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Foxp3egfp cre ert2 mice

Manufactured by Jackson ImmunoResearch

Foxp3eGFP-Cre-ERT2 mice are a transgenic mouse line that expresses an enhanced green fluorescent protein (eGFP) and a tamoxifen-inducible Cre recombinase (Cre-ERT2) under the control of the Foxp3 promoter. This allows for the specific labeling and manipulation of Foxp3-expressing regulatory T cells (Tregs) in vivo.

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3 protocols using foxp3egfp cre ert2 mice

1

Genetic Manipulation of Murine Models

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All mice used in this study were maintained in SPF or Germ-free facilities at the University of Florida. The mice were littermate controlled and were 6-8 weeks old for experiments unless otherwise noted. Tfamfl/fl mice were kindly provided by Navdeep Chandel (Northwestern University). Foxp3Yfp-Cre, Foxp3eGFP-Cre-ERT2 mice, CD45.1homo, Rag1−/− and ROSA26Stop-YFP mice were purchased from Jackson Laboratory. Cd4-Cre mice were purchased from Taconic Farms and breed in University of Florida. Experiments were performed with both male and female littermate animals of each genotype unless otherwise indicated. All studies with mice were approved by the Animal Care and Use Committees of the University of Florida.
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2

Transgenic Mouse Models for Immunology Research

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Foxp3YFP-cre mice (stock number 016959) and Foxp3eGFP-Cre-ERT2 mice (stock number 016961) were purchased from the Jackson Laboratory. Rosa26Stat5b-CA mice were provided by Dr. Alexander Rudensky at Memorial Sloan Kettering Cancer Center (10 (link)). Ogtfl/fl Mice (Jackson Laboratory stock number 004860) were kindly provided by Dr. Xiaoyong Yang at Yale University. All mice were on C57BL/6 background. Mice were free to access water and fed on a regular chow, tamoxifen food (Teklad, TD.130860), or 60% high fat diet (ResearchDiets, D12492) as indicated. All procedures were approved by the Institutional Animal Care and Use Committee at the University of Minnesota. All relevant ethical regulations for animal testing and research were complied with.
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3

Genetic Manipulation of Murine Models

Check if the same lab product or an alternative is used in the 5 most similar protocols
All mice used in this study were maintained in SPF or Germ-free facilities at the University of Florida. The mice were littermate controlled and were 6-8 weeks old for experiments unless otherwise noted. Tfamfl/fl mice were kindly provided by Navdeep Chandel (Northwestern University). Foxp3Yfp-Cre, Foxp3eGFP-Cre-ERT2 mice, CD45.1homo, Rag1−/− and ROSA26Stop-YFP mice were purchased from Jackson Laboratory. Cd4-Cre mice were purchased from Taconic Farms and breed in University of Florida. Experiments were performed with both male and female littermate animals of each genotype unless otherwise indicated. All studies with mice were approved by the Animal Care and Use Committees of the University of Florida.
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