions. Spine, 46(10), E594-E601.

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Ultrastructural Analysis of Candida albicans

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C. albicans cells
were incubated at 37 °C for 1 and 2 h with AmB, AmBisome, Fungizone,
or with AmB–BSA complex at the AmB concentration of 2 μM.
The control cells were incubated with PBS, 1% DMSO, or BSA. After
gentle centrifuging (2500g, at 4 °C), the pellet
of the cells was fixed with 4% glutaraldehyde in 0.1 M phosphate buffer
pH 7.2 for 2 h at 4 °C. Then, the samples were rinsed with 0.1
M phosphate buffer (pH 7.2). After washing, post-fixation was performed
with freshly prepared 1% osmium tetroxide at 4 °C for 2 h. The
subsequent rinsing was carried out with the usage of 0.1 M phosphate
buffer (pH 7.2). Then, the cells were dehydrated in a series of ethanol
gradients: 30, 50, 70, 90, and 100% (each for 10 min). The next step
was chemical drying with the application of 98% hexamethyldisilazane
(HMDS). Eventually, the specimens were coated with gold in Emitech
K550X Sputter Coater. The sample analysis was performed with a TESCAN
vega 3 LMU scanning electron microscope (Czech Republic). For every
experimental variant, the percentage of altered cells was calculated
from at least eight microscopic fields of observed cells. Then, the
percentage was averaged.

Grela E., Stączek S., Nowak M., Pawlikowska-Pawlega B., Zdybicka-Barabas A., Janik S., Cytryńska M., Grudzinski W., Gruszecki W.I, & Luchowski R. (2023). Enhanced Antifungal Activity of Amphotericin B Bound to Albumin: A “Trojan Horse” Effect of the Protein. The Journal of Physical Chemistry. B, 127(16), 3632-3640.

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Ultrastructural Analysis of C. albicans Treated with X33 AMOP

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C. albicans cells were incubated with X33 AMOP (MIC) at 37°C for 0, and 4 h (Ma et al., 2020 (link)). Thereafter, the cells were fixed with 2.5% glutaraldehyde and dehydrated with 50%, 60%, 70%, 80%, 90%, 95% and 100% ethanol gradients, followed by drying using 98% hexamethyldisilazane (HMDS). The samples were then coated with gold and imaged with the TESCAN vega 3 LMU scanning electron microscope (SEM).
Since the changes in internal cellular morphology can be used to evaluate the antifungal effect of X33 AMOP on C. albicans, the cells were incubated with X33 AMOP (MIC) in the YPD medium at 37°C for 0, 4, and 12 h (Su et al., 2020 (link)). The cells were then fixed with 2.5% glutaraldehyde and 1% osmic acid solution at 4°C. After dehydrating with 50%, 60%, 70%, 80%, 90%, 95% and 100% ethanol, the samples were treated with an embedding agent and acetone. The samples were then sliced in an ultra-thin microtome and stained with lead citrate solution, followed by a saturated solution of uranyl acetate and 50% for 10 min each. Thereafter, the stained samples were observed by transmission electron microscope (TEM).

Lu Q., Wang Y., Liao X., Zhou F., Zhang B, & Wu X. (2023). Physiological and transcriptome analysis of Candida albicans in response to X33 antimicrobial oligopeptide treatment. Frontiers in Cellular and Infection Microbiology, 13, 1123393.

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Morphological Analysis of Black Locust Flowers

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The morphology of black locust flowers before and after the UAE, maceration, and Soxhlet extraction was analyzed using the Vega-3 LMU Scanning Electron Microscope (Tescan, Brno, Czech Republic) under high vacuum conditions. The SEM was used at an accelerating voltage at 20 kV and a magnification of 2000 × (10 µm). The plant material was mounted on a metal grid with double-sided adhesive tape, and then the bead surfaces were coated with chrome under vacuum.

Savic Gajic I., Savic I., Boskov I., Žerajić S., Markovic I, & Gajic D. (2019). Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods. Antioxidants, 8(8), 248.

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Scanning Electron Microscopy of Mouse Cochlea

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For SEM, the dissected mouse cochleae were initially fixed by a very gentle intralabyrinthine perfusion using a 10 μL pipette tip through the round window. The fixative contained 2.5% v/v glutaraldehyde in 0.1 m sodium cacodylate buffer plus 2 mm Ca₂Cl (pH 7.4). After a few minutes, the cochleae were immersed in the above fixative for 2 h at room temperature. After the fixation, the organ of Corti was exposed by removing the bone from the apical coil to the cochlea and then immersed in 1% osmium tetroxide in the cacodylate buffer for 1 h. For osmium impregnation, which avoids gold coating, cochleae were incubated in solutions of saturated aqueous thiocarbohydrazide (20 min) alternating with 1% osmium tetroxide in buffer (2 h) twice (the OTOTO technique: Furness & Hackney, 1986). The cochleae were then dehydrated through an ethanol series and critical point dried using CO₂ as the transitional fluid (EM CPD300; Leica) and mounted on specimen stubs using conductive silver paint (Agar Scientific, Stansted, UK). The apical coil of the organ of Corti was examined at 10 kV using a Vega3 LMU scanning electron microscope (Tescan, Brno, Czechia).

