Western blots were performed as previously described (17 (link), 18 (link), 58 (link)) using NuPage 4 to 12% bis-tris gels and the following antibodies (at the manufacturer’s recommended concentrations): anti-TRPM7 (mouse, clone S74-25, Thermo Fisher Scientific MA5-27620), anti-Cdc42 (rabbit, clone 11A11, Cell Signaling Technology 2466), and anti-IQGAP1 (mouse, clone 9, Santa Cruz Biotechnology, sc-376021) with glyceraldehyde-3-phosphate dehydrogenase (rabbit, clone 14C10, Cell Signaling Technology 2118) or β-actin (mouse, clone C4, BD Biosciences 612656) as a loading control. Secondary antibodies (used at 1:1000 dilution): anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)–linked antibody (Cell Signaling Technologies) and anti-rabbit IgG HRP-linked antibody (Cell Signaling Technologies).
Anti mouse immunoglobulin g igg horseradish peroxidase hrp linked antibody
Manufactured by Cell Signaling Technology
Anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)–linked antibody is a secondary antibody used for detection in immunoassays. It binds to the primary mouse antibody and is conjugated with the enzyme horseradish peroxidase, allowing for colorimetric or chemiluminescent detection.
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Lab products found in correlation
2 protocols using anti mouse immunoglobulin g igg horseradish peroxidase hrp linked antibody
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of NKCC1 and NHE1
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Western blots were performed using NuPage 4% to 12% bistris gels and the following antibodies: anti NKCC1 (raised in rabbit; clone D13A9; Cell Signaling Technology 8351), and anti NHE1 (raised in mouse; clone 54; Santa Cruz Biotechnology; sc‐136239) with glyceraldehyde‐3‐phosphate dehydrogenase (rabbit, clone 14C10, Cell Signaling Technology 2118) as a loading control. Secondary antibodies used were anti‐mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)–linked antibody and anti‐rabbit IgG HRP‐linked antibody (both from Cell Signaling Technologies).
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