LPS (E. Coli 0111:B4), dopamine hydrochloride, fenoldopam, and pergolide were purchased from Sigma-Aldrich® (Saint Louis, MO) and dissolved in sterile, pyrogen-free PBS (Gibco®, Life Technologies, Grand Island, NY). The glucose measuring strips were purchased from PharmaTech Solutions, Inc. (Westlake village, CA). Butaclamol (15 mg/kg body weight i.p.) was given 90 min before and/or after exercise. Pentobarbital sodium was purchased from Diamondback (Scottsdale, AZ); ketamine from Henry Schein animal health (Dublin, OH); xylazine from Akorn animal health (Lake Forest, IL, USA) and enrofloxacine from Bayer Health care (Shawnee Mission, KS, USA).
Fenoldopam
Manufactured by Merck Group
Sourced in Macao, United States
Fenoldopam is a laboratory equipment product manufactured by Merck Group. It is a selective dopamine D1 receptor agonist, which is used in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Lps e coli 0111 b4, by Merck Group (4 mentions)
Pergolide, by Merck Group (3 mentions)
Dopamine hydrochloride, by Merck Group (3 mentions)
Ketamine, by Henry Schein (3 mentions)
Streptozotocin (stz), by Merck Group (3 mentions)
Xylazine, by Akorn (2 mentions)
Sch23390, by Merck Group (2 mentions)
Reserpine, by Merck Group (2 mentions)
Fusaric acid, by Merck Group (2 mentions)
Dopamine, by Merck Group (2 mentions)
Butaclamol, by Merck Group (2 mentions)
Capsaicin, by Merck Group (2 mentions)
Enrofloxacin, by Bayer (2 mentions)
Enrofloxacine, by Bayer (1 mentions)
Rosiglitazone maleate, by Merck Group (1 mentions)
Pd98059, by Merck Group (1 mentions)
Lps escherichia coli 0111 b4, by Merck Group (1 mentions)
Candesartan, by Merck Group (1 mentions)
Diphenyleneiodonium, by Merck Group (1 mentions)
Nadph, by ICN Biomedicals (1 mentions)
9 protocols using fenoldopam
1
Modulation of Glycemic Responses
2
Antibody Detection of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antibodies (GRK4 and D1R) were rabbit anti‐rat antibodies and were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX) and Millipore (Billerica, MA). The phosphoserine antibody was a rabbit anti‐rat antibody and was purchased from Cell Signaling Technology (Beverly, MA). Fenoldopam, a D1‐like receptor agonist, was purchased from Sigma (St. Louis, MO). All other chemicals for the various buffers were of the highest purity available and purchased from Sigma or Gibco (Grand Island, NY).
3
Investigating Dopaminergic Receptor Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fenoldopam, rosiglitazone maleate, STZ, mitogen-activated protein kinase (MAPK) inhibitor PD98059, Ach, SNP, PHE, and D1 receptor antagonist SCH23390 were obtained from Sigma-Aldrich (St. Louis, MO). Rabbit anti-rat D1 receptor polyclonal antibodies and rat insulin 96 well plate assay kit were obtained from Millipore Corporation (St Charles, MO); and anti-phosphoserine antibodies were obtained from Abcam Ltd (New Territories, Hong Kong, China).
4
In Vivo and In Vitro Endotoxemia Models
5
Differentiation and Pharmacological Stimulation of LLCPK Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LLCPK cells plated on sterile glass-bottom plates were cultured to a confluent monolayer in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum at 37°C in 5% CO2. Serum was then withdrawn from cells to allow differentiation. A faster differentiation could be achieved by cell-cell contact inhibiting cell growth and/or withdrawing serum from the cell culture media. Because only fully-differentiated cells have well-developed cilia, cell differentiation can be validated by immunostaining of the sensory proteins in the cilia or responsiveness of the cells to fluid-shear stress. We have previously discussed cell differentiation in details (Nauli et al., 2013 (link)). Once differentiated, dopamine or fenoldopam (SigmaAldrich, Inc.) were gently added to culture plates to achieve 1 nM to 10 μM final concentrations for 16 h in the absence of serum. For control experiments, vehicle alone was added to cells in the same manner and volume as those contained pharmacological agents.
6
Fenoldopam Treatment in Sepsis Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LPS (Escherichia coli 0111:B4), streptozotocin, glucose, dopamine hydrochloride, and fenoldopam were purchased from Sigma-Aldrich® (Saint Louis, MO, USA). The glucose measuring strips were purchased from PharmaTech Solutions, Inc. (Westlake Village, CA, USA). Pentobarbital sodium was purchased from Diamondback (Scottsdale, AZ, USA); ketamine from Henry Schein animal health (Dublin, OH, USA); xylazine from Akorn animal health (Lake Forest, IL, USA), and enrofloxacin from Bayer Healthcare (Shawnee Mission, KS, USA). streptozotocin was injected (STZ; i.p., 50 mg/kg) at 10 and 5 days before the experiment as previously reported (35 (link), 36 (link)). Treatment with fenoldopam (Fen; 10 mg/kg/dose; i.p.) was administered at 6 and 1 h before LPS or CLP in most experiments. Treatment with fenoldopam was started 15 h after CLP and given every 12 h for 3 days in the survival experiments.
7
In Vivo and In Vitro Endotoxemia Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LPS (E. coli 0111:B4), capsaicin, reserpine, fusaric acid, dopamine, fenoldopam, pergolide and butaclamol were all purchased from Sigma-Aldrich® (Saint Louis, MO) and dissolved in sterile, pyrogen-free PBS (Gibco®, Life Technologies, Grand Island, NY). capsaicin (5 mg kg−1 i.m.; dissolved in PBS with 10% ethyl alcohol) was administered at 72 h and 48 h before the LPS challenge. reserpine (5 mg kg−1; i.p.; dissolved in 25% DMSO-PBS) was injected at 24 h before the lethal endotoxemia. fusaric acid (40 mg kg−1 per dose; i.p.) was administered at 8 h and 4 h before the LPS challenge. fenoldopam (10 mg kg−1 per dose; i.p.) was injected at 30 min and 5 min before LPS administration. pergolide (10 mg kg−1 per dose; i.p.; dissolved in 25% DMSO-PBS) was given at 12 h and 5 min before the LPS challenge. butaclamol (12 mg kg−1, i.p) was given 60 min before the endotoxemic challenge. In vitro; dopamine, fenoldopam, pergolide and butaclamol were used at 5 min before the LPS challenge at the concentration indicated in the figures.
8
LPS-Induced Inflammation: Dopamine and Fenoldopam
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LPS (E. Coli 0111: B4), dopamine hydrochloride, fenoldopam, streptozotocin, and glucose were purchased from Sigma-Aldrich® (Saint Louis, MO). The glucose measuring strips were purchased from PharmaTech Solutions, Inc (Westlake Village, CA). Pentobarbital sodium was purchased from Diamondback (Scottsdale, AZ); ketamine from Henry Schein animal health (Dublin, OH); xylazine from Akron animal health (Lake Forest, IL, USA) and enrofloxacin from Bayer Healthcare (Shawnee Mission, KS, USA). Dopamine (DA; 10 mg/kg/dose; i.p.) or fenoldopam (Fen; 10 mg/kg/dose; i.p.) were administered at 6 and 1 h before LPS.
9
Cytochrome C Oxidative Stress Assay
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!