Carlton A.J., Halford J., Underhill A., Jeng J., Avenarius M.R., Gilbert M.L., Ceriani F., Ebisine K., Brown S.D., Bowl M.R., Barr‐Gillespie P.G, & Marcotti W. (2020). Loss of Baiap2l2 destabilizes the transducing stereocilia of cochlear hair cells and leads to deafness. The Journal of Physiology, 599(4), 1173-1198.

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Scanning Electron Microscopy of ift88 Zebrafish Larvae

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For scanning electron microscopy, ift88 homozygous mutant and phenotypically wild-type sibling larvae at 4 dpf were fixed overnight in 2.5% glutaraldehyde/0.1M sodium cacodylate buffer. Samples were washed in buffer, post-fixed in 2% aqueous osmium tetroxide for 1 h, washed in buffer again and then dehydrated through a graded ethanol series (50, 75, 95, 100%) before being dried in a mixture of 50% hexamethyldisilazane (HMDS) in 100% ethanol. Final drying was in 100% HMDS. After removal of the final HMDS wash, samples were left to dry in a fume hood overnight. Samples were mounted onto a pin stub using a Leit-C sticky tab and Leit-C mounting putty, gold-coated using an Edwards S150B sputter coater, and examined in a Tescan Vega3 LMU Scanning Electron Microscope at an operating voltage of 15 kV and imaged using a secondary electron detector.

Cheung K.Y., Jesuthasan S.J., Baxendale S., van Hateren N.J., Marzo M., Hill C.J, & Whitfield T.T. (2021). Olfactory Rod Cells: A Rare Cell Type in the Larval Zebrafish Olfactory Epithelium With a Large Actin-Rich Apical Projection. Frontiers in Physiology, 12, 626080.

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Scanning Electron Microscopy of Insect Antennae

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From P. corruscus collected from Middlebury, VT in May–June, 2021, antennae were removed while under CO₂ anesthesia and then dried sequentially with hexane, acetone, and 95% ethanol in a watch glass. Dried antennae were mounted on an aluminum specimen mount and coated with gold-palladium using an EffaCoater Au-Pd Sputter Coater (Ernest Fullam Inc.; Latham NY, USA). Coated antennae were then imaged using a Vega 3 LMU Scanning Electron Microscope (Tescan; Brno, Czech Republic) with an accelerating voltage of 5 mV and a beam intensity of 8.

Lower S.E., Pask G.M., Arriola K., Halloran S., Holmes H., Halley D.C., Zheng Y., Collins D.B, & Millar J.G. (2023). Identification of a Female-Produced Sex Attractant Pheromone of the Winter Firefly, Photinus corruscus Linnaeus (Coleoptera: Lampyridae). Journal of Chemical Ecology, 49(3-4), 164-178.

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Scanning Electron Microscopy of Mouse Cochlea

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For SEM, the dissected mouse cochleae were initially fixed by a very gentle intralabyrinthine perfusion using a 100-μL pipette tip through the round window. The fixative contained 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer plus 2 mM CaCl₂ (pH 7.4). Following perfusion, the cochleae were immersed in the above fixative for 2 h at room temperature. After the fixation, the organ of Corti was exposed by removing the bone from the apical coil, and the cochleae, then incubated in solutions of saturated aqueous thiocarbohydrazide (20 min) alternating with 1% osmium tetroxide in cacodylate buffer (2 h) twice (the OTOTO technique).⁸¹ (link) The cochleae were then dehydrated through an ethanol series and critical point dried using CO₂ as the transitional fluid (Leica EM CPD300) and mounted on specimen stubs using conductive silver paint (Agar Scientific, Stansted, UK). The apical coil of the organ of Corti was examined at 10 kV using a Tescan Vega3 LMU scanning electron microscope.

Jeng J.Y., Carlton A.J., Goodyear R.J., Chinowsky C., Ceriani F., Johnson S.L., Sung T.C., Dayn Y., Richardson G.P., Bowl M.R., Brown S.D., Manor U, & Marcotti W. (2022). AAV-mediated rescue of Eps8 expression in vivo restores hair-cell function in a mouse model of recessive deafness. Molecular Therapy. Methods & Clinical Development, 26, 355-370.

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