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T3 or t7 mmessage mmachine kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The T3 or T7 mMESSAGE mMACHINE Kit is a laboratory tool used for the in vitro transcription of capped and polyadenylated messenger RNA (mRNA) from DNA templates. The kit contains the necessary components, including the appropriate RNA polymerase, to generate high-quality mRNA for various applications, such as gene expression studies, mRNA therapeutics, and more.

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T7 or T3 mMESSAGE mMACHINE kits T3 mMESSAGE mMACHINE T7 mMessage mMachine MMessage mMachine kit T3 mMESSAGE Kit T7 mMachine kit MMessage T7 kit MMessage mMachine T7 kits

2 protocols using t3 or t7 mmessage mmachine kit

1

RNAi Screening and RNA Injection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RNAi screen was performed using the Ahringer-MRC feeding RNA interference library (Source BioScience, Nottingham, UK) and feeding protocol (Kamath et al., 2003 (link)). Other RNAi experiments were done using injection of double-stranded RNA synthesized from PCR-amplified genomic fragments using a T3 or T7 mMESSAGE mMACHINE Kit (Ambion).
Vuong-Brender T.T., Suman S.K, & Labouesse M. (2017). The apical ECM preserves embryonic integrity and distributes mechanical stress during morphogenesis. Development (Cambridge, England), 144(23), 4336-4349.
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2

RNAi Experiments Using Injection

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RNAi experiments were done using injection of double-stranded RNA synthesized from PCR-amplified genomic fragments using a T3 or T7 mMESSAGE mMACHINE Kit (Ambion, Austin, TX, USA). The embryos were analyzed from 24 hr to 48 hr post-injection.
Vuong-Brender T.T., Ben Amar M., Pontabry J, & Labouesse M. (2017). The interplay of stiffness and force anisotropies drives embryo elongation. eLife, 6, e23866.
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