Hosepic

Ovarian cancer cell line SKOV3 (cat no. ATCC^® HTB-77™) was obtained from American Type Culture Collection. Non-tumorous human ovarian surface epithelial cells (HOSEpiC; cat no. BNCC340096) were purchased from the BeNa Culture Collection (Suzhou Bei Na Chuanglian Biotechnology Co., Ltd.). SKOV3 and HOSEpiC cells were cultured in complete Dulbecco's Modified Eagle Medium (DMEM)/nutrient mixture F12 (Gibco, Thermo Fisher Scientific, Inc.) including 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin solution at 37°C in a 5% CO₂ incubator.

The ovarian cancer cell lines SK-OV-3 and HO8910 (iCell Bioscience Inc,Shanghai, China) and normal human ovarian surface epithelial cell line HOSEPIC (Typical Culture Preservation Commission CellBank, Chinese Academy of Sciences, Shanghai, China) were used in this study. The SK-OV-3, HOSEPIC and HO8910 cells were cultured in McCoy’s 5A medium (Catalog #16600-082, Gibco, Carlsbad, CA, USA), DMEM F12 medium (Catalog #10–092-CVR, Corning, Inc., Corning, NY, USA), and RPMI 1640 medium (Catalog #10–040-CV, Corning, Inc.,), respectively, supplemented with 10% FBS and 1% P/S (Sangon Biotech, Shanghai, China). The cells were seeded into 6-well plates at a density of 3×10⁵ cells per well and grown for 24 h in complete medium until the fusion rate reached 80–90%. Then, the cells were rinsed with fresh medium and transfected with the tRF03357 mimics, mimics NC, tRF03357 inhibitor,or inhibitor NC using Lipofectamine^TM2000 (Invitrogen, CA, USA) according to the manufacturer’s protocol. The cells were cultured for 24 h and then used for the follow-up experiments.

Human ovarian cancer cell lines, SKOV3 and ES-2 were obtained from the American Type Culture Collection, and human ovarian surface epithelial cells (HOSEpiC) were purchased from the BeNa Cell Culture Collection (cat. no. BNCC340096). SKOV3 and ES-2 cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37˚C with 5% CO₂. HOSEpiC cells were cultured in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS.
SKOV3 cells were transfected with inhibitor control, miR-361-5p inhibitor, TRAF3-short-hairpin (sh)RNA, control-shRNA, miR-361-5p inhibitor + control-shRNA or miR-361-5p inhibitor + TRAF3-shRNA for 48 h using Lipofectamine 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. A period of 48 h after cell transfection, transfection efficiency was detected using reverse transcription-quantitative (RT-q)PCR.

The cisplatin-sensitive human ovarian cancer cell lines OV2008 and its cisplatin-resistant clone C13K were supplied by Dr. Wen-cheng Ding (Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology). The human epithelial ovarian cancer cell lines SKOV3, A2780, and normal human ovarian surface epithelial cell line human ovarian surface epithelial cells (HOSEpiC) were purchased from the Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences (Shanghai, China). OV2008, C13K, and HOSEpiC cells were maintained in complete RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS). In contrast, the SKOV3 and A2780 cell lines were cultured in Dulbecco's modified eagle medium (DMEM, Thermo Fisher Scientific) medium with 10% FBS serum. All cells were cultured at 37°C in 5% CO₂.

The HOC cell lines COC1, A2780 and SKOV3 and the normal human ovarian epithelial cell line HOSEpiC were purchased from National Collection of Authenticated Cell Cultures of The Chinese Academy of Sciences and incubated in cell culture dishes at a density of 1×10⁵ cells/cm². The cells were cultured in RPMI-1640 medium (COC1 and SKOV3), DMEM (A2780) or minimum essential medium (MEM; HOSEpiC) (all purchased from Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Hyclone; Cytiva) at 37°C with 5% CO₂ for 48 h. When confluence reached 80–90%, cells were detached with 0.025% trypsin (Gibco; Thermo Fisher Scientific, Inc.) and passaged.