Prism 7b

For pairwise comparisons of cell migration speeds (Figures 3, 4, and 6c) and directionality ratios (Figure S1f, Supporting Information) of a control group with different perturbation conditions, we calculate p‐values using two‐sample t‐test as implemented by MATLAB's ttest2 function. Computation of the other statistical data was performed with KaleidaGraph 4.5.4 (Synergy Software) and Prism 7b (GraphPad Software, Inc). The numeric p‐values are denoted on the corresponding plots. If the p‐value is below 0.0001, i.e., cutoff lower limit for Kaleidagraph and Prism 7b, a ‘p < 0.0001′ is denoted. Sample size n (i.e., number of analyzed cells) for each measured parameter is denoted on the plots. Each measured parameter is a result of three replicates (independent experiments), unless specified otherwise. Box and whisker plots reflect the first quartile, median, third quartile, and 95% percent confidence interval. Data shown as violin diagrams are distributions. Mean and median values are outlined separately.

Multiple groups were compared by ANOVA, followed by the Tukey post hoc analysis. Pairwise comparisons were analyzed using a t test. Figure legends indicate which statistical test was performed for the data. Statistical analysis was performed using either KaleidaGraph 4.5.3 (Synergy Software) or Prism 7b (GraphPad Software, Inc). Sample size N for each comparison is reported in the corresponding plots (i.e., for FA size measurements ‘‘N’’ reflects the number of measured individual FAs across 5–10 randomly chosen cells). For overall cell morphology measurements, e.g., cell lengths, widths or averaging of the cell shapes, N represents the number of measured cells. Data are shown as mean ± s.d.

Differences in means between groups were evaluated by two-tailed Student’s t-tests performed in Prism 7b (Graphpad Software, Inc.). For multiple comparisons the differences were determined by using an analysis of variance (ANOVA) followed by Tukey post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and n.s. not significant. Unless otherwise stated, all data are presented as mean ± standard deviation (S.D.).

Pairwise comparisons were analyzed using a one-sided t-test. Multiple groups were compared by one-way analysis of variance, followed by the Tukey’s post hoc analysis. Figure legends indicate which statistical test was performed for the data. Statistical analysis was performed using either KaleidaGraph 4.5.4 (Synergy Software) or Prism 7b (GraphPad Software, Inc.). The exact p-values are indicated on the plots, unless the p < 0.0001, where 0.0001 is a cut-off lower limit for Kaleidagraph and Prism softwares. Sample size n for each comparison is reported in the corresponding plots (i.e., n reflects the number of measured individual cells or the cells’ movement steps per given time interval). Number of replicates is three or more, unless specified otherwise. Data are shown as box and whiskers diagrams: 95% confidence interval, first quartile, median, third quartile, and 95% confidence interval.