HPLC analysis was performed using a C18 Onyx Monolithic column 100 mm × 4.6 mm (Phenomenex, Torrance, CA, USA) coupled in sequence to a C18 Chromolith Performance RP-18e column 100 mm × 4.6 mm (Merck, Darmstadt, Germany) in a chromatographic system consisting of a System Gold Programmable Solvent Module 126 Pump (Beckman, San Ramon, CA, USA) equipped with an HT 800 L autosampler (HTA, Brescia, Italy) fitted with a 20 µl loop. The FLD detection was obtained by means of a 821 FP fluorescence detector (Jasco, Tokyo, Japan) set at the excitation wavelength of 340 nm and emission wavelength of 460 nm. The system was computer-controlled by a Beckman Coulter 32 Karat Software (Beckman, San Ramon, CA, USA). OTA separation was archived using isocratic elution and at room temperature, under conditions similar to those adopted by Altafini et al. [21 (link)]. The mobile phase consisted of 70% of a mixture of water, isopropyl alcohol-acetonitrile and acetic acid 1% (79:7:7:7 v/v) and 30% of acetonitrile. The flow rate was set at 1 mL/min, while the injection volume was 20 µL.
System gold programmable solvent module 126 pump
Manufactured by Beckman Coulter
Sourced in United States
The System Gold Programmable Solvent Module 126 Pump is a high-performance liquid chromatography (HPLC) pump. It is designed to deliver precise and reliable solvent flow for HPLC applications. The pump features programmable solvent delivery, enabling users to create complex solvent gradient profiles. It is a core component of HPLC systems, responsible for the accurate and reproducible delivery of mobile phase solvents.
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Lab products found in correlation
4 protocols using system gold programmable solvent module 126 pump
1
HPLC Analysis of Ochratoxin A
2
Aflatoxin M1 Analysis by HPLC-FLD
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AFM1 analysis was performed by HPLC-FLD on a chromatographic apparatus consisting of a System Gold Programmable Solvent Module 126 Pump (Beckman, San Ramon, CA, USA) equipped with an HT 800 L autosampler (HTA, Brescia, Italy) fitted with a 100 µL loop. Chromatographic separation was achieved in isocratic elution mode and at room temperature using an analytical column Zorbax Eclipse Plus C18 150 × 4.6 mm 5 µm (Agilent, Santa Clara, CA, USA). The mobile phase consisted of 61% of deionized water, and 39% of acetonitrile. The flow rate was set at 0.7 mL/min, and the injection volume was 100 µL. The FLD detection was obtained by means of a 821 FP fluorescence detector (Jasco, Tokyo, Japan) set at the excitation wavelength of 360 nm and emission wavelength of 440 nm. The system was computer-controlled by a Beckman Coulter 32 Karat Software.
3
HPLC Quantification of Analytes
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For OTA analysis and quantification, a HPLC system consisting of a System Gold Programmable Solvent Module 126 pump (Beckman Coulter, Brea, CA, USA) equipped with an HT 800 L autosampler (HTA, Brescia, Italy) fitted with a 20 l loop and an 821 FP fluorescence detector (Jasco, Tokyo, Japan) was used. Fluorescence excitation and emission wavelengths were 340 and 460 nm respectively. The system was computer-controlled by a Beckman Coulter 32 Karat Software. The analytical conditions were those developed by Armorini et al. (2016) , and optimized for use in this study.
Chromatographic separation was achieved in gradient elution mode using two monolithic columns coupled in sequence: a C18 Onyx Monolithic 100 mm x 4.6 mm (Phenomenex, Torrance, CA, USA) and a C18 Chromolith Performance RP-18e 100 mm x 4.6 mm (Merck, Darmstad, Germany). The mobile phase consisted of eluent A containing water/acetonitrile/isopropyl alcohol/acetic acid 1% (79:7:7:7, v/v) and eluent B consisting of acetonitrile. The gradient program started with 58% A and 42% B, reaching 49% B in 3.5 min with linear increase. The flow rate was 1.1 ml/min, while the sample injection volume was 20 µl.
Chromatographic separation was achieved in gradient elution mode using two monolithic columns coupled in sequence: a C18 Onyx Monolithic 100 mm x 4.6 mm (Phenomenex, Torrance, CA, USA) and a C18 Chromolith Performance RP-18e 100 mm x 4.6 mm (Merck, Darmstad, Germany). The mobile phase consisted of eluent A containing water/acetonitrile/isopropyl alcohol/acetic acid 1% (79:7:7:7, v/v) and eluent B consisting of acetonitrile. The gradient program started with 58% A and 42% B, reaching 49% B in 3.5 min with linear increase. The flow rate was 1.1 ml/min, while the sample injection volume was 20 µl.
4
HPLC Analysis of Fluorescent Compounds
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The HPLC system consisted of a System Gold Programmable Solvent Module 126 pump (Beckman Coulter, Brea, CA, USA) equipped with an HT 800 L autosampler (HTA, Brescia, Italy) fitted with a 20 μl loop and a 821 FP fluorescence detector (Jasco, Tokyo, Japan); fluorescence excitation and emission wavelengths were 340 and 460 nm respectively. The system was computer-controlled by a Beckman Coulter 32 Karat Software. The HPLC column was a C18 Onyx Monolithic column 100 mm x 4.6 mm (Phenomenex, Torrance, CA, USA) coupled in sequence to a C18 Chromolith Performance RP-18e column 100 mm x 4.6 mm (Merck, Darmstad, Germany). Chromatographic separation was achieved in gradient conditions and at room temperature. The mobile phase consisted of water-acetonitrile-isopropyl alcohol-acetic acid 1% mixtures in various ratios. Mobile phase A: water/acetonitrile/isopropyl alcohol/acetic acid 1% (79:7:7:7 v/v) and mobile phase B: acetonitrile. Gradient: from 80% A and 20% B to 58% A and 42% B in 11 min with linear increase. Flow rate: 1 ml/min. The injection volume was 20 µl.
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