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Be0098

Manufactured by BioXCell
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The BE0098 is a high-performance centrifuge designed for a variety of laboratory applications. It features a brushless motor and a versatile rotor system that can accommodate multiple sample sizes and tube types. The centrifuge is capable of reaching speeds up to 15,000 rpm and can generate a maximum relative centrifugal force (RCF) of 21,380 x g. The temperature control system allows for precise temperature regulation during the centrifugation process.

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Lab products found in correlation

Isotype, by BioXCell (2 mentions) Rmp1 14 or, by BioXCell (2 mentions) Igg2a isotype ctrl, by BioXCell (2 mentions) Balb c mice, by Charles River Laboratories (2 mentions) Cutana chic cut run kit, by EpiCypher (1 mentions)

4 protocols using be0098

1

BATF3 Profiling in Pre-cDC Progenitors

Cited 2 times
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BATF3 CUT&RUN was performed with a CUTANA ChIC/CUT&RUN kit (EpiCypher) per the manufacturer's protocol, with modifications (Liu et al. 2022 (link)). To expand the pre-cDC1 and pre-cDC2 progenitors, Δ32/+ and Δ32/Δ41 mice were injected once daily, intraperitoneally, with 15 µg of recombinant Flt-3L-Ig (Bio X Cell BE0098) for eight consecutive days. Pre-cDC1 and pre-cDC2 progenitors were sort purified 24 h after the eighth dose of Flt-3L-Ig treatment. BATF3 CUT&RUN was performed with 1.0 × 106 cells using rabbit anti-BATF3 antibody (Grajales-Reyes et al. 2015 (link)). CUT&RUN libraries were prepared and data were analyzed as described (Liu et al. 2022 (link)).
Liu T.T., Ou F., Belk J.A., Bagadia P., Anderson DA I.I.I., Durai V., Yao W., Satpathy A.T., Murphy T.L, & Murphy K.M. (2023). Cis interactions in the Irf8 locus regulate stage-dependent enhancer activation. Genes & Development, 37(7-8), 291-302.
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2

Combination Therapy for Tumor Regression

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3x105 CT26 RTT cells were injected subcutaneously into the right flank of female 6-8-week-old Balb/c mice (Charles River). Animals were irradiated under anaesthesia with Hypnorm/Hypnovel (Fentanyl-Fluanisone/Midazolam), administered i.p. Irradiation was performed on day 14 using an AGO 250kV X-ray machine (AGO, Reading, UK). Radiation dose (9Gy) was measured using a Farmer Chamber and Unidos-E dosimeter. FLT3L administration was initiated on day 7 and administered in 10 consecutive doses of 30 μg i.p. (BE0098 or Isotype, BioXCell in PBS). 100 μg anti-PD-1 (RMP1-14 or IgG2a Isotype CTRL, BioXCell) were administered on day 15, 18, 21 and 24. Body weight and tumour growth were measured twice weekly and tumour volumes calculated as: length x width x height (mm) x 0.5236.
Haas L., Elewaut A., Gerard C.L., Umkehrer C., Leiendecker L., Pedersen M., Krecioch I., Hoffmann D., Novatchkova M., Kuttke M., Neumann T., de Silva I.P., Witthock H., Cuendet M.A., Carotta S., Harrington K.J., Zuber J., Scolyer R.A., Long G.V., Wilmott J.S., Michielin O., Vanharanta S., Wiesner T, & Obenauf A.C. (2021). Acquired resistance to anti-MAPK targeted therapy confers an immune-evasive tumour microenvironment and cross-resistance to immunotherapy in melanoma. Nature cancer, 2(7), 693-708.
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3

Expanding Dendritic Cells and Depleting NK Cells

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C57BL/6 and IL-10−/− female mice with vaginal plugs were treated i.p. with human recombinant FLT3 (BE0098, BioX cell, 10 μg/mouse/day) for 7 consecutive days to expand DC. Combined DC expansion and NK cell depletion was achieved in FLT3 treated female mice by injecting anti-asialo GM1 Ab (986–10001, WAKO, 2 μg/g BW) on ED4.5 at the same concentration as described above.
Blois S.M., Freitag N., Tirado-González I., Cheng S.B., Heimesaat M.M., Bereswill S., Rose M., Conrad M.L., Barrientos G, & Sharma S. (2017). NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface. Scientific Reports, 7, 2189.
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4

Combination Therapy for Tumor Regression

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3x105 CT26 RTT cells were injected subcutaneously into the right flank of female 6-8-week-old Balb/c mice (Charles River). Animals were irradiated under anaesthesia with Hypnorm/Hypnovel (Fentanyl-Fluanisone/Midazolam), administered i.p. Irradiation was performed on day 14 using an AGO 250kV X-ray machine (AGO, Reading, UK). Radiation dose (9Gy) was measured using a Farmer Chamber and Unidos-E dosimeter. FLT3L administration was initiated on day 7 and administered in 10 consecutive doses of 30 μg i.p. (BE0098 or Isotype, BioXCell in PBS). 100 μg anti-PD-1 (RMP1-14 or IgG2a Isotype CTRL, BioXCell) were administered on day 15, 18, 21 and 24. Body weight and tumour growth were measured twice weekly and tumour volumes calculated as: length x width x height (mm) x 0.5236.
Haas L., Elewaut A., Gerard C.L., Umkehrer C., Leiendecker L., Pedersen M., Krecioch I., Hoffmann D., Novatchkova M., Kuttke M., Neumann T., de Silva I.P., Witthock H., Cuendet M.A., Carotta S., Harrington K.J., Zuber J., Scolyer R.A., Long G.V., Wilmott J.S., Michielin O., Vanharanta S., Wiesner T, & Obenauf A.C. (2021). Acquired resistance to anti-MAPK targeted therapy confers an immune-evasive tumour microenvironment and cross-resistance to immunotherapy in melanoma. Nature cancer, 2(7), 693-708.
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