Round coverslips

All the oligonucleotides in this work were obtained from Huzhou Hippo Biotechnology Co., Ltd. (Huzhou, China), and the oligonucleotides used in this work are listed in Supplementary Table S1. T4 DNA ligase and Phi29 DNA polymerase were purchased from New England BioLabs (Beverly, MA, United States). Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum were purchased from Gibco. Cell culture dishes/plates, round coverslips, and centrifuge tubes were obtained from NEST Biotechnology Co. Ltd. (Wuxi, China). Hoechst 33,342 was purchased from Abbkine Scientific (Wuhan, China). Calcein-AM staining kit was purchased from Solarbio kit (Beijing, China). Annexin V-fluorescein-5-isothiocyanate (AV-FITC)/PI double staining kit was purchased from Elabscience Biotechnology Co., Ltd.

The adult retinal pigment epithelial cell line-19 (ARPE-19) and the human invasive UVM cell line (C918) were purchased from Procell Life Science&Technology Co., Ltd (Wuhan, Hubei, China). ARPE-19 cell was cultured in Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F12 Ham’s Liquid media (DMEM/F-12; Cytiva/Global Life Sciences Solutions, Marlborough, MA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), along with 100 U/mL penicillin and streptomycin (Gibco, Carlsbad, CA, USA). C918 cell was cultured in Roswell Park Memorial Institute 1640 liquid media (RPMI1640; Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), along with 100 U/mL penicillin and streptomycin (Gibco, Carlsbad, CA, USA). Cells were maintained in an incubator (Thermo Fisher Scientific, Waltham, MA) at 37°C, with 95% humidity, and 5% CO2. Cell culture plates, round coverslips, and centrifuge tubes were obtained from (NEST Biotechnology; Wuxi, China). C918 cells were transfected with the generated small interfering RNAs (RiboBio; Guangzhou, China) targeting gene S100A13 and its control siRNAs, according to the manufacturer’s procedure. The siRNA sequences for the gene S100A13 were ACTCGGAGCTCAAGTTCAA.